LOCUS Exported 14643 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION A non-leaky heat-inducible gene expression system for Drosophila. (GenBank: MF058598.1). ACCESSION . VERSION . KEYWORDS PRExpress SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 14643) AUTHORS Akmammedov A, Geigges M, Paro R TITLE Single vector non-leaky gene expression system for Drosophila melanogaster. JOURNAL Sci Rep. 2017 Jul 31;7(1):6899. doi: 10.1038/s41598-017-07282-w. PUBMED 28761084 REFERENCE 2 (bases 1 to 14643) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..14643 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 122..3169 /codon_start=1 /gene="lacZ" /product="beta-galactosidase" /label=lacZ /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD SWSPSVSAELQLSAGRYHYQLVWCQK" protein_bind 3619..4004 /label=gypsy insulator /bound_moiety="Su(Hw), CP190, and Mod(mdg4)67.2" /note="chromatin insulator from Drosophila" CDS complement(join(4812..5426,5497..5628,5832..6147,6209..6863, 6938..7211,7613..7684)) /codon_start=1 /gene="white" /product="Drosophila white gene eye color pigment" /label=mini-white /note="This is a modified version of the white gene lacking part of the first intron." /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE" primer_bind 8136..8154 /label=pCasper-F /note="Drosophila mini-white gene, reverse primer" primer_bind complement(8187..8206) /label=T7 /note="T7 promoter, forward primer" promoter complement(8188..8206) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(8213..8230) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(8213..8229) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin 8371..8826 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(8458..8477) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 8668..8689 /label=F1ori-F /note="F1 origin, forward primer" promoter 8852..8956 /gene="bla" /label=AmpR promoter CDS 8957..9817 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(9175..9194) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 9988..10576 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 10477..10496 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 10730..10747 /label=L4440 /note="L4440 vector, forward primer" protein_bind 10864..10885 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 10900..10930 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10938..10954 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 10943..10965 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 10962..10978 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 10962..10978 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" promoter 10999..11017 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" protein_bind 11236..11305 /label=attB /bound_moiety="phage phi-C31 integrase" /note="attB site for the phi-C31 integrase (Groth et al., 2000)" protein_bind 11345..11730 /label=gypsy insulator /bound_moiety="Su(Hw), CP190, and Mod(mdg4)67.2" /note="chromatin insulator from Drosophila" promoter 14191..14643 /label=hsp70 promoter /note="Drosophila melanogaster hsp70Bb promoter" primer_bind 14590..14611 /label=pCasper-hs /note="Drosophila Hsp70 promoter, forward primer" ORIGIN 1 aatagggaat tgggaattcg ccaccatgtc gtttactttg accaacaaga acgtgatttt 61 cgttgccggt ctgggaggca ttggtctgga caccagcaag gagctgctca agcgcgatcc 121 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc 181 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc 241 ccaacagttg cgcagcctga atggcgaatg gcgctttgcc tggtttccgg caccagaagc 301 ggtgccggaa agctggctgg agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc 361 aaactggcag atgcacggtt acgatgcgcc catctacacc aacgtgacct atcccattac 421 ggtcaatccg ccgtttgttc ccacggagaa tccgacgggt tgttactcgc tcacatttaa 481 tgttgatgaa agctggctac aggaaggcca gacgcgaatt atttttgatg gcgttaactc 541 ggcgtttcat ctgtggtgca acgggcgctg ggtcggttac ggccaggaca gtcgtttgcc 601 gtctgaattt gacctgagcg catttttacg cgccggagaa aaccgcctcg cggtgatggt 661 gctgcgctgg agtgacggca gttatctgga agatcaggat atgtggcgga tgagcggcat 721 tttccgtgac gtctcgttgc tgcataaacc gactacacaa atcagcgatt tccatgttgc 781 cactcgcttt aatgatgatt tcagccgcgc tgtactggag gctgaagttc agatgtgcgg 841 cgagttgcgt gactacctac gggtaacagt ttctttatgg cagggtgaaa cgcaggtcgc 901 cagcggcacc gcgcctttcg gcggtgaaat tatcgatgag cgtggtggtt atgccgatcg 961 cgtcacacta cgtctgaacg tcgaaaaccc gaaactgtgg agcgccgaaa tcccgaatct 1021 ctatcgtgcg gtggttgaac tgcacaccgc cgacggcacg ctgattgaag cagaagcctg 1081 cgatgtcggt ttccgcgagg tgcggattga aaatggtctg ctgctgctga acggcaagcc 1141 gttgctgatt cgaggcgtta accgtcacga gcatcatcct ctgcatggtc aggtcatgga 1201 tgagcagacg atggtgcagg atatcctgct gatgaagcag aacaacttta acgccgtgcg 1261 ctgttcgcat tatccgaacc atccgctgtg gtacacgctg tgcgaccgct acggcctgta 1321 tgtggtggat gaagccaata ttgaaaccca cggcatggtg ccaatgaatc gtctgaccga 1381 tgatccgcgc tggctaccgg cgatgagcga acgcgtaacg cgaatggtgc agcgcgatcg 1441 taatcacccg agtgtgatca tctggtcgct ggggaatgaa tcaggccacg gcgctaatca 1501 cgacgcgctg tatcgctgga tcaaatctgt cgatccttcc cgcccggtgc agtatgaagg 1561 cggcggagcc gacaccacgg ccaccgatat tatttgcccg atgtacgcgc gcgtggatga 1621 agaccagccc ttcccggctg tgccgaaatg gtccatcaaa aaatggcttt cgctacctgg 1681 agagacgcgc ccgctgatcc tttgcgaata cgcccacgcg atgggtaaca gtcttggcgg 1741 tttcgctaaa tactggcagg cgtttcgtca gtatccccgt ttacagggcg gcttcgtctg 1801 ggactgggtg gatcagtcgc tgattaaata tgatgaaaac ggcaacccgt ggtcggctta 1861 cggcggtgat tttggcgata cgccgaacga tcgccagttc tgtatgaacg gtctggtctt 1921 tgccgaccgc acgccgcatc cagcgctgac ggaagcaaaa caccagcagc agtttttcca 1981 gttccgttta tccgggcaaa ccatcgaagt gaccagcgaa tacctgttcc gtcatagcga 2041 taacgagctc ctgcactgga tggtggcgct ggatggtaag ccgctggcaa gcggtgaagt 2101 gcctctggat gtcgctccac aaggtaaaca gttgattgaa ctgcctgaac taccgcagcc 2161 ggagagcgcc gggcaactct ggctcacagt acgcgtagtg caaccgaacg cgaccgcatg 2221 gtcagaagcc gggcacatca gcgcctggca gcagtggcgt ctggcggaaa acctcagtgt 2281 gacgctcccc gccgcgtccc acgccatccc gcatctgacc accagcgaaa tggatttttg 2341 catcgagctg ggtaataagc gttggcaatt taaccgccag tcaggctttc tttcacagat 2401 gtggattggc gataaaaaac aactgctgac gccgctgcgc gatcagttca cccgtgcacc 2461 gctggataac gacattggcg taagtgaagc gacccgcatt gaccctaacg cctgggtcga 2521 acgctggaag gcggcgggcc attaccaggc cgaagcagcg ttgttgcagt gcacggcaga 2581 tacacttgct gatgcggtgc tgattacgac cgctcacgcg tggcagcatc aggggaaaac 2641 cttatttatc agccggaaaa cctaccggat tgatggtagt ggtcaaatgg cgattaccgt 2701 tgatgttgaa gtggcgagcg atacaccgca tccggcgcgg attggcctga actgccagct 2761 ggcgcaggta gcagagcggg taaactggct cggattaggg ccgcaagaaa actatcccga 2821 ccgccttact gccgcctgtt ttgaccgctg ggatctgcca ttgtcagaca tgtatacccc 2881 gtacgtcttc ccgagcgaaa acggtctgcg ctgcgggacg cgcgaattga attatggccc 2941 acaccagtgg cgcggcgact tccagttcaa catcagccgc tacagtcaac agcaactgat 3001 ggaaaccagc catcgccatc tgctgcacgc ggaagaaggc acatggctga atatcgacgg 3061 tttccatatg gggattggtg gcgacgactc ctggagcccg tcagtatcgg cggaattaca 3121 gctgagcgcc ggtcgctacc attaccagtt ggtctggtgt caaaaataag gatccgtcga 3181 ctaaggccaa agagtctaat ttttgttcat caatgggtta taacatatgg gttatattat 3241 aagtttgttt taagtttttg agactgataa gaatgtttcg atcgaatatt ccatagaaca 3301 acaatagtat tacctaatta ccaagtctta atttagcaaa aatgttattg cttatagaaa 3361 aaataaatta tttatttgaa atttaaagtc aacttgtcat ttaatgtctt gtagactttt 3421 gaaagtctta cgatacaatt agtatctaat atacatgggt tcattctaca ttctatatta 3481 gtgatgattt ctttagctag taatacattt taattatatt cggctttgat gattttctga 3541 ttttttccga acggattttc gtagaccctt tcgatctcat aatggctcat tttattgcga 3601 tggatcagga gcggccgcaa ttgatcggct aaatggtatg gcaagaaaag gtatgcaata 3661 taataatctt ttattgggta tgcaacgaaa atttgtttcg tcaacgtatg caatattttt 3721 tattaaaaga gggtatgcaa tgtattttat taaaaacggg tatgcaatat aataatcttt 3781 tattgggtat gcaacgaaaa tttgtttcgt caaagtatgc aatatttttt attaaaagag 3841 ggtatgcaat gtattttatt aaaaacgggt atgcaataaa aaattatttg gtttctctaa 3901 aaagtatgca gcacttattt tttgataagg tatgcaacaa aattttactt tgccgaaaat 3961 atgcaatgtt tttgcgaata aattcaacgc acacttatta cgtgacctgc aggcatgcaa 4021 gcatttaaat cctaagctct agcctagttc cagtgaaatc caagcatttt ctaaattaaa 4081 tgtattctta ttattatagt tgttattttt gatatatata aacaacacta ttatgcccac 4141 catttttttg agatgcatct acacaaggaa caaacactgg atgtcacttt cagttcaaat 4201 tgtaacgcta atcactccga acaggtcaca aaaaattacc ttaaaaagtc ataatattaa 4261 attagaataa atatagctgt gagggaaata tatacaaata tattggagca aataaattgt 4321 acatacaaat atttattact aatttctatt gagacgaaat gaaccactcg gaaccatttg 4381 agcgaaccga atcgcgcgga actaacgaca gtcgctccaa ggtcgtcgaa caaaaggtga 4441 atgtgttgcg gagagcgggt gggagacagc gaaagagcaa ctacgaaacg tggtgtggtg 4501 gaggtgaatt atgaagaggg cgcgcgattt gaaaagtatg tatataaaaa atatatcccg 4561 gtgttttatg tagcgataaa cgagtttttg atgtaaggta tgcaggtgtg taagtctttt 4621 ggttagaaga caaatccaaa gtctacttgt ggggatgttc gaaggggaaa tacttgtatt 4681 ctataggtca tatcttgttt ttattggcac aaatataatt acattagctt tttgaggggg 4741 caataaacag taaacacgat ggtaataatg gtaaaaaaaa aaaacaagca gttatttcgg 4801 atatatgtcg gctactcctt gcgtcgggcc cgaagtctta gagccagata tgcgagcacc 4861 cggaagctca cgatgagaat ggccagaccc acgtagtcca gcggcagatc ggcggcggag 4921 aagttaagcg tctccaggat gaccttgccc gaactggggc acgtggtgtt cgacgatgtg 4981 cagctaattt cgcccggctc cacgtccgcc cattggttaa tcagcagacc ctcgttggcg 5041 taacggaacc atgagaggta cgacaaccat ttgaggtata ctggcaccga gcccgagttc 5101 aagaagaagc cgccaaagag caggaatggt atgataaccg gcggacccac agacagcgcc 5161 atcgaggtcg aggagctggc gcaggatatt agatatccga aggacgttga cacattggcc 5221 accagagtga ccagcgccag gcagttgaag aagtgcagca ctccggcccg cagtccgatc 5281 atcggatagg caatcgccgt gaagaccagt ggcactgtga gaaaaagcgg caattcggca 5341 atcgttttgc ccagaaagta tgtgtcacag cgataaagtc gacttcgggc ctccctcata 5401 aaaactggca gctctgaggt gaacacctaa atcgaatcga ttcattagaa agttagtaaa 5461 ttattgaaat gcaaatgtat tctaaacatg acttacattt atcgtggcaa agacgttttg 5521 aaaggtcatg ttggtcagga agaggaagat ggctccgttg atattcatca cacccacttg 5581 cgtgagttgt tggcccaaaa agatgaggcc aatcaagatg gcaaccatct gcaaattaaa 5641 atgttactcg catctcatta atattcgcga gttaaatgaa atttatttat cttctgcaaa 5701 actataaact atacatctca ttgaaaaaaa ctaagaaggg tgtggaatca ggcaattcta 5761 tctaaaatct agcgaatttg tttccaagaa ttgtaagcgt tatatcattt gtttccactg 5821 gaaccactca ccgttgtctg aataagtcgc acttttacga ggagtggttc cttgagcacc 5881 gacagccagg atcgccacag gaccgcccgg aactgcatga accaggtggc cttgtaggtg 5941 tacccattct ccggctgctc cagtggcttc tccagatttt tggtggccaa caactgctcc 6001 atatcccggg ctactttgct aatggcaaaa ttgtcgcata tcttggcgat ccgatcacgg 6061 gactcgatct cccgtccggg cacaacggcc aacacctgta cgtaaaagtc cgccggattg 6121 tagttggtag gacactgggc acccacgctg gataggagtt gagatgttat gtaatactag 6181 atacccttaa taaacacatc gaactcacta ggaaaagaag tcgacggctt cgctgggagt 6241 gcccaagaaa gctaccctgc cctcggccat cagaaggatc ttgtcaaaga gctcaaacag 6301 ctcggaagac ggctgatgaa tggtcaggat gacggtcttg cccttctgcg acagcttctt 6361 cagcacctgg acgacgctgt gggcggtaaa ggagtccagt ccggaggtgg gctcatcgca 6421 gatcagaagc ggcggatcgg ttagagcctc ggaggcgaat gccagacgct tcctttctcc 6481 gccggacaga cctttcaccc tgccgggcac accgatgatc gtgtgctgac atttgctgag 6541 cgaaagctcc tggatcacct gatccacgcg ggccactcgc tgccgatagg tcagatgtcg 6601 tggcatccgc accatggctt ggaaaatcag gtgttccctg gccgttaggg agccgataaa 6661 gaggtcatcc tgctggacat aggcgcacct ggcctgcatc tccttggcgt ccacaggttg 6721 gccattgagc agtcgcatcc cggatggcga tacttggatg ccctgcggcg atcgaaaggc 6781 aagggcattc agcagggtcg tctttccggc accggaactg cccatcacgg ccaaaagttc 6841 gcccggatag gccacgccgc aaactgagtt tcaaattggt aattggaccc tttattaaga 6901 tttcacacag atcagccgac tgcgaataga aactcaccgt tcttgagcaa atgtttcctg 6961 ggcgccggta tgtgtcgctc gttgcagaat agtccgcgtg tccggttgac cagctgccgc 7021 catccggagc ccggctgatt gaccgcccca aagatgtcca tattgtgcca ggcataggtg 7081 aggttctcgg ctagttggcc gctccctgaa ccggagtcct ccggcggact gggtggccgg 7141 agcgtgccgt agtttttggc ctgcccgaag ccctggttaa tgcagctctg cgaagccgct 7201 ccgctgtcac cctgcaatga taggggatct caaatatcaa ctacaagcgt tatgctcatc 7261 taaccccgaa caaaaagtac cccgaagtat cctacgaagt aggtttatac ttttatttat 7321 tttttgtgca tctaggatca gcttaaaata tctggttgtt atattttttg taaaaaagaa 7381 tatagtcgaa aatgaatgcc tttagatgtc ttgatcatga tatgatctca aaaattgtct 7441 tatatagcga gaacagctac cagaataatc tgtttcgtgt cactatttgt ttgtgcaatt 7501 gcggtttggg atttttgtgg gtcgcagttc tcacgccgca gacaatttga tgttgcaatc 7561 gcagttccta tagatcaagt gaacttaaga tgtatgcaca tgtactactc acattgttca 7621 gatgctcggc agatgggtgt ttgctgcctc cgcgaattaa tagctcctga tcctcttggc 7681 ccattgccgg gatttttcac actttcccct gcttacccac ccaaaaccaa tcaccacccc 7741 aatcactcaa aaaacaaaca aaaataagaa gcgagaggag ttttggcaca gcactttgtg 7801 tttaattgat ggcgtaaacc gcttggagct tcgtcacgaa accgctgaca aaatgcaact 7861 gaaggcggac attgacgcta cgtaacgcta caaacggtgg cgaaagagat agcggacgca 7921 gcggcgaaag agacggcgat atttctgtgg acagagaagg aggcaaacag cgctgacttt 7981 gagtggaatg tcattttgag tgagaggtaa tcgaaagaac ctggtacatc aaataccctt 8041 ggatcgaagt aaatttaaaa ctgatcagat aagttcaatg atatccagtg cagtaaaaaa 8101 aaaaaatgtt ttttttatct actttccgca aaaatgggtt ttattaactt acatacatac 8161 taggggcccg gtacgtaccc aattcgccct atagtgagtc gtattacaat tcactggccg 8221 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag 8281 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc 8341 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg 8401 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc 8461 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa 8521 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac 8581 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt 8641 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca 8701 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt 8761 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta 8821 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 8881 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 8941 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc 9001 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga 9061 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct 9121 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg 9181 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta 9241 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat 9301 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt 9361 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga 9421 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga 9481 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga 9541 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc 9601 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 9661 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg 9721 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat 9781 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata 9841 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 9901 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 9961 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 10021 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 10081 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct 10141 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 10201 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 10261 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 10321 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 10381 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 10441 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 10501 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 10561 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 10621 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac 10681 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt 10741 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat 10801 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc 10861 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc 10921 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca 10981 tgattacgcc aagctcgaaa ttaaccctca ctaaagggaa caaaagctgg ctagaactag 11041 tgtcgacatg cccgccgtga ccgtcgagaa cccgctgacg ctgccccgcg tatccgcacc 11101 cgccgacgcc gtcgcacgtc ccgtgctcac cgtgaccacc gcgcccagcg gtttcgaggg 11161 cgagggcttc ccggtgcgcc gcgcgttcgc cgggatcaac taccgccacc tcgacccgtt 11221 catcatgatg gaccagatgg gtgaggtgga gtacgcgccc ggggagccca agggcacgcc 11281 ctggcacccg caccgcggct tcgagaccgt gacctacatc gtcgacacta gtggatctaa 11341 gcttaattga tcggctaaat ggtatggcaa gaaaaggtat gcaatataat aatcttttat 11401 tgggtatgca acgaaaattt gtttcgtcaa cgtatgcaat attttttatt aaaagagggt 11461 atgcaatgta ttttattaaa aacgggtatg caatataata atcttttatt gggtatgcaa 11521 cgaaaatttg tttcgtcaaa gtatgcaata ttttttatta aaagagggta tgcaatgtat 11581 tttattaaaa acgggtatgc aataaaaaat tatttggttt ctctaaaaag tatgcagcac 11641 ttattttttg ataaggtatg caacaaaatt ttactttgcc gaaaatatgc aatgtttttg 11701 cgaataaatt caacgcacac ttattacgtg aggtacctac tagtgccatt gctacaggca 11761 tcgttgcccg acattttgcc ctccggttcc caacgatcaa ctgcagaggc agcgactgcg 11821 ccgcgactgc ggagagaggg agagatacgg ttagccttct cgctcggatc gctctcgctt 11881 tacggcgcag ccattatggt gcgcgtagtc ttatctgtat ctcgctctta cgcacgtcag 11941 acttggaata gccctctctc tttttgagtt atcggcactt tggttctgcc gttatggcgg 12001 catcgtggtg tcacgctcta tataaacatg gaccgcagtt tgagctagtg ccattgctac 12061 aggcatcgtt gcccgacatt ttgccctccg gttcccaacg atcaactgca gaggcagcga 12121 ctgcgccgcg actgcggaga gagggagaga tacggttagc cttctcgctc ggatcgctct 12181 cgctttacgg cgcagccatt atggtgcgcg tagtcttatc tgtatctcgc tcttacgcac 12241 gtcagacttg gaatagccct ctctcttttt gagttatcgg cactttggtt ctgccgttat 12301 ggcggcatcg tggtgtcacg ctctatataa acatggaccg cagtttgagc tagtgccatt 12361 gctacaggca tcgttgcccg acattttgcc ctccggttcc caacgatcaa ctgcagaggc 12421 agcgactgcg ccgcgactgc ggagagaggg agagatacgg ttagccttct cgctcggatc 12481 gctctcgctt tacggcgcag ccattatggt gcgcgtagtc ttatctgtat ctcgctctta 12541 cgcacgtcag acttggaata gccctctctc tttttgagtt atcggcactt tggttctgcc 12601 gttatggcgg catcgtggtg tcacgctcta tataaacatg gaccgcagtt tgagctagtg 12661 ccattgctac aggcatcgtt gcccgacatt ttgccctccg gttcccaacg atcaactgca 12721 gaggcagcga ctgcgccgcg actgcggaga gagggagaga tacggttagc cttctcgctc 12781 ggatcgctct cgctttacgg cgcagccatt atggtgcgcg tagtcttatc tgtatctcgc 12841 tcttacgcac gtcagacttg gaatagccct ctctcttttt gagttatcgg cactttggtt 12901 ctgccgttat ggcggcatcg tggtgtcacg ctctatataa acatggaccg cagtttgagc 12961 tagtgccatt gctacaggca tcgttgcccg acattttgcc ctccggttcc caacgatcaa 13021 ctgcagaggc agcgactgcg ccgcgactgc ggagagaggg agagatacgg ttagccttct 13081 cgctcggatc gctctcgctt tacggcgcag ccattatggt gcgcgtagtc ttatctgtat 13141 ctcgctctta cgcacgtcag acttggaata gccctctctc tttttgagtt atcggcactt 13201 tggttctgcc gttatggcgg catcgtggtg tcacgctcta tataaacatg gaccgcagtt 13261 tgagctagtg ccattgctac aggcatcgtt gcccgacatt ttgccctccg gttcccaacg 13321 atcaactgca gaggcagcga ctgcgccgcg actgcggaga gagggagaga tacggttagc 13381 cttctcgctc ggatcgctct cgctttacgg cgcagccatt atggtgcgcg tagtcttatc 13441 tgtatctcgc tcttacgcac gtcagacttg gaatagccct ctctcttttt gagttatcgg 13501 cactttggtt ctgccgttat ggcggcatcg tggtgtcacg ctctatataa acatggaccg 13561 cagtttgagc tagtgccatt gctacaggca tcgttgcccg acattttgcc ctccggttcc 13621 caacgatcaa ctgcagaggc agcgactgcg ccgcgactgc ggagagaggg agagatacgg 13681 ttagccttct cgctcggatc gctctcgctt tacggcgcag ccattatggt gcgcgtagtc 13741 ttatctgtat ctcgctctta cgcacgtcag acttggaata gccctctctc tttttgagtt 13801 atcggcactt tggttctgcc gttatggcgg catcgtggtg tcacgctcta tataaacatg 13861 gaccgcagtt tgagctagtg ccattgctac aggcatcgtt gcccgacatt ttgccctccg 13921 gttcccaacg atcaactgca gaggcagcga ctgcgccgcg actgcggaga gagggagaga 13981 tacggttagc cttctcgctc ggatcgctct cgctttacgg cgcagccatt atggtgcgcg 14041 tagtcttatc tgtatctcgc tcttacgcac gtcagacttg gaatagccct ctctcttttt 14101 gagttatcgg cactttggtt ctgccgttat ggcggcatcg tggtgtcacg ctctatataa 14161 acatggaccg cagtttgagc tagcagatct ctagaatccc aaaacaaact ggttattgtg 14221 gtaggtcatt tgtttggcag aaagaaaact cgagaaattt ctctggccgt tattcgttat 14281 tctctctttt ctttttgggt ctctccctct ctgcactaat gctctctcac tctgtcacac 14341 agtaaacggc atactgctct cgttggttcg agagagcgcg cctcgaatgt tcgcgaaaag 14401 agcgccggag tataaataga ggcgcttcgt ctacggagcg acaattcaat tcaaacaagc 14461 aaagtgaaca cgtcgctaag cgaaagctaa gcaaataaac aagcgcagct gaacaagcta 14521 aacaatctgc agtaaagtgc aagttaaagt gaatcaatta aaagtaacca gcaaccaagt 14581 aaatcaactg caactactga aatctgccaa gaagtaatta ttgaatacaa gaagagaact 14641 ctg //