LOCUS       Exported                 861 bp ds-DNA     linear   SYN 10-JUN-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pB1H2w2-zif268
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Noyes MB, Meng X, Wakabayashi A, Sinha S, Brodsky MH, Wolfe SA
  TITLE     A systematic characterization of factors that regulate Drosophila 
            segmentation via a bacterial one-hybrid system.
  JOURNAL   Nucleic Acids Res. 2008 Mar 10. ():.
  PUBMED    18332042
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 10, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             217..486
                     /codon_start=1
                     /gene="E. coli rpoZ"
                     /product="DNA-directed RNA polymerase subunit omega"
                     /label=RNAP omega
                     /note="promotes RNA polymerase assembly"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDK
                     TTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGR"
     CDS             496..519
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 catncctacg cgcaatgcaa cctcccaatt caagtgcgga caacaaccct ttgcaacagc
       61 aatcatcaca aaataccgta ccaaacgtcc tcaaccaaat taaccaaatc ttttctccag
      121 aggagcaacg cagcttatta caagaagcca tcgaaacctg caagaatttt gaaaaaacac
      181 aattgtaagt ctcggctcca agaaggagat atacccatgg cacgcgtaac tgttcaggac
      241 gctgtagaga aaattggtaa ccgttttgac ctggtactgg tcgccgcgcg tcgcgctcgt
      301 cagatgcagg taggcggaaa ggacccgctc gtaccggaag aaaacgataa aaccactgta
      361 atcgcgctgc gcgaaatcga agaaggtctg atcaacaacc agatcctcga cgttcgcgaa
      421 cgccaggaac agcaagagca ggaagccgct gaattacaag ccgttaccgc tattgctgaa
      481 ggtcgtgcgg ccgcggacta caaggatgac gacgacaagt tccggaccgg ttccaagaca
      541 cccccccatg gtacccgccc atatgcttgc cctgtcgagt cctgcgatcg ccgcttttct
      601 cgctcggatg agcttaccgc ttcntntccg cntccatacc ggttcaaang ncccttccag
      661 tgtcgaatct gcatgcgtaa cttcagtcgt agtgaccacc ttaccaccca catccgcacc
      721 cacacaggcg agaagcnttt ngcctgtgac atttgtggga ggantttgcc aggngtgntg
      781 accncagagg ctancnaatc ntttttanaa aagangcntc taaaactaaa aaagccgaaa
      841 gtcccctcnc tgaaatctgg g
//
