LOCUS Exported 2294 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway entry 1A vector, no ccDB gene. ACCESSION . VERSION . KEYWORDS pENTR1A no ccDB (w48-1) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2294) AUTHORS Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD. TITLE A versatile viral system for expression and depletion of proteins in mammalian cells. JOURNAL PLoS One. 2009 Aug 6;4(8):e6529. PUBMED 19657394 REFERENCE 2 (bases 1 to 2294) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2294 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 103..189 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 281..308 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" primer_bind 316..339 /label=pENTR-F /note="pENTR vectors, forward primer" protein_bind 358..457 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(523..622) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(734..753) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 745..1554 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(812..831) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 1644..2232 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2133..2152 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg 61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct 121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt 181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg 241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat 301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa 361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc 421 ttttttataa tgccaacttt gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac 481 tggatccggt accgaattcg cggccgcact cgagatatct agacccagct ttcttgtaca 541 aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca 601 gtcaaaataa aatcattatt tgccatccag ctgcagctct ggcccgtgtc tcaaaatctc 661 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac 721 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga 781 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg 841 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg 901 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg 961 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca 1021 tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct 1081 gattcaggtg aaaatattgt tgatgcgctg gcagtgtccc tgcgccggtt gcattcgatt 1141 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca 1201 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct 1261 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc 1321 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt 1381 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac 1441 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat 1501 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa 1561 ttggttaatt ggttgtaaca ttattcagat tgggccccgt tccactgagc gtcagacccc 1621 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 1681 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 1741 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg 1801 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 1861 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 1921 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 1981 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 2041 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 2101 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 2161 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 2221 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 2281 tttgctcaca tgtt //