LOCUS       Exported               11109 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  PP7 coat protein-modified lentiviral packaging plasmid for packaging
            mRNA with an PP7 aptamer into lentivirus-like particles.
ACCESSION   .
VERSION     .
KEYWORDS    psPAX2-D64V-NC-PP7
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11109)
  AUTHORS   Lu B, Javidi-Parsijani P, Makani V, Mehraein-Ghomi F, Sarhan WM, Sun
            D, Yoo KW, Atala ZP, Lyu P, Atala A
  TITLE     Delivering SaCas9 mRNA by lentivirus-like bionanoparticles for 
            transient expression and efficient genome editing.
  JOURNAL   Nucleic Acids Res. 2019 Feb 13. pii: 5316732. doi: 
            10.1093/nar/gkz093.
  PUBMED    30759231
REFERENCE   2  (bases 1 to 11109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11109
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          663..1679
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1603..1625
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1687..1706
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3479..6490
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLVCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    6175..6292
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7064..7297
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7482..7526
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7820..7846
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     primer_bind     complement(8131..8150)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8196..8251
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8250..8269)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8612..8628)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8612..8628)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8625..8647)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8636..8652
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8660..8690)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8705..8726
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8785..8980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8831..8966
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8893..8912
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    8986..9120
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9023..9042)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9077..9096
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9188..9205)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9359..9947)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9439..9458)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10118..10978)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10741..10760
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10979..11083)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggnsgcg cgccrggsgg ggsggggsgg ggsgrggggs
      541 ggggsggggs grggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      661 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
      721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      781 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
      841 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
      961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     1021 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
     1081 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1261 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
     1321 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     1381 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     1441 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     1501 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     1561 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     1621 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     1681 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttcgggccgc
     1741 gttgacgcgc acggcaagag gcgaggggcg gcgactggtg agagatgggt gcgagagcgt
     1801 cagtattaag cgggggagaa ttagatcgat gggaaaaaat tcggttaagg ccagggggaa
     1861 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     1921 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     1981 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     2041 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     2101 aagagcaaaa caaaagtaag aaaaaagcac agcaagcagc agctgacaca ggacacagca
     2161 atcaggtcag ccaaaattac cctatagtgc agaacatcca ggggcaaatg gtacatcagg
     2221 ccatatcacc tagaacttta aatgcatggg taaaagtagt agaagagaag gctttcagcc
     2281 cagaagtgat acccatgttt tcagcattat cagaaggagc caccccacaa gatttaaaca
     2341 ccatgctaaa cacagtgggg ggacatcaag cagccatgca aatgttaaaa gagaccatca
     2401 atgaggaagc tgcagaatgg gatagagtgc atccagtgca tgcagggcct attgcaccag
     2461 gccagatgag agaaccaagg ggaagtgaca tagcaggaac tactagtacc cttcaggaac
     2521 aaataggatg gatgacacat aatccaccta tcccagtagg agaaatctat aaaagatgga
     2581 taatcctggg attaaataaa atagtaagaa tgtatagccc taccagcatt ctggacataa
     2641 gacaaggacc aaaggaaccc tttagagact atgtagaccg attctataaa actctaagag
     2701 ccgagcaagc ttcacaagag gtaaaaaatt ggatgacaga aaccttgttg gtccaaaatg
     2761 cgaacccaga ttgtaagact attttaaaag cattgggacc aggagcgaca ctagaagaaa
     2821 tgatgacagc atgtcaggga gtggggggac ccggccataa agcaagagtt ttggctgaag
     2881 caatgagcca agtaacaaat ccagctacca taatgataca gaaaggcaat tttaggaacc
     2941 aaagaaagac tgttaagtgt ttcaattgtg gcaaagaagg gcacatagcc aaaaattgca
     3001 gggcccctag gaaaaagggc tgttggaaat gtggaaagga aggacaccaa atgaaagatt
     3061 gttccggtgg aggtggatcc tccaaaacaa tagtcctctc cgtaggggag gcaacacgga
     3121 ctttgaccga aatccagtca accgctgacc gacaaatctt tgaagagaaa gtagggcctc
     3181 ttgtgggccg actgcgcttg actgcaagct tgcgacaaaa cggcgcaaag actgcctata
     3241 gggtcaacct taaactcgac caagccgacg tggtcgatag cggtctccct aaggttcggt
     3301 atacgcaggt ctggagtcat gacgtaacaa tcgtagcaaa cagcacagaa gcctcccgaa
     3361 aaagcctcta cgatctgacg aaatccttgg tggctacgtc acaggtggaa gacctcgttg
     3421 tcaaccttgt acctctgggt cggtccggag gtggaggtgg aactgagaga caggctaatt
     3481 ttttagggaa gatctggcct tcccacaagg gaaggccagg gaattttctt cagagcagac
     3541 cagagccaac agccccacca gaagagagct tcaggtttgg ggaagagaca acaactccct
     3601 ctcagaagca ggagccgata gacaaggaac tgtatccttt agcttccctc agatcactct
     3661 ttggcagcga cccctcgtca caataaagat aggggggcaa ttaaaggaag ctctattaga
     3721 tacaggagca gatgatacag tattagaaga aatgaatttg ccaggaagat ggaaaccaaa
     3781 aatgataggg ggaattggag gttttatcaa agtaagacag tatgatcaga tactcataga
     3841 aatctgcgga cataaagcta taggtacagt attagtagga cctacacctg tcaacataat
     3901 tggaagaaat ctgttgactc agattggctg cactttaaat tttcccatta gtcctattga
     3961 gactgtacca gtaaaattaa agccaggaat ggatggccca aaagttaaac aatggccatt
     4021 gacagaagaa aaaataaaag cattagtaga aatttgtaca gaaatggaaa aggaaggaaa
     4081 aatttcaaaa attgggcctg aaaatccata caatactcca gtatttgcca taaagaaaaa
     4141 agacagtact aaatggagaa aattagtaga tttcagagaa cttaataaga gaactcaaga
     4201 tttctgggaa gttcaattag gaataccaca tcctgcaggg ttaaaacaga aaaaatcagt
     4261 aacagtactg gatgtgggcg atgcatattt ttcagttccc ttagataaag acttcaggaa
     4321 gtatactgca tttaccatac ctagtataaa caatgagaca ccagggatta gatatcagta
     4381 caatgtgctt ccacagggat ggaaaggatc accagcaata ttccagtgta gcatgacaaa
     4441 aatcttagag ccttttagaa aacaaaatcc agacatagtc atctatcaat acatggatga
     4501 tttgtatgta ggatctgact tagaaatagg gcagcataga acaaaaatag aggaactgag
     4561 acaacatctg ttgaggtggg gatttaccac accagacaaa aaacatcaga aagaacctcc
     4621 attcctttgg atgggttatg aactccatcc tgataaatgg acagtacagc ctatagtgct
     4681 gccagaaaag gacagctgga ctgtcaatga catacagaaa ttagtgggaa aattgaattg
     4741 ggcaagtcag atttatgcag ggattaaagt aaggcaatta tgtaaacttc ttaggggaac
     4801 caaagcacta acagaagtag taccactaac agaagaagca gagctagaac tggcagaaaa
     4861 cagggagatt ctaaaagaac cggtacatgg agtgtattat gacccatcaa aagacttaat
     4921 agcagaaata cagaagcagg ggcaaggcca atggacatat caaatttatc aagagccatt
     4981 taaaaatctg aaaacaggaa agtatgcaag aatgaagggt gcccacacta atgatgtgaa
     5041 acaattaaca gaggcagtac aaaaaatagc cacagaaagc atagtaatat ggggaaagac
     5101 tcctaaattt aaattaccca tacaaaagga aacatgggaa gcatggtgga cagagtattg
     5161 gcaagccacc tggattcctg agtgggagtt tgtcaatacc cctcccttag tgaagttatg
     5221 gtaccagtta gagaaagaac ccataatagg agcagaaact ttctatgtag atggggcagc
     5281 caatagggaa actaaattag gaaaagcagg atatgtaact gacagaggaa gacaaaaagt
     5341 tgtcccccta acggacacaa caaatcagaa gactgagtta caagcaattc atctagcttt
     5401 gcaggattcg ggattagaag taaacatagt gacagactca caatatgcat tgggaatcat
     5461 tcaagcacaa ccagataaga gtgaatcaga gttagtcagt caaataatag agcagttaat
     5521 aaaaaaggaa aaagtctacc tggcatgggt accagcacac aaaggaattg gaggaaatga
     5581 acaagtagat aaattggtca gtgctggaat caggaaagta ctatttttag atggaataga
     5641 taaggcccaa gaagaacatg agaaatatca cagtaattgg agagcaatgg ctagtgattt
     5701 taacctacca cctgtagtag caaaagaaat agtagccagc tgtgataaat gtcagctaaa
     5761 aggggaagcc atgcatggac aagtagactg tagcccagga atatggcagc tagtttgtac
     5821 acatttagaa ggaaaagtta tcttggtagc agttcatgta gccagtggat atatagaagc
     5881 agaagtaatt ccagcagaga cagggcaaga aacagcatac ttcctcttaa aattagcagg
     5941 aagatggcca gtaaaaacag tacatacaga caatggcagc aatttcacca gtactacagt
     6001 taaggccgcc tgttggtggg cggggatcaa gcaggaattt ggcattccct acaatcccca
     6061 aagtcaagga gtaatagaat ctatgaataa agaattaaag aaaattatag gacaggtaag
     6121 agatcaggct gaacatctta agacagcagt acaaatggca gtattcatcc acaattttaa
     6181 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     6241 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     6301 ttacagggac agcagagatc cagtttggaa aggaccagca aagctcctct ggaaaggtga
     6361 aggggcagta gtaatacaag ataatagtga cataaaagta gtgccaagaa gaaaagcaaa
     6421 gatcatcagg gattatggaa aacagatggc aggtgatgat tgtgtggcaa gtagacagga
     6481 tgaggattaa cacatggaat tctgcaacaa ctgctgttta tccatttcag aattgggtgt
     6541 cgacatagca gaataggcgt tactcgacag aggagagcaa gaaatggagc cagtagatcc
     6601 tagactagag ccctggaagc atccaggaag tcagcctaaa actgcttgta ccaattgcta
     6661 ttgtaaaaag tgttgctttc attgccaagt ttgtttcatg acaaaagcct taggcatctc
     6721 ctatggcagg aagaagcgga gacagcgacg aagagctcat cagaacagtc agactcatca
     6781 agcttctcta tcaaagcagt aagtagtaca tgtaatgcaa cctataatag tagcaatagt
     6841 agcattagta gtagcaataa taatagcaat agttgtgtgg tccatagtaa tcatagaata
     6901 taggaaaatg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     6961 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     7021 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     7081 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     7141 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     7201 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     7261 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     7321 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     7381 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     7441 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     7501 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     7561 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     7621 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     7681 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     7741 acagagacag atccattcga ttagtgaacg gatccttggc acttatctgg gacgatctgc
     7801 ggagcctgtg cctcttcagc taccaccgct tgagagactt actcttgatt gtaacgagga
     7861 ttgtggaact tctgggacgc agggggtggg aagccctcaa atattggtgg aatctcctac
     7921 aatattggag tcaggagcta aagaatagtg ctgttagctt gctcaatgcc acagccatag
     7981 cagtagctga ggggacagat agggttatag aagtagtaca aggagcttgt agagctattc
     8041 gccacatacc tagaagaata agacagggct tggaaaggat tttgctataa gctcgaaaca
     8101 accggtacct ctagaactat agctagcaga tctttttccc tctgccaaaa attatgggga
     8161 catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc
     8221 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca
     8281 tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc
     8341 tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt
     8401 ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt
     8461 tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac tagccagatt
     8521 tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct
     8581 cgacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     8641 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     8701 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     8761 gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct
     8821 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     8881 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     8941 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca
     9001 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     9061 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     9121 cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     9181 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     9241 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     9301 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     9361 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     9421 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     9481 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     9541 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     9601 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     9661 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     9721 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     9781 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     9841 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     9901 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     9961 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    10021 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
    10081 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    10141 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    10201 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    10261 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    10321 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    10381 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    10441 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    10501 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    10561 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    10621 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    10681 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    10741 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    10801 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    10861 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    10921 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    10981 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    11041 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    11101 tgccacctg
//
