LOCUS Exported 823 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION Bacterial expression of mAmetrine (version 1.1) fluorescent protein. ACCESSION . VERSION . KEYWORDS pBad-mAmetrine1.1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 823) AUTHORS Ai HW, Hazelwood KL, Davidson MW, Campbell RE TITLE Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors. JOURNAL Nat Methods. 2008 May . 5(5):401-3. PUBMED 18425137 REFERENCE 2 (bases 1 to 823) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..823 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 85..102 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 106..138 /codon_start=1 /product="leader peptide from bacteriophage T7 gene 10" /label=T7 tag (gene 10 leader) /note="promotes efficient translation in E. coli" /translation="MASMTGGQQMG" CDS 142..165 /codon_start=1 /product="Xpress(TM) epitope tag, including an enterokinase recognition and cleavage site" /label=Xpress(TM) tag /translation="DLYDDDDK" primer_bind complement(226..247) /label=EGFP-N /note="EGFP, reverse primer" ORIGIN 1 ctactgagct tttttcgcaa ctctctactg tttctccata cccgtttttt gggctaacag 61 gaggaattaa ccatgggggg ttctcatcat catcatcatc atggtatggc tagcatgact 121 ggtggacagc aaatgggtcg ggatctgtac gacgatgacg ataaggatcc gagctcgagc 181 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 241 ggcgacgtaa acggccacag gttcagcgtg cgcggcgagg gcgagggcga tgccaccaac 301 ggcaagctga ccctgaagtt catctgcacc tccggcgagc tgcccgtgcc ctggcccacc 361 ctcgtgacca ccctgtctta cggcgtgcag tgcttcgccc gctaccccga ccacatgaag 421 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatctcc 481 ttcaaggacg acggcagcta caggacccgc gccgaggtga agttcgaggg cgacaccctg 541 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 601 aagctggagt acaacatgaa cgcgtgggac gcgtatatca cggccgacaa gcagaagaac 661 ggcatcaaag cgaacttcaa gatcgaccat anggggnngg nnngnggcct ggcagctcgc 721 cgacgcgtac cagcagaaca ccccatcggc gacggctcgt gctgctgctg acaccactac 781 ctgactncag acagtgtcaa aaccnaagac acgcgatact gtc //