LOCUS       Exported                8204 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA Myc DBC1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8204)
  AUTHORS   Hiraike H, Wada-Hiraike O, Nakagawa S, Koyama S, Miyamoto Y, Sone K,
            Tanikawa M, Tsuruga T, Nagasaka K, Matsumoto Y, Oda K, Shoji K, 
            Fukuhara H, Saji S, Nakagawa K, Kato S, Yano T, Taketani Y
  TITLE     Identification of DBC1 as a transcriptional repressor for BRCA1.
  JOURNAL   Br J Cancer. 2010 Mar 16;102(6):1061-7. Epub 2010 Feb 16.
  PUBMED    20160719
REFERENCE   2  (bases 1 to 8204)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8204
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(32..51)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        223..602
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        603..806
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     757..777
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     851..870
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        851..869
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      901..910
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             910..939
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(3745..3763)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3746..3763)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3783..3800)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3789..4013
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4059..4487
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4146..4165)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4356..4377
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4496..4516)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4501..4830
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4681..4816
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4743..4762
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4897..5691
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4951..4970)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5561..5580
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5865..5986
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5902..5921)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5956..5975
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6035..6051)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6035..6051)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6048..6070)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6059..6075
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6083..6113)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6128..6149
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6266..6283)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6437..7025)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6517..6536)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7196..8056)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7819..7838
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8057..8161)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
       61 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
      121 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
      181 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta
      241 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      301 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      361 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      421 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      481 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      541 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      601 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
      661 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      721 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
      781 ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact gcttactggc
      841 ttatcgaaat taatacgact cactataggg agacccaagc ttggtaccga gctcggatcc
      901 gccaccatgg aacagaagct catatctgaa gaggacctcg aattcatgtc ccagtttaag
      961 cgccagcgga tcaacccgct tccaggggga cgcaacttct caggcacagc ttcaacatct
     1021 cttctgggtc ctcctcctgg tttgctcact cctcctgtgg ccacagaact gtcccagaat
     1081 gccaggcacc ttcagggtgg ggagaaacag cgggtcttca ctggtattgt taccagcttg
     1141 catgactact ttggggttgt ggatgaagag gtcttttttc agctaagtgt ggtgaagggc
     1201 cgtctgcccc agctgggtga gaaggtgctg gtgaaggctg catacaaccc aggccaggca
     1261 gtgccctgga atgctgtcaa ggtgcaaacg ctctccaacc agcccctact gaagtcccca
     1321 gcacctcctc ttctgcatgt agcagccctg ggccagaagc aagggatcct gggagctcag
     1381 cctcagttga tcttccagcc tcaccggatt cccccactct ttcctcagaa gcctctgagt
     1441 ctcttccaaa catcccacac acttcacctg agccacctga acagatttcc tgcccggggc
     1501 cctcatggac ggttggatca gggccgaagt gatgactatg actccaagaa acgcaaacag
     1561 cgggctggtg gagagccctg gggtgctaag aagccaaggc atgacctgcc tccttaccgg
     1621 gtccacctca ctccttacac tgtggacagc cccatctgtg acttcctaga actccagcgc
     1681 cgttaccgca gcctcctggt cccctcagat tttctgtccg tgcatctgag ttggctatca
     1741 gccttccccc tgagccagcc cttttccctc catcatccaa gccggatcca ggtctcttct
     1801 gaaaaggagg cagctccaga cgctggtgct gagcccatca ctgcagacag tgaccccgct
     1861 tatagttcaa aggtactgct gctctcttcc ccggggttgg aggaattgta tcgttgttgc
     1921 atgctctttg tggatgacat ggctgagcca agggagacgc cagagcatcc tctgaagcag
     1981 attaagtttt tgctgggcag gaaagaagag gaggcagtgc tggttggggg tgaatggtct
     2041 ccttccctgg atggcctcga cccccaggct gacccgcagg tgctggtgcg taccgccatc
     2101 cgctgtgcgc aggcccagac tggcattgat ttgagcggct gtaccaagtg gtggcgcttt
     2161 gccgagtttc agtacctgca gccgggaccc ccccggcggc ttcagacagt ggtggtgtac
     2221 ctgccggatg tctggaccat catgcctact ttggaggagt gggaggccct gtgccagcag
     2281 aaagctgcag aggcagctcc cccaacccag gaggcacaag gggaaacgga gcctactgaa
     2341 caggcacctg atgccttgga gcaagcagca gacacttcta gacggaacgc agaaactcca
     2401 gaggccacca cacagcagga aacggacact gatctcccag aggcccctcc acccccccta
     2461 gaacctgctg tcatcgcacg ccctggctgt gtaaacctgt ccctccatgg gattgtggag
     2521 gatcggaggc caaaggaaag gatctctttt gaggtgatgg tgctggccga gctgtttctg
     2581 gagatgctcc agagggattt tggctataga gtttataaga tgctactgag ccttcctgaa
     2641 aaggtcgtgt ccccacctga acctgagaag gaggaggcgg ccaaggaaga agccaccaag
     2701 gaggaagaag ccatcaaaga ggaggtggtc aaggagccca aggatgaggc acagaatgag
     2761 ggcccggcta cagagtcaga ggccccgctg aaggaggatg ggcttttgcc caaaccactc
     2821 tcttctgggg gagaggaaga agaaaaaccc cggggcgagg cttctgagga cctgtgtgag
     2881 atggccctgg acccagaact gttgcttctg agggatgatg gagaggagga gtttgcagga
     2941 gcaaagctgg aggattcgga ggtccggtcc gttgcctcaa accagtcaga gatggagttc
     3001 tcttcacttc aggacatgcc caaggagctg gatccctctg ctgtgctccc cttagactgt
     3061 ctgcttgctt ttgtgttctt tgatgccaac tggtgtggct acttgcaccg gcgagactta
     3121 gagaggatcc tccttaccct tgggatccgg ctcagtgcag agcaggccaa gcagctggtc
     3181 agcagggtgg tgacccagaa catctgccag taccggagcc ttcagtacag ccgccaggag
     3241 ggcctggatg gtggccttcc cgaggaggtg ctcttcggaa acctggacct gctgccccct
     3301 cctgggaaaa gcacgaagcc aggtgccgcc cccacagaac acaaagcctt ggtgtcccac
     3361 aatggcagcc tgattaacgt ggggagcctg ctgcagcgcg cggagcagca ggacagcggc
     3421 cggctctacc tagagaacaa gatccacaca ctggagctga agctggagga gagccataac
     3481 cgtttctcag ccagtgaagt aaccaataag acgctggcgg cagagatgca ggagctgcga
     3541 gtccggctgg cggaggccga ggagaccgcc cggacggcgg agcgacagaa gagccagctc
     3601 cagcggctgc tgcaggagct ccgcaggcgt ctgacccccc tgcagctgga gatccagcgg
     3661 gtggtggaaa aggctgacag ctgggtggag aaggaggagc cggcacctag caactgactc
     3721 gagcatgcat ctagagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc
     3781 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     3841 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     3901 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     3961 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     4021 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     4081 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     4141 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     4201 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     4261 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     4321 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     4381 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     4441 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     4501 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4561 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     4621 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     4681 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     4741 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     4801 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     4861 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     4921 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     4981 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     5041 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
     5101 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     5161 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     5221 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     5281 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     5341 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     5401 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
     5461 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     5521 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     5581 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     5641 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     5701 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     5761 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     5821 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     5881 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     5941 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     6001 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     6061 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     6121 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     6181 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     6241 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     6301 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     6361 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     6421 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     6481 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     6541 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     6601 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     6661 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     6721 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     6781 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     6841 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     6901 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     6961 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     7021 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     7081 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     7141 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     7201 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     7261 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     7321 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     7381 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     7441 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     7501 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     7561 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     7621 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     7681 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     7741 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     7801 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     7861 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     7921 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     7981 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     8041 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     8101 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     8161 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc ggga
//
