LOCUS Exported 7323 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION ""Activate neurons with 2p stimulation"". ACCESSION . VERSION . KEYWORDS pAAV-CamKIIa-C1V1::FusionRed::Kv2.1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7323) TITLE AAV plasmids expressing Red-shifted channelrhodopsins with soma localization sequence JOURNAL Unpublished REFERENCE 2 (bases 1 to 7323) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7323 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 51..180 /label=AAV2 ITR (alternate) /note="Functional equivalent of wild-type AAV2 ITR" promoter 199..1487 /label=CaMKII promoter /note="promoter for the mouse calcium/calmodulin-dependent kinase II alpha subunit" misc_feature 3500..4088 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(3553..3573) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(3971..3982) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 4120..4596 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(4319..4338) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" repeat_region 4636..4776 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 4636..4765 /label=AAV2 ITR rep_origin 4851..5306 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4938..4957) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5148..5169 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(5323..5342) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 5442..5464 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(5502..5520) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 5588..5692 /gene="bla" /label=AmpR promoter CDS 5693..6553 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5911..5930) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6724..7312 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7213..7232 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt cctgcaggca 61 gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt ggtcgcccgg 121 cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact aggggttcct 181 gcggccgcac gcgtttaaca ttatggcctt aggtcacttc atctccatgg ggttcttctt 241 ctgattttct agaaaatgag atgggggtgc agagagcttc ctcagtgacc tgcccagggt 301 cacatcagaa atgtcagagc tagaacttga actcagatta ctaatcttaa attccatgcc 361 ttgggggcat gcaagtacga tatacagaag gagtgaactc attagggcag atgaccaatg 421 agtttaggaa agaagagtcc agggcagggt acatctacac cacccgccca gccctgggtg 481 agtccagcca cgttcacctc attatagttg cctctctcca gtcctacctt gacgggaagc 541 acaagcagaa actgggacag gagccccagg agaccaaatc ttcatggtcc ctctgggagg 601 atgggtgggg agagctgtgg cagaggcctc aggaggggcc ctgctgctca gtggtgacag 661 ataggggtga gaaagcagac agagtcattc cgtcagcatt ctgggtctgt ttggtacttc 721 ttctcacgct aaggtggcgg tgtgatatgc acaatggcta aaaagcaggg agagctggaa 781 agaaacaagg acagagacag aggccaagtc aaccagacca attcccagag gaagcaaaga 841 aaccattaca gagactacaa gggggaaggg aaggagagat gaattagctt cccctgtaaa 901 ccttagaacc cagctgttgc cagggcaacg gggcaatacc tgtctcttca gaggagatga 961 agttgccagg gtaactacat cctgtctttc tcaaggacca tcccagaatg tggcacccac 1021 tagccgttac catagcaact gcctctttgc cccacttaat cccatcccgt ctgttaaaag 1081 ggccctatag ttggaggtgg gggaggtagg aagagcgatg atcacttgtg gactaagttt 1141 gttcgcatcc ccttctccaa ccccctcagt acatcaccct gggggaacag ggtccacttg 1201 ctcctgggcc cacacagtcc tgcagtattg tgtatataag gccagggcaa agaggagcag 1261 gttttaaagt gaaaggcagg caggtgttgg ggaggcagtt accggggcaa cgggaacagg 1321 gcgtttcgga ggtggttgcc atggggacct ggatgctgac gaaggctcgc gaggctgtga 1381 gcagccacag tgccctgctc agaagcccca agctcgtcag tcaagccggt tctccgtttg 1441 cactcaggag cacgggcagg cgagtggccc ctagttctgg gggcagctct agagcggtac 1501 cggatccccg ggtaccggtg ccaccatgag tcggcgaccc tggcttctcg cacttgcttt 1561 ggctgtagct ttggcagctg gaagtgctgg ggctagtacc gggtctgacg ctactgtccc 1621 tgtggccacc caggacggac cagattatgt cttccataga gcccacgaga gaatgctttt 1681 ccagactagc tatacccttg aaaataacgg tagcgtaatt tgcattccca ataacggcca 1741 gtgtttctgc ctggcttggt tgaagtctaa cggcacaaac gccgaaaagc tggctgcaaa 1801 tatacttcaa tggattacct tcgcattgag tgcattgtgt ctcatgttct atggttacca 1861 aacctggaag agcacttgcg ggtgggagac tatctacgtt gctacaatag aaatgataaa 1921 gttcataatt gaatacttcc atgaatttga tgaacctgcc gtcatttaca gttcaaatgg 1981 taataaaacc gtttggttga gatacgcaac atggctgctg acttgccccg tcttgcttat 2041 tcatctgagt aatctgactg gtctgaagga cgactattct aaacgaacaa tggggctcct 2101 cgtcagtgat gtgggctgta tcgtctgggg cgcaaccagc gctatgtgta ccgggtggac 2161 aaaaatactg ttcttcctta ttagcctgtc ttatgggatg tacacctact tccacgctgc 2221 taaagtctat atcgaagcct ttcacactgt ccccaagggc atatgtcggg aactggtaag 2281 ggtgatggct tggaccttct ttgttgcttg gggcatgttc cctgttttgt ttctgttggg 2341 caccgaggga tttggtcata tttctccata tggaagcgct atcggccatt caatccttga 2401 tctcatagct aaaaacatgt ggggtgtgct ggggaattac ttgcgagtta aaattcatga 2461 gcatattctt ctttatggcg acataagaaa aaaacaaaag atcactattg cagggcaaga 2521 aatggaggtc gaaacccttg ttgcagagga ggaggacgcc gctccagtag tagctgtgag 2581 cgaactcata aaggagaata tgcctatgaa actttacatg gaagggaccg taaacaatca 2641 tcacttcaaa tgtacttccg aaggtgaggg taaaccttat gaaggcacac aaacaatgag 2701 gattaaggtg gttgaaggtg gccccttgcc attcgctttt gacattttgg caacaagctt 2761 catgtacggc tcacgaacat ttattaaaca cccccccggt attcctgact tctttaagca 2821 aagcttccct gagggtttta catgggaacg ggtaaccacc tacgaggacg gcggcgttct 2881 gacagcaaca caagacacaa gtctgcagga cgggtgtctc atctacaatg tgaaagtaag 2941 aggtgtcaat ttccccgcta acggtcctgt aatgcaaaag aaaacactcg ggtgggaggc 3001 ttccacagaa acaatgtatc cagccgacgg aggcttggag ggggcctgcg acatggcctt 3061 gaagcttgta gggggagggc atctcatatg taacctcgag acaacttaca ggagcaagaa 3121 acccgccact aatttgaaga tgcccggggt ctacaacgtt gatcaccggc ttgagcgaat 3181 caaagaggct gacgacgaaa cctatgtaga acaacacgaa gttgccgtgg cccgatatag 3241 caccgggggt gccggtgatg gtgggaagta caaacaaagc cagcccatcc ttaacactaa 3301 agagatggct ccacaatcaa aaccacccga ggagcttgaa atgagctcca tgccttctcc 3361 tgtagctccc ctccccgcaa gaacagaagg ggtgattgac atgcgctcta tgagcagtat 3421 agacagcttc atctcatgtg ctaccgactt cccagaagca acaaggttta agtaagaatt 3481 cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg 3541 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta 3601 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct 3661 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt 3721 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccggaac 3781 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg 3841 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc 3901 gtcctttcct tggctgctcg cctatgttgc cacctggatt ctgcgcggga cgtccttctg 3961 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct 4021 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc 4081 ctccccgcat cgataccgag cgctgctcga gagatctacg ggtggcatcc ctgtgacccc 4141 tccccagtgc ctctcctggc cctggaagtt gccactccag tgcccaccag ccttgtccta 4201 ataaaattaa gttgcatcat tttgtctgac taggtgtcct tctataatat tatggggtgg 4261 aggggggtgg tatggagcaa ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc 4321 tattgggaac caagctggag tgcagtggca caatcttggc tcactgcaat ctccgcctcc 4381 tgggttcaag cgattctcct gcctcagcct cccgagttgt tgggattcca ggcatgcatg 4441 accaggctca gctaattttt gtttttttgg tagagacggg gtttcaccat attggccagg 4501 ctggtctcca actcctaatc tcaggtgatc tacccacctt ggcctcccaa attgctggga 4561 ttacaggcgt gaaccactgc tcccttccct gtccttctga ttttgtaggt aaccacgtgc 4621 ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc 4681 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg 4741 cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc 4801 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg 4861 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc 4921 cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg 4981 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg 5041 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg 5101 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt 5161 ccaaactgga acaacactca actctatctc gggctattct tttgatttat aagggatttt 5221 gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt 5281 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc 5341 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg 5401 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca 5461 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt 5521 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg 5581 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct 5641 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat 5701 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc 5761 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg 5821 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg 5881 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga 5941 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta 6001 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc 6061 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc 6121 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg 6181 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc 6241 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca 6301 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct 6361 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat 6421 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg 6481 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat 6541 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact 6601 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 6661 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 6721 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 6781 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 6841 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca 6901 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 6961 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 7021 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 7081 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 7141 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 7201 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 7261 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 7321 caa //