LOCUS       Exported                9934 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  piggyBac-based expression of the tamoxifen-inducible Cre.
ACCESSION   .
VERSION     .
KEYWORDS    KA1158_pPBCAG-MerCreMer-IZ
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9934)
  AUTHORS   Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, 
            Arauzo-Bravo MJ, Boerno ST, Timmermann B, Vingron M, Scholer HR
  TITLE     Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive 
            Pluripotency.
  JOURNAL   Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 
            10.1016/j.stem.2018.05.020. Epub 2018 Jun 14.
  PUBMED    29910149
REFERENCE   2  (bases 1 to 9934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9934
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        221..524
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        529..806
                     /label=chicken beta-actin promoter
     intron          807..1823
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1747..1769
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1831..1850
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2906..2926
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2927..3952
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     misc_feature    4988..5574
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5155..5172)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5382..5401
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5575..5949
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     primer_bind     complement(6007..6026)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6072..6127
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6126..6145)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6488..6504)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6512..6528
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6536..6566)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6581..6602
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   6829..6891
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(6915..6933)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6954..6970)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6978..6994
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7002..7032)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7047..7068
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7185..7202)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7356..7944)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7436..7455)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8115..8975)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8738..8757
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8976..9080)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9106..9561)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9243..9264)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9455..9474
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9688..9710
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9702..9719
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9703..9719
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9726..9745
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9726..9744
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   9763..9797
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 gaccgcgtga gtcaaaatga cgcatgatta tcttttacgt gacttttaag atttaactca
       61 tacgataatt atattgttat ttcatgttct acttacgtga taacttatta tatatatatt
      121 ttcttgttat agatatcgtc gacattgatt attgactagc tagttattaa tagtaatcaa
      181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      301 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      481 ctacttggca gtacatctac gtattagtca tcgctattac catgatggtc gaggtgagcc
      541 ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat
      601 ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccrg
      661 gsggggsggg gsggggsgrg gggsggggsg gggcgaggcg gagaggtgcg gcggcagcca
      721 atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct
      781 ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc
      841 gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg
      901 tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc
      961 ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg
     1021 gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct
     1081 ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca
     1141 gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg
     1201 ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt
     1261 cggtcgggct gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt
     1321 cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc
     1381 ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc cggggrgggc tcgggggagg
     1441 ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc
     1501 ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc
     1561 cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg
     1621 ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc
     1681 tccctctcca gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca
     1741 gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc
     1801 atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc
     1861 attttggcaa agaattcctc gagatctcac gcgtgactag cttgcatgcc tgcacaccat
     1921 gggagatcca cgaaatgaaa tgggtgcttc aggagacatg agggctgcca acctttggcc
     1981 aagccctctt gtgattaagc acactaagaa gaatagccct gccttgtcct tgacagctga
     2041 ccagatggtc agtgccttgt tggatgctga accgcccatg atctattctg aatatgatcc
     2101 ttctagaccc ttcagtgaag cctcaatgat gggcttattg accaacctag cagataggga
     2161 gctggttcat atgatcaact gggcaaagag agtgccaggc tttggggact tgaatctcca
     2221 tgatcaggtc caccttctcg agtgtgcctg gctggagatt ctgatgattg gtctcgtctg
     2281 gcgctccatg gaacacccgg ggaagctcct gtttgctcct aacttgctcc tggacaggaa
     2341 tcaaggtaaa tgtgtggaag gcatggtgga gatctttgac atgttgcttg ctacgtcaag
     2401 tcggttccgc atgatgaacc tgcagggtga agagtttgtg tgcctcaaat ccatcatttt
     2461 gcttaattcc ggagtgtaca cgtttctgtc cagcaccttg aagtctctgg aagagaagga
     2521 ccacatccac cgtgtcctgg acaagatcac agacactttg atccacctga tggccaaagc
     2581 tggcctgact ctgcagcagc agcatcgccg cctagctcag ctccttctca ttctttccca
     2641 tatccggcat atgagtaaca aacgcatgga gcatctctac aacatgaaat gcaagaacgt
     2701 ggtacccctc tatgacctgc tcctggagat gttggatgcc caccgccttc atgccccagc
     2761 cagtcgcatg ggagtgcccc cagaggagcc cagccagacc cagctggcca ccaccagctc
     2821 cacttcagca cattccttac aaacctacta catacccccg gaagcagagg gcttccccaa
     2881 cacgatcgtc gacctgcaga ccatgcccaa gaagaagagg aaggtgtcca atttactgac
     2941 cgtacaccaa aatttgcctg cattaccggt cgatgcaacg agtgatgagg ttcgcaagaa
     3001 cctgatggac atgttcaggg atcgccaggc gttttctgag catacctgga aaatgcttct
     3061 gtccgtttgc cggtcgtggg cggcatggtg caagttgaat aaccggaaat ggtttcccgc
     3121 agaacctgaa gatgttcgcg attatcttct atatcttcag gcgcgcggtc tggcagtaaa
     3181 aactatccag caacatttgg gccagctaaa catgcttcat cgtcggtccg ggctgccacg
     3241 accaagtgac agcaatgctg tttcactggt tatgcggcgg atccgaaaag aaaacgttga
     3301 tgccggtgaa cgtgcaaaac aggctctagc gttcgaacgc actgatttcg accaggttcg
     3361 ttcactcatg gaaaatagcg atcgctgcca ggatatacgt aatctggcat ttctggggat
     3421 tgcttataac accctgttac gtatagccga aattgccagg atcagggtta aagatatctc
     3481 acgtactgac ggtgggagaa tgttaatcca tattggcaga acgaaaacgc tggttagcac
     3541 cgcaggtgta gagaaggcac ttagcctggg ggtaactaaa ctggtcgagc gatggatttc
     3601 cgtctctggt gtagctgatg atccgaataa ctacctgttt tgccgggtca gaaaaaatgg
     3661 tgttgccgcg ccatctgcca ccagccagct atcaactcgc gccctggaag ggatttttga
     3721 agcaactcat cgattgattt acggcgctaa ggatgactct ggtcagagat acctggcctg
     3781 gtctggacac agtgcccgtg tcggagccgc gcgagatatg gcccgcgctg gagtttcaat
     3841 accggagatc atgcaagctg gtggctggac caatgtaaat attgtcatga actatatccg
     3901 taacctggat agtgaaacag gggcaatggt gcgcctgctg gaagatggcg atggaggttc
     3961 tggagatcca cgaaatgaaa tgggtgcttc aggagacatg agggctgcca acctttggcc
     4021 aagccctctt gtgattaagc acactaagaa gaatagccct gccttgtcct tgacagctga
     4081 ccagatggtc agtgccttgt tggatgctga accgcccatg atctattctg aatatgatcc
     4141 ttctagaccc ttcagtgaag cctcaatgat gggcttattg accaacctag cagataggga
     4201 gctggttcat atgatcaact gggcaaagag agtgccaggc tttggggact tgaatctcca
     4261 tgatcaggtc caccttctcg agtgtgcctg gctggagatt ctgatgattg gtctcgtctg
     4321 gcgctccatg gaacacccgg ggaagctcct gtttgctcct aacttgctcc tggacaggaa
     4381 tcaaggtaaa tgtgtggaag gcatggtgga gatctttgac atgttgcttg ctacgtcaag
     4441 tcggttccgc atgatgaacc tgcagggtga agagtttgtg tgcctcaaat ccatcatttt
     4501 gcttaattcc ggagtgtaca cgtttctgtc cagcaccttg aagtctctgg aagagaagga
     4561 ccacatccac cgtgtcctgg acaagatcac agacactttg atccacctga tggccaaagc
     4621 tggcctgact ctgcagcagc agcatcgccg cctagctcag ctccttctca ttctttccca
     4681 tatccggcat atgagtaaca aacgcatgga gcatctctac aacatgaaat gcaaaaacgt
     4741 ggtacccctc tatgacctgc tcctggagat gttggatgcc caccgccttc atgccccagc
     4801 cagtcgcatg ggagtgcccc cagaggagcc cagccagacc cagctggcca ccaccagctc
     4861 cacttcagca cattccttac aaacctacta catacccccg gaagcagagg gcttccccaa
     4921 cacgatctga gagctcctac tagctgagcg gccgctcgat aagcttgata tcgaattccg
     4981 cccccccccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
     5041 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     5101 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     5161 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     5221 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     5281 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     5341 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     5401 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     5461 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     5521 accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac aaccatggcc
     5581 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc
     5641 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc
     5701 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc
     5761 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg
     5821 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg
     5881 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag
     5941 caggactgac cgacgccgac caacaccgcc ggtccgacgc ggcccgacgg gtccgagggg
     6001 ggtcgatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct
     6061 gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg
     6121 tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt
     6181 ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata
     6241 aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc
     6301 cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc
     6361 cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc
     6421 agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca agcttggcgt
     6481 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     6541 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     6601 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc
     6661 tttgttactt tatagaagaa attttgagtt tttgtttttt tttaataaat aaataaacat
     6721 aaataaattg tttgttgaat ttattattag tatgtaagtg taaatataat aaaacttaat
     6781 atctattcaa attaataaat aaacctcgat atacagaccg ataaaacaca tgcgtcaatt
     6841 ttacgcatga ttatctttaa cgtacgtcac aatatgatta tctttctagg gttaaagagc
     6901 tccagctttt gttcccttta gtgagggtta atttcgagct tggcgtaatc atggtcatag
     6961 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     7021 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     7081 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     7141 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     7201 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7261 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     7321 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7381 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7441 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7501 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7561 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7621 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7681 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7741 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     7801 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7861 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7921 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7981 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     8041 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     8101 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     8161 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     8221 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     8281 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     8341 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     8401 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     8461 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     8521 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     8581 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     8641 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     8701 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     8761 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8821 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     8881 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     8941 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     9001 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     9061 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta
     9121 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     9181 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     9241 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     9301 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     9361 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     9421 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     9481 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     9541 atgcgccgct acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc
     9601 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     9661 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     9721 aattgtaata cgactcacta tagggcgaat tgggtacctt aaccctagaa agatagtctg
     9781 cgtaaaattg acgcatgcat tcttgaaata ttgctctctc tttctaaata gcgcgaatcc
     9841 gtcgctgtgc atttaggaca tctcagtcgc cgcttggagc tcccgtgagg cgtgcttgtc
     9901 aatgcggtaa gtgtcactga ttttgaacta taac
//
