LOCUS       Exported                8059 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  piggyBac-based Dox-inducible expression of FLAG-Esrrb.
ACCESSION   .
VERSION     .
KEYWORDS    KA2880_pPB-TREtight-FLAG-Esrrb-InO
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8059)
  AUTHORS   Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, 
            Arauzo-Bravo MJ, Boerno ST, Timmermann B, Vingron M, Scholer HR
  TITLE     Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive 
            Pluripotency.
  JOURNAL   Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 
            10.1016/j.stem.2018.05.020. Epub 2018 Jun 14.
  PUBMED    29910149
REFERENCE   2  (bases 1 to 8059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8059
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        179..493
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    187..205
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    223..241
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    258..276
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    294..312
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    330..348
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    365..383
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    401..419
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     466..490
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          537..1109
                     /label=-beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             1194..1259
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    2629..3215
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2796..2813)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3023..3042
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3216..3593
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSAK"
     regulatory      3606..3615
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3612..4265
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     primer_bind     complement(4302..4319)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4308..4532
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4579..4595)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4603..4619
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4627..4657)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4672..4693
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   4920..4982
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(5006..5024)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5045..5061)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5069..5085
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5093..5123)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5138..5159
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5276..5293)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5447..6035)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5527..5546)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6206..7066)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6829..6848
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7067..7171)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7197..7652)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7334..7355)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7546..7565
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7779..7801
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7793..7810
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7794..7810
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7817..7836
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7817..7835
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   7854..7888
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 ttacgtgact tttaagattt aactcatacg ataattatat tgttatttca tgttctactt
       61 acgtgataac ttattatata tatattttct tgttatagat atcataattt aaacaagcaa
      121 aaccaaatta agggccagct cattcctccc actcatgatc tatagatctg gatccgtcga
      181 gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
      241 aacgatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt tactccctat
      301 cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag
      361 tttatcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      421 cgtatgtcga ggtaggcgtg tacggtggga ggcctatata agcagagctc gtttagtgaa
      481 ccgtcagatc gcctggagaa ttcgagctcg gtacccggga tcctgagaac ttcagggtga
      541 gtttggggac ccttgattgt tctttctttt tcgctattgt aaaattcatg ttatatggag
      601 ggggcaaagt tttcagggtg ttgtttagaa tgggaagatg tcccttgtat caccatggac
      661 cctcatgata attttgtttc tttcactttc tactctgttg acaaccattg tctcctctta
      721 ttttcttttc attttctgta actttttcgt taaactttag cttgcatttg taacgaattt
      781 ttaaattcac ttttgtttat ttgtcagatt gtaagtactt tctctaatca cttttttttc
      841 aaggcaatca gggtatatta tattgtactt cagcacagtt ttagagaaca attgttataa
      901 ttaaatgata aggtagaata tttctgcata taaattctgg ctggcgtgga aatattctta
      961 ttggtagaaa caactacacc ctggtcatca tcctgccttt ctctttatgg ttacaatgat
     1021 atacactgtt tgagatgagg ataaaatact ctgagtccaa accgggcccc tctgctaacc
     1081 atgttcatgc cttcttctct ttcctacagc tcctgggcaa cgtgctggtt gttgtgctgt
     1141 ctcatcattt tggcaaagaa ttcctcgaga tctcacgcgt gactagtgcc atggactaca
     1201 aggaccacga cggcgactac aaggaccacg acatcgacta caaggacgac gacgacaagg
     1261 gctccggagt cgagaccacc atgtcgtccg aagacaggca cctgggctct agttgcggct
     1321 ccttcatcaa gacggagcca tccagcccgt cctcgggcat tgatgccctc agccaccaca
     1381 gccccagcgg ctcgtcggac gccagtggtg gctttggcat tgccctgagc acccacgcca
     1441 acggtctgga ctcgccgcct atgttcgcag gtgcggggct gggaggcaac ccgtgccgca
     1501 agagctacga ggactgtact agtggtatca tggaggactc cgccatcaaa tgcgagtaca
     1561 tgcttaacgc catccccaag cgcctgtgcc tcgtgtgcgg ggacattgcc tctggctacc
     1621 actacggagt ggcctcctgc gaggcttgca aggcgttctt caagagaacc attcaaggca
     1681 acatcgagta caactgcccg gccaccaatg aatgtgagat caccaaacgg aggcgcaagt
     1741 cctgtcaggc ctgccgattc atgaaatgcc tcaaagtggg gatgctgaag gaaggtgtgc
     1801 gccttgaccg agttcgagga ggccgccaga agtacaagcg acggctggat tcggagaaca
     1861 gcccctacct gaacctgccg atttccccac ctgctaaaaa gccattgact aagatcgtct
     1921 cgaatctact aggggttgag caggacaagc tgtatgctat gcctcccaac gatatccccg
     1981 agggagatat caaggccctg accactctct gtgaattggc agatcgggag cttgtgttcc
     2041 tcatcaactg ggccaagcac atcccaggct tccccagtct gacacttggg gaccagatga
     2101 gcctgctgca gagtgcctgg atggagattc tcatcttggg catcgtgtac cgctcgctcc
     2161 catacgatga caagctggca tacgccgagg actatatcat ggatgaggaa cactctcgcc
     2221 tggtagggct gctggacctt taccgagcca tcctgcagct ggtgcgcagg tacaagaaac
     2281 tcaaggtaga gaaggaagag tttatgatcc tcaaggccct ggccctcgcc aactcagatt
     2341 cgatgtacat tgagaacctg gaggcggtgc agaagctcca ggacctgctg cacgaggcgc
     2401 tgcaggacta tgagctgagt cagcgccacg aggagccgcg gagggccggc aagctgctgc
     2461 tgacgctgcc cctgctgagg cagacagccg ccaaagccgt gcaacacttc tacagtgtga
     2521 aactgcaggg caaggtgccc atgcacaaac tcttcctgga gatgctggag gccaaggtgt
     2581 ctagatgagc ggccgctcga taagcttgat atcgaattcc gccccccccc cctctccctc
     2641 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     2701 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     2761 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     2821 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     2881 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     2941 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     3001 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     3061 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
     3121 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
     3181 cctttgaaaa acacgatgat aatatggcca caaccatgcc agagccagcg aagtctgctc
     3241 ccgccccgaa aaagggctcc aagaaggcgg tgactaaggc gcagaagaaa gacggcaaga
     3301 agcgcaagcg cagccgcaag gagagctatt ccatctatgt gtacaaggtt ctgaagcagg
     3361 tccaccctga caccggcatt tcgtccaagg ccatgggcat catgaattcg tttgtgaacg
     3421 acattttcga gcgcatcgca ggtgaggctt cccgcctggc gcattacaac aagcgctcga
     3481 ccatcacctc cagggagatc cagacggccg tgcgcctgct gctgcctggg gagttggcca
     3541 agcacgccgt gtccgagggt actaaggccg tcaccaagta caccagcgct aagctcgaga
     3601 ctagtgccac catggtgagt gtgattaaac cagagatgaa gatgaggtac tacatggacg
     3661 gctccgtcaa tgggcatgag ttcacaattg aaggtgaagg cacaggcaga ccttacgagg
     3721 gacatcaaga gatgacacta cgcgtcacaa tggccgaggg cgggccaatg cctttcgcgt
     3781 ttgacttagt gtcacacgtg ttctgttacg gccacagagt atttactaaa tatccagaag
     3841 agataccaga ctatttcaaa caagcatttc ctgaaggcct gtcatgggaa aggtcgttgg
     3901 agttcgaaga tggtgggtcc gcttcagtca gtgcgcatat aagccttaga ggaaacacct
     3961 tctaccacaa atccaaattt actggggtta actttcctgc cgatggtcct atcatgcaaa
     4021 accaaagtgt tgattgggag ccatcaaccg agaaaattac tgccagcgac ggagttctga
     4081 agggtgatgt tacgatgtac ctaaaacttg aaggaggcgg caatcacaaa tgccaaatga
     4141 agactactta caaggcggca aaagagattc ttgaaatgcc aggagaccat tacatcggcc
     4201 atcgcctcgt caggaaaacc gaaggcaaca ttactgagca ggtagaagat gcagtagctc
     4261 attccgctag ctgaatcgat gatctagagc tcgctgatca gcctcgactg tgccttctag
     4321 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     4381 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     4441 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     4501 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa cctgcagccc
     4561 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     4621 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     4681 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     4741 ccagcggatc ctttgttact ttatagaaga aattttgagt ttttgttttt ttttaataaa
     4801 taaataaaca taaataaatt gtttgttgaa tttattatta gtatgtaagt gtaaatataa
     4861 taaaacttaa tatctattca aattaataaa taaacctcga tatacagacc gataaaacac
     4921 atgcgtcaat tttacgcatg attatcttta acgtacgtca caatatgatt atctttctag
     4981 ggttaaagag ctccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat
     5041 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     5101 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     5161 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     5221 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     5281 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     5341 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     5401 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     5461 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     5521 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     5581 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     5641 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     5701 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     5761 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     5821 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     5881 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     5941 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     6001 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     6061 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     6121 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     6181 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     6241 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     6301 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     6361 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     6421 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     6481 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     6541 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     6601 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     6661 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     6721 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     6781 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     6841 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     6901 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     6961 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacggga aggcaaaatg
     7021 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     7081 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     7141 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat
     7201 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     7261 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     7321 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     7381 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     7441 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     7501 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     7561 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     7621 cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg
     7681 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     7741 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     7801 gacggccagt gaattgtaat acgactcact atagggcgaa ttgggtacct taaccctaga
     7861 aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat attgctctct ctttctaaat
     7921 agcgcgaatc cgtcgctgtg catttaggac atctcagtcg ccgcttggag ctcccgtgag
     7981 gcgtgcttgt caatgaggta agtgtcactg attttgaact ataacgaccg cgtgagtcaa
     8041 aatgacgcat gattatctt
//
