LOCUS Exported 11696 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral expression of PIK3CA. ACCESSION . VERSION . KEYWORDS pHAGE-PIK3CA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11696) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 11696) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11696 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..22 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 37..67 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 75..91 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 80..102 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 99..115 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 99..115 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 165..184 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" LTR 376..1009 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 1056..1181 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1678..1911 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2096..2140 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2433..2550 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2598..3776 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2829..3767 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3724..3744 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3796..3820 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(7031..7055) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 7063..7081 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(7106..7125) /label=T7 /note="T7 promoter, forward primer" promoter complement(7107..7125) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(7131..7151) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 7205..7246 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 7267..7834 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 7284..7836 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(7428..7445) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 7655..7674 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 7848..8447 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(7848..7867) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 8344..8364 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 8461..9049 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(8514..8534) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(8932..8943) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 9124..9357 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(9476..9494) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 9562..9666 /gene="bla" /label=AmpR promoter CDS 9667..10527 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(9885..9904) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 10698..11286 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 11187..11206 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 11440..11457 /label=L4440 /note="L4440 vector, forward primer" primer_bind 11549..11568 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" ORIGIN 1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg 61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat 121 tacgaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 181 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc 241 caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta 301 aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac 361 tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc 421 tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag 481 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata 541 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg 601 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc 661 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct 721 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt 781 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg 841 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 901 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 961 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 1021 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 1081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 1141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 1201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 1261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 1321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 1381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 1441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 1501 agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag 1561 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt 1621 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg 1681 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac 1741 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct 1801 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct 1861 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg 1921 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa 1981 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa 2041 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa 2101 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac 2161 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag 2221 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc 2281 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga 2341 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg 2401 ccgaattcac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg 2461 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta 2521 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca 2581 gtttggacta gtcgtgaggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag 2641 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg 2701 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag 2761 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca 2821 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc 2881 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc 2941 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg 3001 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc 3061 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg 3121 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta 3181 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg 3241 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc 3301 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc 3361 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag 3421 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa 3481 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc 3541 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg 3601 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag 3661 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca 3721 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaagcg 3781 gccctgcaga tatcaacaag tttgtacaaa aaagcaggca ccatgcctcc aagaccatca 3841 tcaggtgaac tgtggggcat ccacttgatg cccccaagaa tcctagtaga atgtttacta 3901 ccaaatggaa tgatagtgac tttagaatgc ctccgtgagg ctacattaat aaccataaag 3961 catgaactat ttaaagaagc aagaaaatac cccctccatc aacttcttca agatgaatct 4021 tcttacattt tcgtaagtgt tactcaagaa gcagaaaggg aagaattttt tgatgaaaca 4081 agacgacttt gtgaccttcg gctttttcaa ccctttttaa aagtaattga accagtaggc 4141 aaccgtgaag aaaagatcct caatcgagaa attggttttg ctatcggcat gccagtgtgt 4201 gaatttgata tggttaaaga tccagaagta caggacttcc gaagaaatat tctgaacgtt 4261 tgtaaagaag ctgtggatct tagggacctc aattcacctc atagtagagc aatgtatgtc 4321 tatcctccaa atgtagaatc ttcaccagaa ttgccaaagc acatatataa taaattagat 4381 aaagggcaaa taatagtggt gatctgggta atagtttctc caaataatga caagcagaag 4441 tatactctga aaatcaacca tgactgtgta ccagaacaag taattgctga agcaatcagg 4501 aaaaaaactc gaagtatgtt gctatcctct gaacaactaa aactctgtgt tttagaatat 4561 cagggcaagt atattttaaa agtgtgtgga tgtgatgaat acttcctaga aaaatatcct 4621 ctgagtcagt ataagtatat aagaagctgt ataatgcttg ggaggatgcc caatttgatg 4681 ttgatggcta aagaaagcct ttattctcaa ctgccaatgg actgttttac aatgccatct 4741 tattccagac gcatttccac agctacacca tatatgaatg gagaaacatc tacaaaatcc 4801 ctttgggtta taaatagtgc actcagaata aaaattcttt gtgcaaccta cgtgaatgta 4861 aatattcgag acattgataa gatctatgtt cgaacaggta tctaccatgg aggagaaccc 4921 ttatgtgaca atgtgaacac tcaaagagta ccttgttcca atcccaggtg gaatgaatgg 4981 ctgaattatg atatatacat tcctgatctt cctcgtgctg ctcgactttg cctttccatt 5041 tgctctgtta aaggccgaaa gggtgctaaa gaggaacact gtccattggc atggggaaat 5101 ataaacttgt ttgattacac agacactcta gtatctggaa aaatggcttt gaatctttgg 5161 ccagtacctc atggattaga agatttgctg aaccctattg gtgttactgg atcaaatcca 5221 aataaagaaa ctccatgctt agagttggag tttgactggt tcagcagtgt ggtaaagttc 5281 ccagatatgt cagtgattga agagcatgcc aattggtctg tatcccgaga agcaggattt 5341 agctattccc acgcaggact gagtaacaga ctagctagag acaatgaatt aagggaaaat 5401 gacaaagaac agctcaaagc aatttctaca cgagatcctc tctctgaaat cactgagcag 5461 gagaaagatt ttctatggag tcacagacac tattgtgtaa ctatccccga aattctaccc 5521 aaattgcttc tgtctgttaa atggaattct agagatgaag tagcccagat gtattgcttg 5581 gtaaaagatt ggcctccaat caaacctgaa caggctatgg aacttctgga ctgtaattac 5641 ccagatccta tggttcgagg ttttgctgtt cggtgcttgg aaaaatattt aacagatgac 5701 aaactttctc agtatttaat tcagctagta caggtcctaa aatatgaaca atatttggat 5761 aacttgcttg tgagattttt actgaagaaa gcattgacta atcaaaggat tgggcacttt 5821 ttcttttggc atttaaaatc tgagatgcac aataaaacag ttagccagag gtttggcctg 5881 cttttggagt cctattgtcg tgcatgtggg atgtatttga agcacctgaa taggcaagtc 5941 gaggcaatgg aaaagctcat taacttaact gacattctca aacaggagaa gaaggatgaa 6001 acacaaaagg tacagatgaa gtttttagtt gagcaaatga ggcgaccaga tttcatggat 6061 gctctacagg gctttctgtc tcctctaaac cctgctcatc aactaggaaa cctcaggctt 6121 gaagagtgtc gaattatgtc ctctgcaaaa aggccactgt ggttgaattg ggagaaccca 6181 gacatcatgt cagagttact gtttcagaac aatgagatca tctttaaaaa tggggatgat 6241 ttacggcaag atatgctaac acttcaaatt attcgtatta tggaaaatat ctggcaaaat 6301 caaggtcttg atcttcgaat gttaccttat ggttgtctgt caatcggtga ctgtgtggga 6361 cttattgagg tggtgcgaaa ttctcacact attatgcaaa ttcagtgcaa aggcggcttg 6421 aaaggtgcac tgcagttcaa cagccacaca ctacatcagt ggctcaaaga caagaacaaa 6481 ggagaaatat atgatgcagc cattgacctg tttacacgtt catgtgctgg atactgtgta 6541 gctaccttca ttttgggaat tggagatcgt cacaatagta acatcatggt gaaagacgat 6601 ggacaactgt ttcatataga ttttggacac tttttggatc acaagaagaa aaaatttggt 6661 tataaacgag aacgtgtgcc atttgttttg acacaggatt tcttaatagt gattagtaaa 6721 ggagcccaag aatgcacaaa gacaagagaa tttgagaggt ttcaggagat gtgttacaag 6781 gcttatctag ctattcgaca gcatgccaat ctcttcataa atcttttctc aatgatgctt 6841 ggctctggaa tgccagaact acaatctttt gatgacattg catacattcg aaagacccta 6901 gccttagata aaactgagca agaggctttg gagtatttca tgaaacaaat gaatgatgca 6961 catcatggtg gctggacaac aaaaatggat tggatcttcc acacaattaa acagcatgca 7021 ttgaactaga acccagcttt cttgtacaaa gtggtgatat ccaattaacc ctcactaaag 7081 gagtagaaag ttgaaattga ttatgcccta tagtgagtcg tattaccacc caacttttct 7141 atacaaagtg gttgatatcc agcacagtgg cggccgctcg agtctagagg gcccgcggtt 7201 cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta ccggttagta 7261 atgagatccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg 7321 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg 7381 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg 7441 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca 7501 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct 7561 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca 7621 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa 7681 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg 7741 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg 7801 ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacacatatg accgagtaca 7861 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg 7921 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc 7981 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt 8041 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg 8101 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg 8161 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc 8221 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc 8281 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc 8341 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc 8401 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctaaatc gatagatcct 8461 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct 8521 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt 8581 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg 8641 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact 8701 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct 8761 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg 8821 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc 8881 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc 8941 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt 9001 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgagatcctt 9061 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg 9121 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt 9181 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc 9241 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg 9301 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta 9361 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga 9421 gtgagaggcc cgggttaatt aaggaaaggg ctagatcatt cttgaagacg aaagggcctc 9481 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt 9541 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca 9601 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg 9661 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc 9721 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg 9781 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt 9841 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta 9901 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat 9961 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga 10021 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca 10081 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact 10141 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc 10201 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact 10261 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt 10321 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt 10381 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt 10441 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata 10501 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag 10561 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat 10621 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa 10681 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 10741 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt 10801 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg 10861 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc 10921 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga 10981 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc 11041 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc 11101 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 11161 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 11221 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 11281 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct 11341 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag 11401 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa 11461 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc 11521 agcaagctca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 11581 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc 11641 tccccgtggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaat //