LOCUS Exported 9079 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral expression of KRAS G12D. ACCESSION . VERSION . KEYWORDS pHAGE-KRAS-G12D SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9079) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 9079) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9079 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 3448..4014 /codon_start=1 /product="human oncoprotein generated by the G12V mutation in the small GTPase K-Ras" /label=K-Ras (G12V) /translation="MTEYKLVVVGADGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI RKHKEKMSKDGKKKKKKSKTKCVIM" CDS 3448..4014 /codon_start=1 /product="human small GTPase proto-oncoprotein" /label=K-Ras /translation="MTEYKLVVVGADGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI RKHKEKMSKDGKKKKKKSKTKCVIM" protein_bind complement(4016..4040) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 4093..4134 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 4155..4722 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 4172..4724 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(4316..4333) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 4543..4562 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 4736..5455 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 4736..5452 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(4781..4802) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(5042..5061) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 5389..5410 /label=EGFP-C /note="EGFP, forward primer" misc_feature 5469..6057 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5522..5542) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(5940..5951) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 6132..6365 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(6484..6502) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 6570..6674 /gene="bla" /label=AmpR promoter CDS 6675..7535 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(6893..6912) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 7706..8294 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8195..8214 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 8448..8465 /label=L4440 /note="L4440 vector, forward primer" primer_bind 8557..8576 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 8705..8726 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 8741..8771 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8779..8795 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 8784..8806 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 8803..8819 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 8803..8819 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 8869..8888 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagc aggcaccatg actgaatata aacttgtggt agttggagct 3481 gatggcgtag gcaagagtgc cttgacgata cagctaattc agaatcattt tgtggacgaa 3541 tatgatccaa caatagagga ttcctacagg aagcaagtag taattgatgg agaaacctgt 3601 ctcttggata ttctcgacac agcaggtcaa gaggagtaca gtgcaatgag ggaccagtac 3661 atgaggactg gggagggctt tctttgtgta tttgccataa ataatactaa atcatttgaa 3721 gatattcacc attatagaga acaaattaaa agagttaagg actctgaaga tgtacctatg 3781 gtcctagtag gaaataaatg tgatttgcct tctagaacag tagacacaaa acaggctcag 3841 gacttagcaa gaagttatgg aattcctttt attgaaacat cagcaaagac aagacagggt 3901 gttgatgatg ccttctatac attagttcga gaaattcgaa aacataaaga aaagatgagc 3961 aaagatggta aaaagaagaa aaagaagtca aagacaaagt gtgtaattat gtagaaccca 4021 gctttcttgt acaaagtggt tgatatccag cacagtggcg gccgctcgag tctagagggc 4081 ccgcggttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc 4141 ggttagtaat gagatccctc ccccccccct aacgttactg gccgaagccg cttggaataa 4201 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg 4261 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc 4321 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct 4381 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac 4441 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc 4501 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta 4561 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg 4621 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg 4681 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca cacatatggt 4741 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga 4801 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa 4861 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt 4921 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca 4981 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa 5041 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa 5101 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct 5161 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat 5221 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca 5281 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct 5341 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct 5401 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaaatcga 5461 tagatcctaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact 5521 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg 5581 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg 5641 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa 5701 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc 5761 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg 5821 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt 5881 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt 5941 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc 6001 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg 6061 agatccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga 6121 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg 6181 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa 6241 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct 6301 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc 6361 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat 6421 atcagagagt gagaggcccg ggttaattaa ggaaagggct agatcattct tgaagacgaa 6481 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga 6541 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa 6601 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt 6661 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg 6721 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag 6781 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg 6841 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg 6901 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt 6961 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga 7021 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac 7081 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc 7141 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc 7201 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac 7261 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag 7321 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg 7381 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta 7441 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg 7501 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata 7561 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt 7621 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 7681 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 7741 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 7801 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag 7861 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 7921 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 7981 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 8041 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 8101 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 8161 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 8221 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 8281 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 8341 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 8401 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga 8461 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 8521 attaatgcag caagctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 8581 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 8641 caaaaagctc cccgtggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg 8701 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg 8761 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca 8821 tgattacgaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 8881 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc 8941 atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac 9001 tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat 9061 gtactacaaa cttagtagt //