LOCUS       Exported                8173 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Doxycycline-inducible expression of p16-INK4A product of CDKN2A.
ACCESSION   .
VERSION     .
KEYWORDS    pLX401-INK4A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8173)
  AUTHORS   Howard TP, Arnoff TE, Song MR, Giacomelli AO, Wang X, Hong AL, 
            Dharia NV, Wang S, Vazquez F, Pham MT, Morgan AM, Wachter F, Bird 
            GH, Kugener G, Oberlick EM, Rees MG, Tiv HL, Hwang JH, Walsh KH, 
            Cook A, Krill-Burger JM, Tsherniak A, Gokhale PC, Park PJ, Stegmaier
            K, Walensky LD, Hahn WC, Roberts CWM
  TITLE     MDM2 and MDM4 are Therapeutic Vulnerabilities in Malignant Rhabdoid 
            Tumors.
  JOURNAL   Cancer Res. 2019 Feb 12. pii: 0008-5472.CAN-18-3066. doi: 
            10.1158/0008-5472.CAN-18-3066.
  PUBMED    30755442
REFERENCE   2  (bases 1 to 8173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8173
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     98..115
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    232..253
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        268..298
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    306..322
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     311..333
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     330..346
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     330..346
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     365..385
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        367..385
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        413..639
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             640..820
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    867..992
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1485..1718
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1903..1947
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2160..2277
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2331..2645
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2339..2357
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2375..2393
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2410..2428
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2446..2464
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2482..2500
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2517..2535
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2553..2571
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2618..2642
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2666..2690
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3165..3189)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3207..3224
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        3234..3744
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3663..3681
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3754..4350
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3754..3773)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4250..4270
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4351..4404
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4405..5151
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5191..5211)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5609..5620)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5825..6058
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6130..6251
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6167..6186)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6221..6240
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6291..6426
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6353..6372
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6426..6497
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6498..7358
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6716..6735)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7529..8117
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8018..8037
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
       61 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
      121 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      181 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      241 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      301 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
      361 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct
      421 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
      481 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
      541 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
      601 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
      661 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
      721 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
      781 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
      841 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
      901 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
      961 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     1021 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     1081 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     1141 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     1201 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     1261 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     1321 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     1381 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     1441 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     1501 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     1561 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     1621 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     1681 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     1741 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     1801 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     1861 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     1921 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     1981 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     2041 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     2101 ctcccaaccc cgaggggaca attctcgacc tcgagacaaa tggcagtatt catccacaat
     2161 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     2221 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
     2281 gtttattaca gggacagcag agatccactt tggccgcgaa tcgatatgtc gagtttactc
     2341 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgatgt
     2401 cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata
     2461 gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttatccc
     2521 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
     2581 gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg aaccgtcaga
     2641 tcgcctggag aattggctag catcaacaag tttgtacaaa aaagcaggct tcaccatgga
     2701 acccgctgct gggtccagca tggagccgtc agccgattgg ctggctaccg ctgcggctcg
     2761 aggacgcgta gaagaagttc gagcgctttt ggaagcggga gctcttccta acgcgccaaa
     2821 ttcatacggt aggaggccga tccaagttat gatgatgggc agcgctcggg tcgcagaatt
     2881 gctgctgctc cacggcgctg agcccaactg tgcagatcct gcaaccctca cgcggccagt
     2941 acatgacgct gccagggaag gtttcctgga tacgttggta gtattgcatc gcgctggcgc
     3001 gcggcttgat gttcgagatg catggggtag gctcccggtg gacctggcgg aagagctggg
     3061 acacagagac gttgcgagat atctccgagc tgctgcagga gggacacgcg ggtcaaacca
     3121 cgccaggatc gatgccgccg aaggtccatc agatattcct gatcacccag ctttcttgta
     3181 caaagtggtt tagtaatgaa ccggtccacc accaccacca ccactaagga tccggggttg
     3241 gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt
     3301 ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc
     3361 agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg
     3421 cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc
     3481 gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga
     3541 ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg
     3601 gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg
     3661 cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac
     3721 cgaatcaccg acctctctcc ccagcaattc accatgaccg agtacaagcc cacggtgcgc
     3781 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     3841 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     3901 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     3961 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     4021 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     4081 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     4141 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     4201 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     4261 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     4321 acctggtgca tgacccgcaa gcccggtgcc gaaggtagag gttctctcct cacttgtggt
     4381 gatgttgaag aaaaccctgg tccaatgtct agactggaca agagcaaagt cataaacgga
     4441 gctctggaat tactcaatgg tgtcggtatc gaaggcctga cgacaaggaa actcgctcaa
     4501 aagctgggag ttgagcagcc taccctgtac tggcacgtga agaacaagcg ggccctgctc
     4561 gatgccctgc caatcgagat gctggacagg catcataccc acttctgccc cctggaaggc
     4621 gagtcatggc aagactttct gcggaacaac gccaagtcat accgctgtgc tctcctctca
     4681 catcgcgacg gggctaaagt gcatctcggc acccgcccaa cagagaaaca gtacgaaacc
     4741 ctggaaaatc agctcgcgtt cctgtgtcag caaggcttct ccctggagaa cgcactgtac
     4801 gctctgtccg ccgtgggcca ctttacactg ggctgcgtat tggaggaaca ggagcatcaa
     4861 gtagcaaaag aggaaagaga gacacctacc accgattcta tgcccccact tctgagacaa
     4921 gcaattgagc tgttcgaccg gcagggagcc gaacctgcct tccttttcgg cctggaacta
     4981 atcatatgtg gcctggagaa acagctaaag tgcgaaagcg gcgggccgac cgacgccctt
     5041 gacgattttg acttagacat gctcccagcc gatgcccttg acgactttga ccttgatatg
     5101 ctgcctgctg acgctcttga cgattttgac cttgacatgc tccccgggta actaagtaag
     5161 gatcgatcca agatatcgta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     5221 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     5281 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     5341 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     5401 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     5461 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     5521 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     5581 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     5641 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     5701 gatctccctt tgggccgcct ccccgcctgt ttcgcctcgg cgtccggact agaggtacct
     5761 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     5821 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     5881 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     5941 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     6001 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     6061 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     6121 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     6181 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     6241 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     6301 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     6361 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     6421 gaggcctttc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     6481 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     6541 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     6601 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     6661 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     6721 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     6781 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     6841 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     6901 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     6961 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     7021 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     7081 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     7141 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     7201 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     7261 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     7321 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     7381 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     7441 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     7501 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     7561 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     7621 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     7681 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     7741 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     7801 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     7861 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     7921 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     7981 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     8041 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     8101 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     8161 ggccttttgc tca
//
