LOCUS       Exported                2181 bp ds-DNA     linear   SYN 22-MAY-2021
DEFINITION  Gene knockout/in for actinomycetes.
ACCESSION   .
VERSION     .
KEYWORDS    pCRISPR-Cas9-ScaligD
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Tong Y, Charusanti P, Zhang L, Weber T, Lee SY
  TITLE     CRISPR-Cas9 Based Engineering of Actinomycetal Genomes.
  JOURNAL   ACS Synth Biol. 2015 Sep 18;4(9):1020-9. doi: 
            10.1021/acssynbio.5b00038. Epub 2015 Apr 7.
  PUBMED    25806970
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 22, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
ORIGIN
        1 atcgaggtcc ggctgagcaa cctggacaag gtgctctatc cagcgaccgg caccaccaag
       61 ggcgaggtca tcgagtacta cgccgaaatc gccccggcga tgctgccgca tatcgcgggc
      121 cggccgatca cccggaaacg gtggccgaac ggtgtcgccg aatcgtcgtt cttcgagaag
      181 aacctcggcg cgggtacacc gtcgtggcta ccgcgccgtg cccaggaaca ttccgaccgc
      241 accgcgcact atccggtgat ctcgtcgcag gccggcctgg tctggctggg tcagcaggcc
      301 gccctggaga tccacgtacc gcaatggcgc ttcgacggcg atgcgcgcgg acccgcgacg
      361 cggctggtgt tcgatctcga tcccggcccc ggcgcgggac tgcccgaatg cgcgcgggtg
      421 gcgctcgggg tgcgggatat ggtcgccgaa atcgggatgc gcgcgttccc gctgaccagc
      481 ggtagcaaag gtatccacct gtacgtcccg ctggaccggg tgctgagccc cggcggggcg
      541 tccacggtgg ccaaacaggt cgccgcgaat ctggagaaac tccttcccga cctggtcacc
      601 gccacgatgg cgaagagtgt gcgggccggg aaggtgttcc tggactggag tcagaacaac
      661 ccgtccaaga cgaccatcgc accgtattcg ctgcgcggcc gcgagcagcc gaacgtcgcc
      721 gcaccacgcc actgggcgga gctcgaggac gcccgtgaac tgcggcagct gcggttcgac
      781 gaagttctgg agcgttatcg gtccgagggt gatctgctgg ccggcctgga tacacccctg
      841 aacgacgcgt tgacgaaata ccgatcgatg cgtgacccgg cgcgtacacc ggagccggta
      901 ccgccgcatt cgccccggcc cggccccggt gaccgctatg tcgtccacga acaccacgcc
      961 cggcggttgc actgggatgt gcggttggaa cgcgacgggg tgctggtgtc gtgggcggtg
     1021 cccaaggggc cgccggaaag cacccggcag aatcggctcg ccgtgcacac cgaggaccac
     1081 ccgctggaat acctggactt ccacggcacg atcccggccg gcgagtacgg ggcaggggag
     1141 ctgtcggtct gggataccgg cacctaccgc gccgagaaat ggcgcgacga cgaggtgatc
     1201 gtggttttcc ggggcgagcg gctcaacggc cggtacgcca tgatccggac cgagggcgat
     1261 caatggctga tgcatctcat gaaggaccag cccgcgaccg gggaactgcc gcgtggactc
     1321 acccccatgc tggccaccag tggcgaagtg gccgggctgc cggactcgga gtgggcgttc
     1381 gaacgtaaat gggacggata ccggctgctc gtcgaaatcg atgccggcga aatgcggctg
     1441 cgcagccggg ccggtaacga cgtcaccgcg cgctatcccc agttgtcggt gctggccgag
     1501 gagctggccg accatcaggt gatactcgac ggtgagctca tcgtccgcgg ccccgacggc
     1561 gcggtgaata tcgcgctgtt gaaggcgaat ccgcggcgcg ccgaattcct ggcgttcgat
     1621 ctgctgttcc tcgacggcac ttcactgctg cgcaaacgct accgcgatcg gcggcacgtg
     1681 ctcgaagcgc tggccgcgac caccaccgaa ctccgggtgc caccgcgcta tgagggcgac
     1741 ggcaccgagg ccctgcaccg cagcgaagaa gatggcgccg agggcgtgat cgccaaacgg
     1801 ctggattcgg tgtatctgcc cgggacccgc gggcattcgt gggtgaagca ccggaactgg
     1861 cgtacccagg aggtggtgat cgggggtatg cggcgcagta aggcgcgacc gttcgcctcg
     1921 ttgctggtcg ggataccggc cgaggacggc ctggtgtatg cgggccgggt cgggaccggg
     1981 ttcgacgaag cggggatgac cgaactcgcg gcccggctgc gccggtcgga acgtaagacg
     2041 ccgccgttca ccaacgagat gtcggccgat gaactccggg acgcgatctg ggtgacaccg
     2101 aagatcaaag gcactgttcg ctacatggat tggaccgacg gcggacgctt ctggcatcct
     2161 gcctggctcg gcgaggtgtg a
//
