LOCUS Exported 1824 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION LexA UAS. ACCESSION . VERSION . KEYWORDS pWS482 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1824) AUTHORS Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N, Wigglesworth M, Ladds G, Ellis T TITLE Engineering a Model Cell for Rational Tuning of GPCR Signaling. JOURNAL Cell. 2019 Apr 18;177(3):782-796.e27. doi: 10.1016/j.cell.2019.02.023. Epub 2019 Apr 4. PUBMED 30955892 REFERENCE 2 (bases 1 to 1824) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1824 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 191..225 /label=lambda t0 terminator /note="minimal transcription terminator from phage lambda (Scholtissek and Grosse, 1987)" CDS complement(296..955) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 870..889 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" rep_origin 1137..1725 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1626..1645 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 agtcggtctc aaacgggtag tccatcgttg taggatactg tatataaata cagtagtggt 61 catcgatact gtatataaaa ccagtaggca atcgtttgta ctgtatgtac atacagtata 121 cctcgcaact actgtatata aacacagtaa ttaacttgta atattctaat caattgatga 181 gaccagacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag 241 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg 301 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga 361 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt 421 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt 481 ttaaatcaaa actggtgaaa ctcacccagg gattggctga aacgaaaaac atattctcaa 541 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata 601 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag 661 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt 721 ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa 781 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca 841 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt 901 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag 961 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt 1021 cattatggtg aaagttggaa cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa 1081 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 1141 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 1201 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 1261 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag 1321 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 1381 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 1441 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 1501 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 1561 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 1621 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 1681 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 1741 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 1801 tcccctgatt ctgtggataa ccgt //