LOCUS       Exported                1793 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  LexA UAS.
ACCESSION   .
VERSION     .
KEYWORDS    pWS483
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N,
            Wigglesworth M, Ladds G, Ellis T
  TITLE     Engineering a Model Cell for Rational Tuning of GPCR Signaling.
  JOURNAL   Cell. 2019 Apr 18;177(3):782-796.e27. doi: 
            10.1016/j.cell.2019.02.023. Epub 2019 Apr 4.
  PUBMED    30955892
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      160..194
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(265..924)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     839..858
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1106..1694
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1595..1614
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc aaacgggtag tccatcgttg taggatactg tatataaaac cagtaggcaa
       61 tcgtttgtac tgtatgtaca tacagtatac ctcgcaacta ctgtatataa acacagtaat
      121 taacttgtaa tattctaatc aattgatgag accagaccaa taaaaaacgc ccggcggcaa
      181 ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg
      241 agtccaagcg agctcgatat caaattacgc cccgccctgc cactcatcgc agtactgttg
      301 taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat
      361 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
      421 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
      481 attggctgaa acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
      541 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
      601 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
      661 aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc
      721 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt
      781 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc
      841 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt
      901 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc
      961 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg
     1021 cccgatcaat catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     1081 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     1141 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     1201 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     1261 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     1321 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     1381 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     1441 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     1501 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     1561 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     1621 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     1681 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     1741 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgt
//
