LOCUS Exported 1730 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Core promoter. ACCESSION . VERSION . KEYWORDS pWS485 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1730) AUTHORS Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N, Wigglesworth M, Ladds G, Ellis T TITLE Engineering a Model Cell for Rational Tuning of GPCR Signaling. JOURNAL Cell. 2019 Apr 18;177(3):782-796.e27. doi: 10.1016/j.cell.2019.02.023. Epub 2019 Apr 4. PUBMED 30955892 REFERENCE 2 (bases 1 to 1730) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1730 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 97..131 /label=lambda t0 terminator /note="minimal transcription terminator from phage lambda (Scholtissek and Grosse, 1987)" CDS complement(202..861) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 776..795 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" rep_origin 1043..1631 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1532..1551 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 agtcggtctc aaacgggtag tccatcgttg taggatactg tatataaaca cagtaattaa 61 cttgtaatat tctaatcaat tgatgagacc agaccaataa aaaacgcccg gcggcaaccg 121 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt 181 ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcatcgcagt actgttgtaa 241 ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc 301 cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc 361 gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt 421 ggctgaaacg aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc 481 gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc 541 actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac 601 actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcatt 661 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac 721 ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac 781 tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata 841 tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa 901 aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccc 961 gatcaatcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 1021 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 1081 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 1141 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt 1201 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 1261 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 1321 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 1381 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 1441 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 1501 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 1561 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 1621 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 1681 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt //