LOCUS       Exported                1812 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Core promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pWS487
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N,
            Wigglesworth M, Ladds G, Ellis T
  TITLE     Engineering a Model Cell for Rational Tuning of GPCR Signaling.
  JOURNAL   Cell. 2019 Apr 18;177(3):782-796.e27. doi: 
            10.1016/j.cell.2019.02.023. Epub 2019 Apr 4.
  PUBMED    30955892
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     26..49
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     terminator      179..213
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(284..943)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     858..877
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1125..1713
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1614..1633
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc attgatatat aaagacggta ggtattgatt gtaattctgt aaatctattt
       61 cttaaacttc ttaaattcta cttttatagt tagtcttttt tttagtttta aaacaccaag
      121 aacttagttt cgaataaaca cacataaaca aacaaaagat ctatgtgaga ccagaccaat
      181 aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat
      241 tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc
      301 actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa
      361 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt
      421 tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac
      481 tggtgaaact cacccaggga ttggctgaaa cgaaaaacat attctcaata aaccctttag
      541 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact
      601 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga
      661 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca
      721 tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa
      781 acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct
      841 ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt
      901 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc
      961 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa
     1021 agttggaacc tcttacgtgc ccgatcaatc atgaccaaaa tcccttaacg tgagttttcg
     1081 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     1141 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     1201 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     1261 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     1321 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     1381 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     1441 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     1501 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     1561 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     1621 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     1681 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     1741 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     1801 gtggataacc gt
//
