LOCUS Exported 1801 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Core promoter. ACCESSION . VERSION . KEYWORDS pWS493 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1801) AUTHORS Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N, Wigglesworth M, Ladds G, Ellis T TITLE Engineering a Model Cell for Rational Tuning of GPCR Signaling. JOURNAL Cell. 2019 Apr 18;177(3):782-796.e27. doi: 10.1016/j.cell.2019.02.023. Epub 2019 Apr 4. PUBMED 30955892 REFERENCE 2 (bases 1 to 1801) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1801 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 168..202 /label=lambda t0 terminator /note="minimal transcription terminator from phage lambda (Scholtissek and Grosse, 1987)" CDS complement(273..932) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 847..866 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" rep_origin 1114..1702 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1603..1622 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 agtcggtctc attgatatat aaatgtaata agaagtttgg taatattcaa ttcgaagtgt 61 tcagtctttt acttctcttg ttttatagaa gaaaaaacat caagaaacat ctttaacata 121 cacaaacaca tactatcaga atacaagatc tatgtgagac cagaccaata aaaaacgccc 181 ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta 241 tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag 301 tactgttgta attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga 361 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg 421 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc 481 acccagggat tggctgaaac gaaaaacata ttctcaataa accctttagg gaaataggcc 541 aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg 601 tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa 661 caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc 721 ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta 781 tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta 841 cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca 901 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc 961 gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct 1021 cttacgtgcc cgatcaatca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 1081 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 1141 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 1201 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 1261 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 1321 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 1381 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 1441 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 1501 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 1561 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 1621 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 1681 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 1741 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg 1801 t //