LOCUS Exported 1796 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Core promoter. ACCESSION . VERSION . KEYWORDS pRC246 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1796) AUTHORS Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N, Wigglesworth M, Ladds G, Ellis T TITLE Engineering a Model Cell for Rational Tuning of GPCR Signaling. JOURNAL Cell. 2019 Apr 18;177(3):782-796.e27. doi: 10.1016/j.cell.2019.02.023. Epub 2019 Apr 4. PUBMED 30955892 REFERENCE 2 (bases 1 to 1796) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1796 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 163..197 /label=lambda t0 terminator /note="minimal transcription terminator from phage lambda (Scholtissek and Grosse, 1987)" CDS complement(268..927) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 842..861 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" rep_origin 1109..1697 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1598..1617 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 agtcggtctc attgacaata ttatttaagg acctattgtt ttttccaata ggtggttagc 61 aatcgtctta ctttctaact tttcttacct tttacatttc agcaatatat atatatatat 121 ttcaaggata taccattcta agatctatgt gagaccagac caataaaaaa cgcccggcgg 181 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac 241 aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcat cgcagtactg 301 ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg 361 aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac 421 gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca 481 gggattggct gaaacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt 541 ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg 601 gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg 661 gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa attccggatg 721 agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt 781 ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg 841 agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt 901 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa 961 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac 1021 gtgcccgatc aatcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 1081 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 1141 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 1201 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc 1261 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 1321 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 1381 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 1441 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 1501 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 1561 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 1621 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 1681 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 1741 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgt //