LOCUS Exported 1772 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Core promoter. ACCESSION . VERSION . KEYWORDS pWS498 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1772) AUTHORS Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N, Wigglesworth M, Ladds G, Ellis T TITLE Engineering a Model Cell for Rational Tuning of GPCR Signaling. JOURNAL Cell. 2019 Apr 18;177(3):782-796.e27. doi: 10.1016/j.cell.2019.02.023. Epub 2019 Apr 4. PUBMED 30955892 REFERENCE 2 (bases 1 to 1772) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1772 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 139..173 /label=lambda t0 terminator /note="minimal transcription terminator from phage lambda (Scholtissek and Grosse, 1987)" CDS complement(244..903) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 818..837 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" rep_origin 1085..1673 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1574..1593 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 agtcggtctc attgatatat aagcgctgat gttttgctaa gtcgaggtta gtatggcttc 61 atctctcatg agaataagaa caacaacaaa tagagcaagc aaattcgaga ttaccaagat 121 ctatgtgaga ccagaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc 181 agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc 241 aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc 301 gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt 361 gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt 421 ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaaa cgaaaaacat 481 attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg 541 cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa 601 cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag 661 ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat 721 gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt 781 aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa 841 atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc 901 cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga 961 tcttatttca ttatggtgaa agttggaacc tcttacgtgc ccgatcaatc atgaccaaaa 1021 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat 1081 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 1141 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 1201 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc 1261 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg 1321 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg 1381 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 1441 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg 1501 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga 1561 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 1621 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 1681 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc 1741 ctgcgttatc ccctgattct gtggataacc gt //