LOCUS Exported 1794 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Core promoter. ACCESSION . VERSION . KEYWORDS pWS496 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1794) AUTHORS Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N, Wigglesworth M, Ladds G, Ellis T TITLE Engineering a Model Cell for Rational Tuning of GPCR Signaling. JOURNAL Cell. 2019 Apr 18;177(3):782-796.e27. doi: 10.1016/j.cell.2019.02.023. Epub 2019 Apr 4. PUBMED 30955892 REFERENCE 2 (bases 1 to 1794) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1794 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 161..195 /label=lambda t0 terminator /note="minimal transcription terminator from phage lambda (Scholtissek and Grosse, 1987)" CDS complement(266..925) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 840..859 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" rep_origin 1107..1695 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1596..1615 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 agtcggtctc attgatatat aaaactcttg ttttcttctt ttctctaaat attctttcct 61 tatacattag gacctttgca gcataaatta ctatacttct atagacacac aaacacaaat 121 acacacacta aattaataag atctatgtga gaccagacca ataaaaaacg cccggcggca 181 accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag 241 gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt 301 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa 361 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg 421 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg 481 gattggctga aacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt 541 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt 601 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt 661 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag 721 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct 781 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag 841 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg 901 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact 961 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt 1021 gcccgatcaa tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 1081 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 1141 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 1201 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 1261 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 1321 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 1381 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 1441 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 1501 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 1561 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 1621 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 1681 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 1741 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgt //