LOCUS Exported 2661 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Adenosine GPCR. ACCESSION . VERSION . KEYWORDS pWS625 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2661) AUTHORS Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N, Wigglesworth M, Ladds G, Ellis T TITLE Engineering a Model Cell for Rational Tuning of GPCR Signaling. JOURNAL Cell. 2019 Apr 18;177(3):782-796.e27. doi: 10.1016/j.cell.2019.02.023. Epub 2019 Apr 4. PUBMED 30955892 REFERENCE 2 (bases 1 to 2661) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2661 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 1028..1062 /label=lambda t0 terminator /note="minimal transcription terminator from phage lambda (Scholtissek and Grosse, 1987)" CDS complement(1133..1792) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 1707..1726 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" rep_origin 1974..2562 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2463..2482 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 agtcggtctc atatgttgtt ggaaacacaa gatgcgttgt atgttgcgtt ggaattggta 61 attgctgcgc tatcggttgc gggaaatgtt ttggtttgtg ctgcggttgg aacggcgaat 121 accttgcaaa cgcctactaa ttattttttg gtttcattgg cagcggctga tgttgctgtt 181 ggactatttg ctattccttt cgctattact atttctttgg gattttgtac cgatttttat 241 ggatgtctat ttctagcttg ttttgttttg gttctaacgc aatcttcaat tttttctcta 301 ttggctgttg ccgtagatag gtatttggct atttgcgtac cgctaaggta caagtccctt 361 gtaacgggaa ctcgtgccag gggagtaata gcagtactat gggtactagc tttcggaatt 421 ggacttaccc cttttttggg atggaattcc aaggattccg ctactaataa ttgtacagag 481 ccttgggacg gaactacgaa cgagtcttgt tgtctagtta aatgcctatt cgaaaacgtt 541 gtacctatgt cttatatggt gtactttaac tttttcggat gcgtgttgcc tcctttgcta 601 atcatgttgg ttatttatat aaaaattttt ttggttgctt gtaggcaact acaacgtacc 661 gaattgatgg atcattcgag gactactcta caaagggaaa ttcacgccgc taaatccttg 721 gctatgatag ttggaatatt cgctttgtgt tggctccctg ttcacgcagt gaattgcgta 781 accctatttc aacctgcaca aggcaagaac aaacctaaat gggccatgaa catggctata 841 ctattgtccc acgctaactc cgtggtaaac cctatagtat acgcatatag gaatcgtgat 901 tttcgttata ccttccataa gataatttca aggtacctac tatgtcaggc cgacgtaaaa 961 tccggaaacg gccaagcagg agtgcaacct gcactaggcg ttggactcgg atcctgagac 1021 cagaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc 1081 tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc 1141 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc 1201 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg 1261 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta 1321 aatcaaaact ggtgaaactc acccagggat tggctgaaac gaaaaacata ttctcaataa 1381 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt 1441 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt 1501 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt 1561 tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg 1621 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct 1681 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac 1741 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt 1801 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat 1861 tatggtgaaa gttggaacct cttacgtgcc cgatcaatca tgaccaaaat cccttaacgt 1921 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 1981 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 2041 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 2101 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac 2161 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 2221 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 2281 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 2341 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 2401 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 2461 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 2521 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 2581 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 2641 cctgattctg tggataaccg t //