LOCUS Exported 2715 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Melatonin GPCR. ACCESSION . VERSION . KEYWORDS pWS1806 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2715) AUTHORS Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N, Wigglesworth M, Ladds G, Ellis T TITLE Engineering a Model Cell for Rational Tuning of GPCR Signaling. JOURNAL Cell. 2019 Apr 18;177(3):782-796.e27. doi: 10.1016/j.cell.2019.02.023. Epub 2019 Apr 4. PUBMED 30955892 REFERENCE 2 (bases 1 to 2715) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2715 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 1082..1116 /label=lambda t0 terminator /note="minimal transcription terminator from phage lambda (Scholtissek and Grosse, 1987)" CDS complement(1187..1846) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 1761..1780 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" rep_origin 2028..2616 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2517..2536 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 agtcggtctc atatgcaagg taatggttct gctttgccaa atgcttctca accagttttg 61 agaggtgatg gtgctagacc ttcttggttg gcttctgctt tagcttgtgt tttgattttc 121 accatcgttg tcgatatctt gggtaacttg ttggttatct tgtccgtcta ccgtaacaag 181 aaattgagaa acgctggtaa catcttcgtt gtttctttgg ctgttgctga tttggttgtt 241 gctatctatc catatccact ggtcttgatg tccattttta acaacggttg gaacttgggt 301 tacttgcatt gtcaagtttc tggtttcttg atgggtttgt ccgttattgg ttccattttc 361 aacattaccg gtatcgccat taacaggtac tgttacattt gccactcact gaagtacgac 421 aagttgtact cttctaagaa ctccttgtgc tacgttttgt tgatctggtt gttaactttg 481 gctgctgttt tgcctaattt gagagctggt acattgcaat acgatccaag aatctactct 541 tgtaccttcg ctcaatctgt ttcttctgct tacactattg ccgttgtcgt tttccatttt 601 ttggtcccaa tgattatcgt catcttctgc tacttgagaa tctggatttt ggtcttgcaa 661 gtcagacaaa gagttaagcc agatagaaag ccaaaattga agccacaaga cttcagaaac 721 ttcgttacca tgtttgtggt tttcgttttg ttcgctattt gttgggctcc attgaacttt 781 attggtttgg cagttgcttc tgatccagct tctatggttc caagaattcc agaatggttg 841 ttcgttgctt cttactacat ggcttacttc aactcttgtt tgaacgccat tatctacggc 901 ttgttgaatc agaactttag gaaagagtac aggcgtatca tcgtttcttt gtgtactgct 961 agagttttct tcgtcgattc ctctaatgat gttgccgata gagttaagtg gaaaccatct 1021 ccattgatga ccaacaacaa tgttgtcaag gttgactccg ttggatcctg agaccagacc 1081 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt 1141 cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct 1201 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac 1261 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat 1321 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa 1381 aactggtgaa actcacccag ggattggctg aaacgaaaaa catattctca ataaaccctt 1441 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa 1501 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat 1561 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg 1621 ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat 1681 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg 1741 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc 1801 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag 1861 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt 1921 gaaagttgga acctcttacg tgcccgatca atcatgacca aaatccctta acgtgagttt 1981 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 2041 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 2101 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 2161 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta 2221 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 2281 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 2341 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 2401 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 2461 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 2521 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 2581 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 2641 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 2701 tctgtggata accgt //