LOCUS Exported 2322 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION TetR bacterial repressor. ACCESSION . VERSION . KEYWORDS pWS931 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2322) AUTHORS Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N, Wigglesworth M, Ladds G, Ellis T TITLE Engineering a Model Cell for Rational Tuning of GPCR Signaling. JOURNAL Cell. 2019 Apr 18;177(3):782-796.e27. doi: 10.1016/j.cell.2019.02.023. Epub 2019 Apr 4. PUBMED 30955892 REFERENCE 2 (bases 1 to 2322) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2322 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(86..105) /label=Tet-R /note="Tetracycline resistance gene, reverse primer" CDS 643..663 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" terminator 689..723 /label=lambda t0 terminator /note="minimal transcription terminator from phage lambda (Scholtissek and Grosse, 1987)" CDS complement(794..1453) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 1368..1387 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" rep_origin 1635..2223 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2124..2143 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 agtcggtctc atatgtctag attagataaa agtaaagtga ttaacagcgc attagagctg 61 cttaatgagg tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta 121 gagcagccta cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc 181 attgagatgt tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa 241 gattttttac gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga 301 gcaaaagtac atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa 361 ttagcctttt tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct 421 gtggggcatt ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa 481 gaaagggaaa cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta 541 tttgatcacc aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga 601 ttagaaaaac aacttaaatg tgaaagtgga tcaggttctc ctccaaaaaa aaagagaaaa 661 gttggatctg gttcttgaga ccagaccaat aaaaaacgcc cggcggcaac cgagcgttct 721 gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga 781 gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa 841 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca 901 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt 961 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaaa 1021 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg 1081 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga 1141 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc 1201 atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc 1261 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta 1321 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa 1381 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga 1441 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc 1501 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc ccgatcaatc 1561 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 1621 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 1681 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 1741 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag 1801 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 1861 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 1921 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 1981 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 2041 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 2101 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 2161 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 2221 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 2281 atgttctttc ctgcgttatc ccctgattct gtggataacc gt //