LOCUS       Exported                2358 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Blue fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    pRC063
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Oling D, Larsson N,
            Wigglesworth M, Ladds G, Ellis T
  TITLE     Engineering a Model Cell for Rational Tuning of GPCR Signaling.
  JOURNAL   Cell. 2019 Apr 18;177(3):782-796.e27. doi: 
            10.1016/j.cell.2019.02.023. Epub 2019 Apr 4.
  PUBMED    30955892
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      725..759
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(830..1489)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1404..1423
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1671..2259
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2160..2179
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc atatgagtga gttgattaag gaaaacatgc acatgaagtt gtacatggaa
       61 ggaacagtag ataatcatca tttcaaatgt acgtcagaag gtgagggcaa accttatgaa
      121 ggaactcaaa ctatgagaat taaagtcgtt gagggtggcc cactaccatt tgcattcgat
      181 atcctcgcaa catcttttct gtatggttct aaaaccttta tcaaccatac acaaggcata
      241 ccagatttct ttaagcaatc atttcctgaa ggtttcacat gggaaagagt aactacctat
      301 gaagatggtg gtgtccttac agctactcaa gacacgtctt tgcaggatgg ttgtttaatc
      361 tacaatgtta agataagagg agtgaacttt acatccaatg gaccagtcat gcaaaagaaa
      421 actctaggtt gggaggcatt cacagaaact ttgtacccag ccgatggggg cttagaaggg
      481 agaaatgaca tggctttaaa actggttggc ggtagccatt tgattgcgaa cgctaaaaca
      541 acctacagaa gcaaaaagcc agctaaaaat cttaagatgc ctgtatacta cgtggattac
      601 agattagaaa ggaaggaagc taataatgaa acttacgttg aacagcacga ggtggccgtt
      661 gcccgttatt gcgaccttcc ttcaaaattg ggacacaaac taaacggatc ctgagaccag
      721 accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga
      781 ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc
      841 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
      901 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
      961 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
     1021 caaaactggt gaaactcacc cagggattgg ctgaaacgaa aaacatattc tcaataaacc
     1081 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
     1141 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
     1201 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
     1261 ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
     1321 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
     1381 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
     1441 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
     1501 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
     1561 ggtgaaagtt ggaacctctt acgtgcccga tcaatcatga ccaaaatccc ttaacgtgag
     1621 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     1681 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     1741 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     1801 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     1861 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     1921 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     1981 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     2041 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     2101 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     2161 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     2221 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     2281 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     2341 gattctgtgg ataaccgt
//
