LOCUS       Exported               11197 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Lentiviral expression of AXL C311F.
ACCESSION   .
VERSION     .
KEYWORDS    pHAGE-AXL-C311F
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11197)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11197)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11197
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6134..6158)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6211..6252
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6273..6840
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6290..6842
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6434..6451)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6661..6680
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6854..7573
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             6854..7570
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6899..6920)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7160..7179)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7507..7528
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7587..8175
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7640..7660)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8058..8069)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8250..8483
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8602..8620)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8688..8792
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8793..9653
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9011..9030)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9824..10412
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10313..10332
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10566..10583
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     10675..10694
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     protein_bind    10823..10844
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10859..10889
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10897..10913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10902..10924
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10921..10937
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10921..10937
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10987..11006
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg gcgtggcggt gccccaggat gggcagggtc
     3481 ccgctggcct ggtgcttggc gctgtgcggc tgggcgtgca tggcccccag gggcacgcag
     3541 gctgaagaaa gtcccttcgt gggcaaccca gggaatatca caggtgcccg gggactcacg
     3601 ggcacccttc ggtgtcagct ccaggttcag ggagagcccc ccgaggtaca ttggcttcgg
     3661 gatggacaga tcctggagct cgcggacagc acccagaccc aggtgcccct gggtgaggat
     3721 gaacaggatg actggatagt ggtcagccag ctcagaatca cctccctgca gctttccgac
     3781 acgggacagt accagtgttt ggtgtttctg ggacatcaga ccttcgtgtc ccagcctggc
     3841 tatgttgggc tggagggctt gccttacttc ctggaggagc ccgaagacag gactgtggcc
     3901 gccaacaccc ccttcaacct gagctgccaa gctcagggac ccccagagcc cgtggaccta
     3961 ctctggctcc aggatgctgt ccccctggcc acggctccag gtcacggccc ccagcgcagc
     4021 ctgcatgttc cagggctgaa caagacatcc tctttctcct gcgaagccca taacgccaag
     4081 ggggtcacca catcccgcac agccaccatc acagtgctcc cccagcagcc ccgtaacctc
     4141 cacctggtct cccgccaacc cacggagctg gaggtggctt ggactccagg cctgagcggc
     4201 atctaccccc tgacccactg caccctgcag gctgtgctgt cagacgatgg gatgggcatc
     4261 caggcgggag aaccagaccc cccagaggag cccctcacct cgcaagcatc cgtgcccccc
     4321 catcagcttc ggctaggcag cctccatcct cacacccctt atcacatccg cgtggcattc
     4381 accagcagcc agggcccctc atcctggacc cactggcttc ctgtggagac gccggaggga
     4441 gtgcccctgg gcccccctga gaacattagt gctacgcgga atgggagcca ggccttcgtg
     4501 cattggcaag agccccgggc gcccctgcag ggtaccctgt tagggtaccg gctggcgtat
     4561 caaggccagg acaccccaga ggtgctaatg gacatagggc taaggcaaga ggtgaccctg
     4621 gagctgcagg gggacgggtc tgtgtccaat ctgacagtgt gtgtggcagc ctacactgct
     4681 gctggggatg gaccctggag cctcccagta cccctggagg cctggcgccc agggcaagca
     4741 cagccagtcc accagctggt gaaggaacct tcaactcctg ccttctcgtg gccctggtgg
     4801 tatgtactgc taggagcagt cgtggccgct gcctgtgtcc tcatcttggc tctcttcctt
     4861 gtccaccggc gaaagaagga gacccgttat ggagaagtgt ttgaaccaac agtggaaaga
     4921 ggtgaactgg tagtcaggta ccgcgtgcgc aagtcctaca gtcgtcggac cactgaagct
     4981 accttgaaca gcttgggcat cagtgaagag ctgaaggaga agctgcggga tgtgatggtg
     5041 gaccggcaca aggtggccct ggggaagact ctgggagagg gagagtttgg agctgtgatg
     5101 gaaggccagc tcaaccagga cgactccatc ctcaaggtgg ctgtgaagac gatgaagatt
     5161 gccatctgca cgaggtcaga gctggaggat ttcctgagtg aagcggtctg catgaaggaa
     5221 tttgaccatc ccaacgtcat gaggctcatc ggtgtctgtt tccagggttc tgaacgagag
     5281 agcttcccag cacctgtggt catcttacct ttcatgaaac atggagacct acacagcttc
     5341 ctcctctatt cccggctcgg ggaccagcca gtgtacctgc ccactcagat gctagtgaag
     5401 ttcatggcag acatcgccag tggcatggag tatctgagta ccaagagatt catacaccgg
     5461 gacctggcgg ccaggaactg catgctgaat gagaacatgt ccgtgtgtgt ggcggacttc
     5521 gggctctcca agaagatcta caatggggac tactaccgcc agggacgtat cgccaagatg
     5581 ccagtcaagt ggattgccat tgagagtcta gctgaccgtg tctacaccag caagagcgat
     5641 gtgtggtcct tcggggtgac aatgtgggag attgccacaa gaggccaaac cccatatccg
     5701 ggcgtggaga acagcgagat ttatgactat ctgcgccggg gaaatcgcct gaagcagcct
     5761 gcggactgtc tggatggact gtatgccttg atgtcgcggt gctgggagct aaatccccag
     5821 gaccggccaa gttttacaga gctgcgggaa gatttggaga acacactgaa ggccttgcct
     5881 cctgcccagg agcctgacga aatcctctat gtcaacatgg atgagggtgg aggttatcct
     5941 gaaccccctg gagctgcagg aggagctgac cccccaaccc agccagaccc taaggattcc
     6001 tgtagctgcc tcactgcggc tgaggtccat cctgctggac gctatgtcct ctgcccttcc
     6061 acaaccccta gccccgctca gcctgctgat aggggctccc cagcagcccc agggcaggag
     6121 gatggtgcct agaacccagc tttcttgtac aaagtggttg atatccagca cagtggcggc
     6181 cgctcgagtc tagagggccc gcggttcgaa ggtaagccta tccctaaccc tctcctcggt
     6241 ctcgattcta cgcgtaccgg ttagtaatga gatccctccc ccccccctaa cgttactggc
     6301 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     6361 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     6421 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     6481 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     6541 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     6601 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     6661 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     6721 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     6781 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     6841 tatggccaca catatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     6901 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     6961 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     7021 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     7081 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     7141 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     7201 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     7261 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     7321 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     7381 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     7441 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     7501 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     7561 gctgtacaag taaatcgata gatcctaatc aacctctgga ttacaaaatt tgtgaaagat
     7621 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     7681 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     7741 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     7801 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     7861 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     7921 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     7981 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     8041 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     8101 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     8161 gggccgcctc cccgcctgag atcctttaag accaatgact tacaaggcag ctgtagatct
     8221 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     8281 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     8341 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     8401 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     8461 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     8521 acttgcaaag aaatgaatat cagagagtga gaggcccggg ttaattaagg aaagggctag
     8581 atcattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     8641 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     8701 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     8761 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     8821 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     8881 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     8941 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     9001 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg
     9061 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     9121 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     9181 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     9241 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     9301 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     9361 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     9421 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     9481 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     9541 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     9601 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     9661 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     9721 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     9781 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     9841 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     9901 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     9961 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    10021 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    10081 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    10141 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    10201 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    10261 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    10321 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    10381 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    10441 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
    10501 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
    10561 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
    10621 ccgcgcgttg gccgattcat taatgcagca agctcatggc tgactaattt tttttattta
    10681 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
    10741 tggaggccta ggcttttgca aaaagctccc cgtggcacga caggtttccc gactggaaag
    10801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
    10861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
    10921 caggaaacag ctatgacatg attacgaatt tcacaaataa agcatttttt tcactgcatt
    10981 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa
    11041 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt
    11101 acctgtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
    11161 attgtatttg ttaaatatgt actacaaact tagtagt
//
