LOCUS       Exported               11106 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  expresses Ustilago maydis codon-optimized Cas9, contains U. maydis 
            U6 promoter, is self-replicating.
ACCESSION   .
VERSION     .
KEYWORDS    pCas9_sgRNA_0
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11106)
  AUTHORS   Schuster M, Schweizer G, Reissmann S, Kahmann R
  TITLE     Genome editing in Ustilago maydis using the CRISPR-Cas system.
  JOURNAL   Fungal Genet Biol. 2015 Sep 11. pii: S1087-1845(15)30025-6. doi: 
            10.1016/j.fgb.2015.09.001.
  PUBMED    26365384
REFERENCE   2  (bases 1 to 11106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11106
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    1..271
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    308..578
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     CDS             857..877
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             881..4981
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4991..5017
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5027..5074
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      5095..5347
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(5377..5394)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5377..5393)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5386..5408)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5602..5621)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5721..5743
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5781..5799)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             8211..9071
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8429..8448)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9242..9830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9731..9750
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9984..10001
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10118..10139
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10154..10184
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10192..10208
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10197..10219
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10216..10232
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10216..10232
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tctctatcac tgatagggag tggtaaactc gactttcact tttctctatc actgataggg
       61 agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa actcgacttt
      121 cacttttctc tatcactgat agggagtggt aaactcgact ttcacttttc tctatcactg
      181 atagggagtg gtaaactcga ctttcacttt tctctatcac tgatagggag tggtaaactc
      241 gactttcact tttctctatc actgataggg agtggtaaac tcgagtaccg agctcgactt
      301 tcacttttct ctatcactga tagggagtgg taaactcgac tttcactttt ctctatcact
      361 gatagggagt ggtaaactcg actttcactt ttctctatca ctgataggga gtggtaaact
      421 cgactttcac ttttctctat cactgatagg gagtggtaaa ctcgactttc acttttctct
      481 atcactgata gggagtggta aactcgactt tcacttttct ctatcactga tagggagtgg
      541 taaactcgac tttcactttt ctctatcact gatagggagt ggtaaactcg aggggatcaa
      601 ttcgaccaat gaggcgcgag acgaggggac gctggaagtt gaggcgcaag aaaatttttc
      661 tctggttctg cgcggcagag acgaccagat tcgcccgctt tcttctgcgt tgggtgcctc
      721 ttttgggtgc cagactttgt gtgtgcgcca gcgagacgtt ccaataaagg gcgctgtctc
      781 ggcactatct ttctttcttt cctcatacat cgtatcatac catacacaga caacatcatc
      841 cacgggatcc atgccgccta agaagaaacg caaggttgag gataagaagt acagcatcgg
      901 actcgacatc ggtactaact cggtaggatg ggcagtcatc acggatgaat acaaggttcc
      961 ttccaagaag tttaaggtcc ttggtaacac cgaccgccat tctatcaaga agaacctcat
     1021 tggcgctttg ctctttgact caggagaaac cgctgaggcg acacgcctca aacgcacggc
     1081 acgtcgacgt tatacacgca gaaagaatcg tatctgctat ctgcaggaaa tcttttcgaa
     1141 cgaaatggca aaagttgatg acagcttctt ccatcgcctg gaggaatcgt ttctcgtgga
     1201 ggaggacaag aagcacgaga gacatcctat cttcggcaac attgtcgatg aggtcgctta
     1261 ccacgagaag taccctacta tctaccacct tagaaagaag ctcgtagact caactgacaa
     1321 agcggatctt cgtctgatct atttggctct tgcccacatg atcaagttcc gtggtcattt
     1381 tctcatcgaa ggcgacctta atcccgacaa ctcggacgtc gataagctgt ttatccagct
     1441 cgtacagaca tacaaccagc tcttcgaaga aaacccaatt aatgcttccg gtgttgatgc
     1501 aaaagctatc ctcagcgcga gattgagcaa gagcagacgc ctcgaaaacc tcatcgccca
     1561 attgcctggt gaaaagaaga acggcttgtt cggcaatctt attgccctga gccttggcct
     1621 cactccgaac ttcaagtcga acttcgatct tgcggaagat gccaagctgc aactctccaa
     1681 ggacacgtac gatgatgact tggacaatct ccttgcccag attggcgatc aatacgccga
     1741 tcttttcctc gcggcgaaga acttgtcgga cgcaatcttg ctctcagaca tccttcgcgt
     1801 caacactgag atcaccaaag cccctctctc tgcctcgatg atcaagcgct atgacgaaca
     1861 ccaccaggat ctcacgctcc ttaaggcatt ggtgcgtcag cagttgcctg agaagtacaa
     1921 agagattttc tttgatcagt cgaagaacgg atacgctggc tacatcgacg gtggcgcttc
     1981 tcaggaggag ttctacaagt ttatcaaacc cattcttgag aagatggatg gcacggagga
     2041 gctcctcgtc aagctgaatc gcgaggacct cctccgtaag caacgtacgt tcgacaatgg
     2101 ctcgattcca caccagattc atctgggcga actccacgcc atcctcagga ggcaggagga
     2161 cttctatccc ttcctcaagg ataatcgaga gaaaattgag aagatcctca cattccgcat
     2221 cccctattat gtaggcccac tcgctcgcgg aaactctcgc tttgcctgga tgacccgcaa
     2281 gtcggaagaa acaatcaccc cgtggaactt cgaagaggtg gtggacaagg gtgcatctgc
     2341 gcagtcgttt attgagagga tgacaaactt tgataagaac ctcccgaatg agaaagtcct
     2401 gccaaaacat tccctcctgt atgaatactt cacggtctat aacgaactga caaaggtgaa
     2461 gtacgtgacc gagggtatgc gtaagcctgc ctttctttcg ggtgagcaga agaaagctat
     2521 tgtcgacttg ttgttcaaga ccaaccgcaa ggtcactgtc aagcaactga aggaagatta
     2581 cttcaagaaa atcgagtgtt ttgattcggt agagatctcg ggcgtcgagg acaggttcaa
     2641 cgcctctctc ggcacctatc acgatcttct caagatcatc aaggacaaag actttcttga
     2701 caacgaagag aacgaggata ttctcgagga catcgtgctc accctcactt tgttcgaaga
     2761 tcgcgaaatg attgaggaac gtcttaagac atatgctcac ttgttcgacg acaaagtgat
     2821 gaagcagctg aagcgtaggc gatacacagg ttggggccgc ctctcgcgca agctgattaa
     2881 cggtatccgc gacaagcaat ccggcaagac aatcttggat ttccttaaga gcgacggttt
     2941 tgctaaccgc aacttcatgc agctcatcca cgacgacagc cttacgttca aggaggacat
     3001 ccagaaggcc caggtttccg gacaaggtga ctctctccat gagcacatcg ctaacctggc
     3061 gggaagcccc gcgatcaaga aaggtatcct ccagaccgtc aaagttgtgg acgagctggt
     3121 caaggtaatg ggccgacaca aaccggagaa cattgttatc gagatggcac gagagaatca
     3181 gacgacccag aaaggccaaa agaactccag agaacgtatg aaacgaatcg aagagggtat
     3241 caaggaactg ggatcgcaga tcctgaagga gcaccccgtt gagaacacgc agctccaaaa
     3301 cgaaaagctg tacctctact acttgcaaaa tggtagggat atgtacgtcg accaggaact
     3361 ggatattaat cgtctgtccg actacgacgt tgaccacatc gtgccccaat cgtttctcaa
     3421 ggatgactcg atcgataata aagtacttac gcgctcagac aagaaccgag gtaaatcgga
     3481 caatgtccca tcggaggaag tcgtgaagaa gatgaagaac tattggcgcc aacttcttaa
     3541 cgcaaagctg atcacccaga ggaaattcga caacctcacc aaagcagaac gcggcggcct
     3601 ctccgagctc gacaaggctg gatttatcaa gcgtcagctc gtcgaaacgc gtcagattac
     3661 caagcacgtc gcacagatcc tggatagccg catgaacaca aagtacgacg aaaacgacaa
     3721 gctcatccgt gaggttaagg tcatcacctt gaagtcgaaa ctcgtgtcgg acttccgcaa
     3781 agattttcag ttctataaag ttagagagat caacaactac caccatgcgc atgacgccta
     3841 cctcaatgcc gtcgtgggca ccgcacttat taagaaatac ccgaagctcg agtccgagtt
     3901 tgtctacggc gattacaagg tatacgacgt tcgcaagatg attgccaaat cggagcagga
     3961 gatcggtaag gccactgcca agtacttctt ttactcgaac atcatgaatt tcttcaaaac
     4021 agaaatcacc ctcgccaacg gcgagattcg caaacgacca ctcatcgaga ctaacggtga
     4081 aacgggagag atcgtctggg ataagggccg agactttgct acggttcgaa aggtcctttc
     4141 gatgcctcaa gtgaacatcg tcaagaaaac ggaggtccaa accggtggct tcagcaagga
     4201 gtcgattctg ccgaaacgca attcggacaa attgattgca cgcaagaagg attgggaccc
     4261 taagaaatat ggcggcttcg attcaccgac agtggcctat tcggttctgg tcgtcgcgaa
     4321 agtggagaag ggcaagtcaa agaagctcaa gtcagtgaag gagctcctgg gaatcaccat
     4381 catggaacgt tcctcttttg agaagaaccc tatcgacttt ctcgaggcta agggctacaa
     4441 agaggtcaag aaagatctca tcatcaaact cccaaaatac tcacttttcg agctcgagaa
     4501 cggccgtaaa cgaatgctgg cgagcgcagg agagcttcaa aagggaaatg aactggcttt
     4561 gccctccaag tacgtcaact tcctctacct cgcaagccat tatgagaagt tgaagggtag
     4621 ccccgaggac aacgaacaaa agcagctctt cgtggagcaa cacaaacatt acctggatga
     4681 aatcatcgag caaatctcgg agtttagcaa gcgagtgatc ttggctgatg ccaacctcga
     4741 caaggtgttg tctgcctaca acaagcatcg agataagccg attcgcgagc aggccgagaa
     4801 catcatccac ctcttcactc tcactaactt gggtgcgcct gcggcgttta aatactttga
     4861 cacgaccatc gaccgcaagc gttacacaag cacgaaggaa gtcctcgacg ctacactgat
     4921 ccatcagtcg atcaccggtc tgtacgaaac ccgcatcgat ctgtctcaac tgggcggtga
     4981 cagcggcggc tacccatacg atgtgcccga ttacgctagc ggcggaaagc gtcccgcagc
     5041 cactaagaag gctggacagg ccaagaagaa gaagtgagcg gccgcccggc tgcagatcgt
     5101 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
     5161 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
     5221 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
     5281 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
     5341 ctagatccga tgataagctg tcaaacatga gaattcactg gccgtcgttt tacaacgtcg
     5401 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     5461 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     5521 gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     5581 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     5641 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     5701 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     5761 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     5821 aataatggtt tcttaggcgg ccgcacgcta agtggagttg tccgagtccc gaatcacaag
     5881 aattaggctc gtgctctgtg agatctctcg cgaaacccag atgaaggaaa aaaatcggaa
     5941 gatcgcggaa gaagtggggt tcgcatggtc taacattgtc gcattcctca cagtttcggc
     6001 tggaaacggc agggacaatc acgagaaatg cgcgacgagt attcagttgt ccaaaattat
     6061 gtcagcttga aagttggaaa cggcggaaga aaattcgtca ggagggcgtt gcggctaggg
     6121 tgaagaacgg agacaatttc ggctttgaaa ttggctcgac tctgattgga tcatgacgca
     6181 ctgctggtcc acaaccgtgt cgaaggtgcc gtctttacta caggtccgct ggaagcaaaa
     6241 tggaaaaagc cgctggagcc cgactacaga gccgccgtgt tttggtaatc agtcggcaaa
     6301 taggtcagca cagcgcagcg tgacaggttc ttgcaattta cagcacagct cgtccgtcta
     6361 cgactttgca caccacaaag tgtgcgggga gcaaaggagc cgatcttggt cgcgcgcaaa
     6421 gccaaggagt cttgaacctg agagtgtgcg tgtcttgtga cgcttgccct tctgtacttt
     6481 gctgtgacac taccaccaca tctgtcttgg ctttttgttc atacatccac accgaccatg
     6541 tcgctattca acgtcagcaa cggtcttcgt accgctctcc gaccttctgt tgccagctct
     6601 tcgcgcgttg ctgccttttc cacaaccgcc gctgcccgtc tcgccacacc cacctctgac
     6661 aacgttggca gttcgggcaa gcctcagcac ttgaagcagt tcaagatcta ccgatggaac
     6721 cctgacaagc cctcggagaa gcctcgtctg cagtcgtaca cactggacct caaccagacc
     6781 ggtccaatgg ttctcgacgc gctcatcaaa atcaagaacg aaattgaccc tacgctcacc
     6841 ttccgtcgct cgtgccgtga gggtatctgc ggttcgtgcg ctatgaatat tgacggtgtc
     6901 aacaccctcg cctgcctctg ccggatcgac aagcagaatg acaccaagat ctaccccttg
     6961 ccgcacatgt acattgtcaa ggacctcgtg ccagacttga cccagttcta caagcagtac
     7021 cgatccatcg agcctttcct caagtccaac aacacccctt ctgagggtga acatcttcag
     7081 tcgcccgagg agcgtcgtcg actcgacggt ctgtacgagt gcattctgtg cgcgtgctgc
     7141 tccacatcct gcccctctta ctggtggaat caggacgagt accttggccc cgccgtgctc
     7201 atgcaggcgt accgatggat ggccgactcg cgtgacgact ttggtgagga gcgaagacag
     7261 aagctcgaga acaccttttc gctctaccga tgccttacca tcatgaactg ctccaggacc
     7321 tgccccaaga acctcaaccc tggtaaggca attgcacaga tcaagaagga catggccgtc
     7381 ggcgcaccca aggcttccga gcgccctatc atggcttcgt cgtaatcttg atatatcata
     7441 tcgttctttc ctcagcactt cttttgtcaa tttcaaaagt atctaattgc attcaactcc
     7501 gcttgtggtt tgttgttcag tgagagtgga aacgctacgg gcaagatgag ggcagtgttc
     7561 tggcgacgga aaagtgtgca agtgtctggc ctgcgtcctc gctggttcca gcagccgatg
     7621 caggacgtgt acctagcgat ttcttcgaca gcctattgtg gcagccgcga ttcgccacaa
     7681 tcgtacgtgc ggccgccagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     7741 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     7801 cttcaataat attaccgatc ctcgatcttt gtgcaagcta gcccgcctcg gcagcaacaa
     7861 agcagccgag caagaagcag tacttgcctt ctgaatcgtg aatgggttac gttcttcacc
     7921 gctgtgatca gcgaatcatg aatcaaatca tgagggcatt gctgatcatg aatcaaatca
     7981 tgagggcatt taaaaattca gtctgagtcg tgagtagcaa gtcggttctg gatcggatgg
     8041 cattcatgaa tcacagggtc gtgaatcatg aatgttcaag tccccttttc tcgagaggct
     8101 ggtggcatcg gtgcgaatca cgaatcatga ttgtaattca ttgagtgaag gagtttcgca
     8161 gccacccaca gtactagaat cacgaatgac aatattgaaa aaggaagagt atgagtattc
     8221 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     8281 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     8341 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     8401 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     8461 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     8521 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     8581 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     8641 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     8701 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     8761 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     8821 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     8881 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     8941 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     9001 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     9061 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     9121 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     9181 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     9241 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     9301 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     9361 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     9421 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     9481 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     9541 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     9601 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     9661 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     9721 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     9781 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     9841 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     9901 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     9961 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
    10021 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
    10081 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
    10141 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
    10201 gataacaatt tcacacagga aacagctatg accatgatta cgccaagctt taatacgttc
    10261 gttccgatgt agctcaggaa cgggcgcaga ggcggatgaa ttggttgcgg tggctatgga
    10321 tggtggaggt gaacggcgac gtgcagcgtc ggtcacagag gatcggatgg ggcctgtcgg
    10381 gatgacctgt actataacga gggaggaggg ggagggagga ggggaaagag gaatgtgggg
    10441 aaggcacgtt accaaggttt tgccaagagg ctcttgtgtt attccggagg tgtagccgaa
    10501 tgggcgatcc gagctaacgc cagctgggcg tgagaagcag ttggtctgaa agcggaggcg
    10561 gtgcaagacg gttctaagaa ggaggcgaat aagaagtgtt ttgtgtgctg cgggtgcgaa
    10621 tagacggtca cgagtggatg gaagccgact tgtaggcgtg ctgaaagacg tcgtgcgggt
    10681 gcggttttgg ttttgtgttg gtcttggtaa aagtgtgccg caggtgaggg ttcttgattg
    10741 gtgaacgtga aaacggatgg ccaagtccga gtcgaccaga gagagaggca gagagagaga
    10801 gagagatggc tcttcagcgc cgcttcgcgc gtccttcacg attattcgtg attactgtcc
    10861 accggtccct cttactcaga actgccggaa cgaattcgtg atttacacca aacacgcgct
    10921 gtcacagtca cgagtccatg agccgtgagc cccgcttcag atcctgtttt ctcttattca
    10981 gcgtaacaac acaaaacaga atttcttcta aacacccttg caattcgcgc acacccctgt
    11041 agcagtctgt cagcattcaa aattccattc tacaacggta cccgtaccga gctcgacttt
    11101 cacttt
//
