LOCUS Exported 7718 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina. ACCESSION . VERSION . KEYWORDS 6_pAAV-ProA37-CatCh-GFP-WPRE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7718) AUTHORS Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B TITLE Targeting neuronal and glial cell types with synthetic promoter AAVs in mice, non-human primates and humans. JOURNAL Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 10.1038/s41593-019-0431-2. PUBMED 31285614 REFERENCE 2 (bases 1 to 7718) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7718 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..141 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 12..141 /label=AAV2 ITR regulatory 2162..2171 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 2168..3091 /codon_start=1 /product="N-terminal fragment of channelrhodopsin-2 from the alga Chlamydomonas reinhardtii (Nagel et al., 2003)" /label=ChR2 /note="blue light-activated cation channel" /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI RKTTKLNIGGTEIEVETLVEDEAEAGA" CDS 3098..3814 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(3143..3164) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(3404..3423) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 3751..3772 /label=EGFP-C /note="EGFP, forward primer" misc_feature 3845..4433 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(3898..3918) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(4316..4327) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 4465..4941 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(4664..4683) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" repeat_region 4981..5121 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 4981..5110 /label=AAV2 ITR rep_origin 5196..5651 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5283..5302) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5493..5514 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(5668..5687) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 5787..5809 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(5847..5865) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 5933..6037 /gene="bla" /label=AmpR promoter CDS 6038..6898 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(6256..6275) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 7069..7657 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7558..7577 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtgagcc gagttagctc tcttacagct 181 gtgtggttcc attgtcatta ataagtgcga gtctctccta cttcctctgt gacttttgct 241 tcttactcat tttacaagat caccacgtgt cttccctggg ttctaagcct taattggtca 301 ctggtgtgta cagggcactt ctcaacctgt gggtcacgat ccttccacag gggccacata 361 tcagatatcc tgcctatcag gtatttacga tacgattcct aacagtagca ctattacagg 421 tatgaagtag cagtgaaaag aattttatgg ttgggggtcg ccaccacatg aggagccgta 481 ttaggaaggt tgagaacggc tggtctaagg catatagctg atgatgtcaa aagacatatc 541 atccatgggg gacagctgtt gctccttccg tcttctcctt cgctgggtcc tgggcccttg 601 gcttccagca ggctgtgagg taaatgtggg atatgaagct gtatcctgag tcaattttac 661 ctgacaagcc aagggaagga agcagatggt caggaagggg ccagccctca ggagacttcc 721 ttcctcccca gctaggctac acctcatcat ttacttcagc ctccccagat agcgccacca 781 gccgggggga catgcaagct agagggtgac atttcatgcc caaacccagg gaagtgagtc 841 gcccagctag ctgaggccag tgtgtgctag cgttctgctt gcttctccca ctctccacac 901 agtggcctct ctacagggca cctctacagg gcgtccaact cagccggcag cacattgcct 961 ggcctcggct ggcctccatg atacctcatt cttgcatttc cacgtgaaca tcgagttttg 1021 gggactgccc cctctgcttt aggatgctgc ccatcagcag aactattctg gtcaaagtgt 1081 gatcctaccc atcagaccct cccgtgtttc aggcggtctg aggctttgtc agaactctgt 1141 ggaatctccc tctgggaggc aggctctccc tccgattgat tgatggctcc cagccatctc 1201 cttctctcac cttgagactt gggatggggt gaagtcgggg gagtcccagt ttcttggtcc 1261 actgtttcaa gagtctgttg gctagaggga agggcacaag cttctcttca gagaaatctt 1321 tattcctgca gccaggttca tccccgcact gccccttggc tgtaaaatcc ccagcccttg 1381 tttcagtaca aagtccagtc tctctgcctt aacttagtag agaaaatgct ctggttagaa 1441 cagtcttgaa taaatatgac attttagcag cttgacacac caaacaggtg cctaaggcgc 1501 ccaaggtacc ttgatggtct tgtctttgtt gttcaagatg cggtagcctt cggaagagat 1561 acctggcagt gcaagtccac attaggacgc agggaaaaga ggggcgtgtg ggtggggggt 1621 gcggggggcg ggtgtctacg aatagctgca gcccaggatt ggccctgtag atcctcaagg 1681 ctggtcagat cttttccctt catatcccag ctggttggaa gcaccctggc tgctgccaaa 1741 ctgctcccca cagaccccag aggggcggct gcccgcagcc tggtttctaa tttcaaaatc 1801 acgccacgtt ctgcaaacgt tgtgtgtttt aggtcaaata aacaactctg caaacatgta 1861 aataagctgc tctgcactga ttgatcctgg gagagggtca gcgagggagg gaaggggagg 1921 gagggaggga gggaccgaag ggggggaagg cagcggaaag taggggcagg cggtgagatt 1981 ccacagctga gccaccacct cccgcccagg ccgaatgaag atgcacaatt tggattggag 2041 gggaagggtc aggagctgct gacctttggg ctcaggccca ggccgctggc cacagtagct 2101 cttggtccag tcggcgccgg aggtgtatca agcgctcatc ccgccctctc cagtcggatc 2161 cgccaccatg gattatggag gcgccctgag tgccgttggg cgcgagctgc tatttgtaac 2221 gaacccagta gtcgtcaatg gctctgtact tgtgcctgag gaccagtgtt actgcgcggg 2281 ctggattgag tcgcgtggca caaacggtgc ccaaacggcg tcgaacgtgc tgcaatggct 2341 tgctgctggc ttctccatcc tactgcttat gttttacgcc taccaaacat ggaagtcaac 2401 ctgcggctgg gaggagatct atgtgtgcgc tatcgagatg gtcaaggtga ttctcgagtt 2461 cttcttcgag tttaagaacc cgtccatgct gtatctagcc acaggccacc gcgtccagtg 2521 gttgcgttac gccgagtggc ttctcacctg cccggtcatt tgcattcacc tgtcaaacct 2581 gacgggcttg tccaacgact acagcaggcg caccatgggt ctgcttgtgt ctgatattgg 2641 cacaattgtg tggggcgcca cttccgccat ggccaccgga tacgtcaagg tcatcttctt 2701 ctgcctgggt ctgtgttatg gtgctaacac gttctttcac gctgccaagg cctacatcga 2761 gggttaccac accgtgccga agggccggtg tcgccaggtg gtgactggca tggcttggct 2821 cttcttcgta tcatggggta tgttccccat cctgttcatc ctcggccccg agggcttcgg 2881 cgtcctgagc gtgtacggct ccaccgtcgg ccacaccatc attgacctga tgtcgaagaa 2941 ctgctggggt ctgctcggcc actacctgcg cgtgctgatc cacgagcata tcctcatcca 3001 cggcgacatt cgcaagacca ccaaattgaa cattggtggc actgagattg aggtcgagac 3061 gctggtggag gacgaggccg aggctggcgc ggtacccatg gtgagcaagg gcgaggagct 3121 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt 3181 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat 3241 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg 3301 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc 3361 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa 3421 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg 3481 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag 3541 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat 3601 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc 3661 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct 3721 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc 3781 cgggatcact ctcggcatgg acgagctgta caagtgataa gaattcgata tcaagcttat 3841 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt 3901 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc 3961 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga 4021 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc 4081 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct 4141 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg 4201 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct 4261 gctcgcctat gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc 4321 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg 4381 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata 4441 ccgagcgctg ctcgagagat ctacgggtgg catccctgtg acccctcccc agtgcctctc 4501 ctggccctgg aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc 4561 atcattttgt ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg 4621 agcaaggggc aagttgggaa gacaacctgt agggcctgcg gggtctattg ggaaccaagc 4681 tggagtgcag tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt 4741 ctcctgcctc agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa 4801 tttttgtttt tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc 4861 taatctcagg tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc 4921 actgctccct tccctgtcct tctgattttg taggtaacca cgtgcggacc gagcggccgc 4981 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg 5041 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc 5101 gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg 5161 gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag 5221 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc 5281 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc 5341 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa 5401 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg 5461 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac 5521 actcaaccct atctcgggct attcttttga tttataaggg attttgccga tttcggccta 5581 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac 5641 gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca 5701 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc 5761 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc 5821 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt 5881 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac 5941 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc 6001 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt 6061 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct 6121 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga 6181 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag 6241 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca 6301 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga 6361 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag 6421 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc 6481 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa 6541 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt 6601 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg 6661 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt 6721 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg 6781 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat 6841 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact 6901 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa 6961 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 7021 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 7081 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 7141 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 7201 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt 7261 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 7321 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 7381 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 7441 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 7501 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 7561 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 7621 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 7681 acggttcctg gccttttgct ggccttttgc tcacatgt //