LOCUS Exported 6483 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina. ACCESSION . VERSION . KEYWORDS 8_pAAV-ProC4-CatCh-GFP-WPRE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6483) AUTHORS Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B TITLE Targeting neuronal and glial cell types with synthetic promoter AAVs in mice, non-human primates and humans. JOURNAL Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 10.1038/s41593-019-0431-2. PUBMED 31285614 REFERENCE 2 (bases 1 to 6483) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6483 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..141 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 12..141 /label=AAV2 ITR promoter 724..920 /label=SV40 promoter /note="SV40 early promoter" rep_origin 771..906 /label=SV40 ori /note="SV40 origin of replication" primer_bind 833..852 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" regulatory 927..936 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 933..1856 /codon_start=1 /product="N-terminal fragment of channelrhodopsin-2 from the alga Chlamydomonas reinhardtii (Nagel et al., 2003)" /label=ChR2 /note="blue light-activated cation channel" /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI RKTTKLNIGGTEIEVETLVEDEAEAGA" CDS 1863..2579 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(1908..1929) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(2169..2188) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 2516..2537 /label=EGFP-C /note="EGFP, forward primer" misc_feature 2610..3198 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2663..2683) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(3081..3092) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 3230..3706 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(3429..3448) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" repeat_region 3746..3886 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 3746..3875 /label=AAV2 ITR rep_origin 3961..4416 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4048..4067) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4258..4279 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(4433..4452) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 4552..4574 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(4612..4630) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 4698..4802 /gene="bla" /label=AmpR promoter CDS 4803..5663 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5021..5040) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5834..6422 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6323..6342 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgccgctg gtggaaaata tttaattaaa 181 ggtccgcatt agattaatta taaccacctg taaagttaat tatagagtcc tccgccttta 241 attaactggg aacgagggtt taattacccc tgcgttttac gttaattata ttgacggcta 301 tgcttaatta tggcgcactg tgtgtttaat taaccacctg caatttctta attagcgatt 361 ttgggatttt taattaaaga tgggatttgc gttaattaaa attcaagtat ggattaatta 421 tgaaagtcta agggtttaat tatcgtactt ttctatatta attaagatgt gtaaggtcgt 481 taattaatcg ttcgaattca cttaattatc aagggcaatt ttattaatta ccagtgaggt 541 gttacttaat tacgcgttac aggacgttta attactaaat cgacatcggt taattattca 601 taagcaagat gttaattatg ctaaagcgca cttttaatta aaaccctgca atagcttaat 661 tatgctttac ttcaggatta attagtggac caatcctttt taattagctc gagatctgcg 721 atctgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc 781 taactccgcc cagttccgcc cattctccgc cccatcgctg actaattttt tttatttatg 841 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 901 gaggcctagg cttttgcaaa ggatccgcca ccatggatta tggaggcgcc ctgagtgccg 961 ttgggcgcga gctgctattt gtaacgaacc cagtagtcgt caatggctct gtacttgtgc 1021 ctgaggacca gtgttactgc gcgggctgga ttgagtcgcg tggcacaaac ggtgcccaaa 1081 cggcgtcgaa cgtgctgcaa tggcttgctg ctggcttctc catcctactg cttatgtttt 1141 acgcctacca aacatggaag tcaacctgcg gctgggagga gatctatgtg tgcgctatcg 1201 agatggtcaa ggtgattctc gagttcttct tcgagtttaa gaacccgtcc atgctgtatc 1261 tagccacagg ccaccgcgtc cagtggttgc gttacgccga gtggcttctc acctgcccgg 1321 tcatttgcat tcacctgtca aacctgacgg gcttgtccaa cgactacagc aggcgcacca 1381 tgggtctgct tgtgtctgat attggcacaa ttgtgtgggg cgccacttcc gccatggcca 1441 ccggatacgt caaggtcatc ttcttctgcc tgggtctgtg ttatggtgct aacacgttct 1501 ttcacgctgc caaggcctac atcgagggtt accacaccgt gccgaagggc cggtgtcgcc 1561 aggtggtgac tggcatggct tggctcttct tcgtatcatg gggtatgttc cccatcctgt 1621 tcatcctcgg ccccgagggc ttcggcgtcc tgagcgtgta cggctccacc gtcggccaca 1681 ccatcattga cctgatgtcg aagaactgct ggggtctgct cggccactac ctgcgcgtgc 1741 tgatccacga gcatatcctc atccacggcg acattcgcaa gaccaccaaa ttgaacattg 1801 gtggcactga gattgaggtc gagacgctgg tggaggacga ggccgaggct ggcgcggtac 1861 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg 1921 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct 1981 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca 2041 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga 2101 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct 2161 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc 2221 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc 2281 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga 2341 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg 2401 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc 2461 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg 2521 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt 2581 gataagaatt cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa 2641 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa 2701 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat 2761 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt 2821 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc 2881 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc 2941 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg 3001 ggaaatcatc gtcctttcct tggctgctcg cctatgttgc cacctggatt ctgcgcggga 3061 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc 3121 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc 3181 tttgggccgc ctccccgcat cgataccgag cgctgctcga gagatctacg ggtggcatcc 3241 ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag tgcccaccag 3301 ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct tctataatat 3361 tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa cctgtagggc 3421 ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc tcactgcaat 3481 ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt tgggattcca 3541 ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg gtttcaccat 3601 attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt ggcctcccaa 3661 attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctga ttttgtaggt 3721 aaccacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct 3781 ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt 3841 gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg 3901 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt 3961 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 4021 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 4081 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 4141 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc 4201 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 4261 gactcttgtt ccaaactgga acaacactca accctatctc gggctattct tttgatttat 4321 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 4381 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct 4441 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct 4501 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct 4561 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga 4621 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca 4681 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata 4741 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga 4801 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc 4861 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg 4921 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc 4981 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat 5041 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact 5101 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat 5161 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga 5221 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc 5281 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga 5341 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag 5401 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc 5461 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt 5521 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct 5581 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg 5641 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg 5701 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca 5761 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga 5821 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 5881 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 5941 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt 6001 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 6061 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 6121 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 6181 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 6241 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 6301 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 6361 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 6421 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 6481 tgt //