LOCUS       Exported                6521 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    35_pAAV-ProC8-CatCh-GFP-WPRE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6521
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        762..958
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      809..944
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     871..890
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     regulatory      965..974
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             971..1894
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGA"
     CDS             1901..2617
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1946..1967)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2207..2226)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2554..2575
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2648..3236
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2701..2721)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3119..3130)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3268..3744
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3467..3486)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3784..3924
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3784..3913
                     /label=AAV2 ITR
     rep_origin      3999..4454
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4086..4105)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4296..4317
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4471..4490)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4590..4612
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4650..4668)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4736..4840
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4841..5701
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5059..5078)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5872..6460
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6361..6380
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgaatc cgcgatagca ccgcctgagg ggattgagga
      181 ctccgtcaga ccgcctgagg ggatggaaga aagataatca ccgcctgagg ggatggctcg
      241 gttttgcaca ccgcctgagg ggatacgaaa ttctatcgca ccgcctgagg ggataagctc
      301 gttcctatga ccgcctgagg ggataatctc ctcaatacga ccgcctgagg ggattataga
      361 atgcattcaa ccgcctgagg ggatgaacta ccggttttca ccgcctgagg ggattgagct
      421 cccacggcta ccgcctgagg ggataaggcc agaactgtca ccgcctgagg ggattctgac
      481 taattcggga ccgcctgagg ggatagtttc ggtctggata ccgcctgagg ggataacctc
      541 tccgcggtga ccgcctgagg ggatgcgcca tggcggttaa ccgcctgagg ggatattccg
      601 ccttctacaa ccgcctgagg ggataaagag cgagccgaga ccgcctgagg ggatcatcca
      661 cgggtctcta ccgcctgagg ggattcccct acgagttgga ccgcctgagg ggattctaac
      721 tcagtggcaa ccgcctgagg ggatgctcga gatctgcgat ctgcatctca attagtcagc
      781 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
      841 ttctccgccc catcgctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
      901 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaagg
      961 atccgccacc atggattatg gaggcgccct gagtgccgtt gggcgcgagc tgctatttgt
     1021 aacgaaccca gtagtcgtca atggctctgt acttgtgcct gaggaccagt gttactgcgc
     1081 gggctggatt gagtcgcgtg gcacaaacgg tgcccaaacg gcgtcgaacg tgctgcaatg
     1141 gcttgctgct ggcttctcca tcctactgct tatgttttac gcctaccaaa catggaagtc
     1201 aacctgcggc tgggaggaga tctatgtgtg cgctatcgag atggtcaagg tgattctcga
     1261 gttcttcttc gagtttaaga acccgtccat gctgtatcta gccacaggcc accgcgtcca
     1321 gtggttgcgt tacgccgagt ggcttctcac ctgcccggtc atttgcattc acctgtcaaa
     1381 cctgacgggc ttgtccaacg actacagcag gcgcaccatg ggtctgcttg tgtctgatat
     1441 tggcacaatt gtgtggggcg ccacttccgc catggccacc ggatacgtca aggtcatctt
     1501 cttctgcctg ggtctgtgtt atggtgctaa cacgttcttt cacgctgcca aggcctacat
     1561 cgagggttac cacaccgtgc cgaagggccg gtgtcgccag gtggtgactg gcatggcttg
     1621 gctcttcttc gtatcatggg gtatgttccc catcctgttc atcctcggcc ccgagggctt
     1681 cggcgtcctg agcgtgtacg gctccaccgt cggccacacc atcattgacc tgatgtcgaa
     1741 gaactgctgg ggtctgctcg gccactacct gcgcgtgctg atccacgagc atatcctcat
     1801 ccacggcgac attcgcaaga ccaccaaatt gaacattggt ggcactgaga ttgaggtcga
     1861 gacgctggtg gaggacgagg ccgaggctgg cgcggtaccc atggtgagca agggcgagga
     1921 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     1981 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     2041 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     2101 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     2161 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     2221 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     2281 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     2341 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     2401 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     2461 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     2521 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     2581 cgccgggatc actctcggca tggacgagct gtacaagtga taagaattcg atatcaagct
     2641 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     2701 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     2761 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     2821 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     2881 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     2941 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     3001 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     3061 gctgctcgcc tatgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     3121 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     3181 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     3241 ataccgagcg ctgctcgaga gatctacggg tggcatccct gtgacccctc cccagtgcct
     3301 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt
     3361 tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta
     3421 tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca
     3481 agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg
     3541 attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc
     3601 taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac
     3661 tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga
     3721 accactgctc ccttccctgt ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc
     3781 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     3841 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
     3901 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt
     3961 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
     4021 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     4081 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     4141 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     4201 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     4261 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     4321 aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc
     4381 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     4441 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     4501 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     4561 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     4621 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     4681 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     4741 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     4801 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     4861 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     4921 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     4981 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     5041 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     5101 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     5161 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     5221 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     5281 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     5341 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     5401 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     5461 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     5521 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     5581 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     5641 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     5701 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     5761 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     5821 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     5881 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     5941 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6001 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6061 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     6121 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6181 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     6241 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     6301 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     6361 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     6421 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     6481 tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
