LOCUS       Exported                6421 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    37_pAAV-ProC40-CatCh-GFP-WPRE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6421)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6421
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        662..858
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      709..844
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     771..790
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     regulatory      865..874
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             871..1794
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGA"
     CDS             1801..2517
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1846..1867)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2107..2126)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2454..2475
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2548..3136
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2601..2621)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3019..3030)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3168..3644
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3367..3386)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3684..3824
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3684..3813
                     /label=AAV2 ITR
     rep_origin      3899..4354
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3986..4005)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4196..4217
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4371..4390)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4490..4512
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4550..4568)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4636..4740
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4741..5601
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4959..4978)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5772..6360
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6261..6280
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtatggtc tcatattctg ctaattagca gcgtgaggcc
      181 taatgctaat tagccaggtc cgtctcccgg ctaattagca tcaccctcat gattgctaat
      241 tagcagtcct cgtagagtag ctaattagcg cgtgctcgtg atgggctaat tagcaggacg
      301 aaggcgaccg ctaattagcc gcgccctaga tacagctaat tagccttatc acgtcggcgg
      361 ctaattagct gatgcttctc tatagctaat tagcagccct gaaagttccg ctaattagcg
      421 acgagtgcct aacagctaat tagctaatac aagtttcgtg ctaattagcc gaccttcgag
      481 ttaagctaat tagccgcgca tcaggcaaag ctaattagct tatctccatt acatgctaat
      541 tagcgttatg ttttacggcg ctaattagcg attcgagaaa atgagctaat tagccctccg
      601 ttcgtccacg ctaattagct gtgccttcta gaacgctaat tagcgctcga gatctgcgat
      661 ctgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
      721 actccgccca gttccgccca ttctccgccc catcgctgac taattttttt tatttatgca
      781 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
      841 ggcctaggct tttgcaaagg atccgccacc atggattatg gaggcgccct gagtgccgtt
      901 gggcgcgagc tgctatttgt aacgaaccca gtagtcgtca atggctctgt acttgtgcct
      961 gaggaccagt gttactgcgc gggctggatt gagtcgcgtg gcacaaacgg tgcccaaacg
     1021 gcgtcgaacg tgctgcaatg gcttgctgct ggcttctcca tcctactgct tatgttttac
     1081 gcctaccaaa catggaagtc aacctgcggc tgggaggaga tctatgtgtg cgctatcgag
     1141 atggtcaagg tgattctcga gttcttcttc gagtttaaga acccgtccat gctgtatcta
     1201 gccacaggcc accgcgtcca gtggttgcgt tacgccgagt ggcttctcac ctgcccggtc
     1261 atttgcattc acctgtcaaa cctgacgggc ttgtccaacg actacagcag gcgcaccatg
     1321 ggtctgcttg tgtctgatat tggcacaatt gtgtggggcg ccacttccgc catggccacc
     1381 ggatacgtca aggtcatctt cttctgcctg ggtctgtgtt atggtgctaa cacgttcttt
     1441 cacgctgcca aggcctacat cgagggttac cacaccgtgc cgaagggccg gtgtcgccag
     1501 gtggtgactg gcatggcttg gctcttcttc gtatcatggg gtatgttccc catcctgttc
     1561 atcctcggcc ccgagggctt cggcgtcctg agcgtgtacg gctccaccgt cggccacacc
     1621 atcattgacc tgatgtcgaa gaactgctgg ggtctgctcg gccactacct gcgcgtgctg
     1681 atccacgagc atatcctcat ccacggcgac attcgcaaga ccaccaaatt gaacattggt
     1741 ggcactgaga ttgaggtcga gacgctggtg gaggacgagg ccgaggctgg cgcggtaccc
     1801 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     1861 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     1921 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     1981 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     2041 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     2101 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     2161 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     2221 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     2281 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     2341 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     2401 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     2461 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtga
     2521 taagaattcg atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
     2581 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     2641 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     2701 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     2761 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     2821 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     2881 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     2941 aaatcatcgt cctttccttg gctgctcgcc tatgttgcca cctggattct gcgcgggacg
     3001 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     3061 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     3121 tgggccgcct ccccgcatcg ataccgagcg ctgctcgaga gatctacggg tggcatccct
     3181 gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc
     3241 ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta
     3301 tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct
     3361 gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct
     3421 ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg
     3481 catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat
     3541 tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat
     3601 tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt ttgtaggtaa
     3661 ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct
     3721 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
     3781 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta
     3841 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
     3901 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     3961 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4021 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4081 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
     4141 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4201 ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa
     4261 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4321 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
     4381 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     4441 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     4501 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     4561 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     4621 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4681 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4741 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4801 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4861 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4921 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     4981 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     5041 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5101 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     5161 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     5221 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5281 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5341 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5401 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     5461 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     5521 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     5581 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     5641 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5701 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5761 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5821 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5881 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     5941 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6001 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6061 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6121 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6181 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6241 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6301 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6361 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6421 t
//
