LOCUS       Exported                6441 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    40_pAAV-ProC9-CatCh-GFP-WPRE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6441)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6441
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        682..878
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      729..864
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     791..810
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     regulatory      885..894
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             891..1814
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGA"
     CDS             1821..2537
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1866..1887)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2127..2146)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2474..2495
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2568..3156
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2621..2641)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3039..3050)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3188..3664
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3387..3406)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3704..3844
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3704..3833
                     /label=AAV2 ITR
     rep_origin      3919..4374
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4006..4025)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4216..4237
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4391..4410)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4510..4532
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4570..4588)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4656..4760
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4761..5621
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4979..4998)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5792..6380
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6281..6300
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtatgtag gggtctatgt gatttgcata tagttggggc
      181 ctaactgatt tgcatatgtt ttcggaagcg gtgatttgca taaacgtttt ccggacttga
      241 tttgcatatg taagaccgta gagtgatttg catatcgtag tccgaacatt gatttgcata
      301 tgatgacgca ggcactgatt tgcatacgaa agaacgaatg gtgatttgca taagtgggaa
      361 atgtatatga tttgcatatt cagcagaagg tactgatttg catacgcccc gcttaccggt
      421 gatttgcata tcatcggcac gtcattgatt tgcatagaga aagcactaca ctgatttgca
      481 taccgtctac ctggcgttga tttgcatata cgatgtaaaa aaatgatttg catagcattg
      541 ttgtgggatt gatttgcata agggcagcca tcgtatgatt tgcatatatg tggtatgcgt
      601 ttgatttgca tatttgttgc cagagtttga tttgcatatc tatagtggcc cgttgatttg
      661 catagctcga gatctgcgat ctgcatctca attagtcagc aaccatagtc ccgcccctaa
      721 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catcgctgac
      781 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
      841 agtgaggagg cttttttgga ggcctaggct tttgcaaagg atccgccacc atggattatg
      901 gaggcgccct gagtgccgtt gggcgcgagc tgctatttgt aacgaaccca gtagtcgtca
      961 atggctctgt acttgtgcct gaggaccagt gttactgcgc gggctggatt gagtcgcgtg
     1021 gcacaaacgg tgcccaaacg gcgtcgaacg tgctgcaatg gcttgctgct ggcttctcca
     1081 tcctactgct tatgttttac gcctaccaaa catggaagtc aacctgcggc tgggaggaga
     1141 tctatgtgtg cgctatcgag atggtcaagg tgattctcga gttcttcttc gagtttaaga
     1201 acccgtccat gctgtatcta gccacaggcc accgcgtcca gtggttgcgt tacgccgagt
     1261 ggcttctcac ctgcccggtc atttgcattc acctgtcaaa cctgacgggc ttgtccaacg
     1321 actacagcag gcgcaccatg ggtctgcttg tgtctgatat tggcacaatt gtgtggggcg
     1381 ccacttccgc catggccacc ggatacgtca aggtcatctt cttctgcctg ggtctgtgtt
     1441 atggtgctaa cacgttcttt cacgctgcca aggcctacat cgagggttac cacaccgtgc
     1501 cgaagggccg gtgtcgccag gtggtgactg gcatggcttg gctcttcttc gtatcatggg
     1561 gtatgttccc catcctgttc atcctcggcc ccgagggctt cggcgtcctg agcgtgtacg
     1621 gctccaccgt cggccacacc atcattgacc tgatgtcgaa gaactgctgg ggtctgctcg
     1681 gccactacct gcgcgtgctg atccacgagc atatcctcat ccacggcgac attcgcaaga
     1741 ccaccaaatt gaacattggt ggcactgaga ttgaggtcga gacgctggtg gaggacgagg
     1801 ccgaggctgg cgcggtaccc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     1861 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     1921 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     1981 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     2041 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     2101 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     2161 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     2221 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     2281 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     2341 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     2401 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     2461 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     2521 tggacgagct gtacaagtga taagaattcg atatcaagct tatcgataat caacctctgg
     2581 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     2641 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     2701 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     2761 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     2821 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     2881 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     2941 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tatgttgcca
     3001 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     3061 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     3121 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgagcg ctgctcgaga
     3181 gatctacggg tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc
     3241 cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta
     3301 ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg
     3361 gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca
     3421 atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc
     3481 cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta
     3541 gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta
     3601 cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt
     3661 ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg
     3721 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
     3781 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg
     3841 caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     3901 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     3961 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     4021 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     4081 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
     4141 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     4201 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     4261 gctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     4321 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt
     4381 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     4441 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     4501 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     4561 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     4621 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     4681 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     4741 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     4801 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     4861 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     4921 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     4981 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     5041 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     5101 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     5161 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     5221 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     5281 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     5341 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     5401 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     5461 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     5521 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     5581 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     5641 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     5701 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     5761 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     5821 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5881 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     5941 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6001 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6061 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6121 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6181 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6241 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6301 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6361 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6421 gctggccttt tgctcacatg t
//
