LOCUS       Exported                6501 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    42_pAAV-ProC41-CatCh-GFP-WPRE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6501)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6501
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        742..938
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      789..924
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     851..870
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     regulatory      945..954
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             951..1874
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGA"
     CDS             1881..2597
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1926..1947)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2187..2206)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2534..2555
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2628..3216
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2681..2701)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3099..3110)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3248..3724
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3447..3466)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3764..3904
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3764..3893
                     /label=AAV2 ITR
     rep_origin      3979..4434
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4066..4085)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4276..4297
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4451..4470)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4570..4592
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4630..4648)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4716..4820
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4821..5681
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5039..5058)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5852..6440
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6341..6360
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttagact ggagtgccaa tgcataaatt cattgctagc
      181 tattaaatat gcataaattc atctccgtgg ccaatacatg cataaattca tgcggggtcg
      241 cccttaatgc ataaattcat tgtgttaaat atagtatgca taaattcata gcgtcggaac
      301 cggtatgcat aaattcattt taccgacttt tgaatgcata aattcattta gacacacaaa
      361 atatgcataa attcattcga ccccgcagct catgcataaa ttcatccgta cggttcctgc
      421 atgcataaat tcattgtttt tatggaaata tgcataaatt catgtcaagc acgtaaccat
      481 gcataaattc atacttgatg gttgggcatg cataaattca tggtgttaaa tgttatatgc
      541 ataaattcat cctccaaatc actacatgca taaattcatg aaagacgtcg ggtaatgcat
      601 aaattcatcc atttatcgta accatgcata aattcatgag acgattgggc ctatgcataa
      661 attcataact gaagaattcc gatgcataaa ttcatcagga acacgatacg atgcataaat
      721 tcatgctcga gatctgcgat ctgcatctca attagtcagc aaccatagtc ccgcccctaa
      781 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catcgctgac
      841 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
      901 agtgaggagg cttttttgga ggcctaggct tttgcaaagg atccgccacc atggattatg
      961 gaggcgccct gagtgccgtt gggcgcgagc tgctatttgt aacgaaccca gtagtcgtca
     1021 atggctctgt acttgtgcct gaggaccagt gttactgcgc gggctggatt gagtcgcgtg
     1081 gcacaaacgg tgcccaaacg gcgtcgaacg tgctgcaatg gcttgctgct ggcttctcca
     1141 tcctactgct tatgttttac gcctaccaaa catggaagtc aacctgcggc tgggaggaga
     1201 tctatgtgtg cgctatcgag atggtcaagg tgattctcga gttcttcttc gagtttaaga
     1261 acccgtccat gctgtatcta gccacaggcc accgcgtcca gtggttgcgt tacgccgagt
     1321 ggcttctcac ctgcccggtc atttgcattc acctgtcaaa cctgacgggc ttgtccaacg
     1381 actacagcag gcgcaccatg ggtctgcttg tgtctgatat tggcacaatt gtgtggggcg
     1441 ccacttccgc catggccacc ggatacgtca aggtcatctt cttctgcctg ggtctgtgtt
     1501 atggtgctaa cacgttcttt cacgctgcca aggcctacat cgagggttac cacaccgtgc
     1561 cgaagggccg gtgtcgccag gtggtgactg gcatggcttg gctcttcttc gtatcatggg
     1621 gtatgttccc catcctgttc atcctcggcc ccgagggctt cggcgtcctg agcgtgtacg
     1681 gctccaccgt cggccacacc atcattgacc tgatgtcgaa gaactgctgg ggtctgctcg
     1741 gccactacct gcgcgtgctg atccacgagc atatcctcat ccacggcgac attcgcaaga
     1801 ccaccaaatt gaacattggt ggcactgaga ttgaggtcga gacgctggtg gaggacgagg
     1861 ccgaggctgg cgcggtaccc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     1921 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     1981 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     2041 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     2101 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     2161 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     2221 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     2281 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     2341 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     2401 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     2461 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     2521 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     2581 tggacgagct gtacaagtga taagaattcg atatcaagct tatcgataat caacctctgg
     2641 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     2701 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     2761 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     2821 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     2881 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     2941 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     3001 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tatgttgcca
     3061 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     3121 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     3181 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgagcg ctgctcgaga
     3241 gatctacggg tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc
     3301 cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta
     3361 ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg
     3421 gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca
     3481 atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc
     3541 cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta
     3601 gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta
     3661 cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt
     3721 ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg
     3781 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
     3841 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg
     3901 caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     3961 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     4021 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     4081 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     4141 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
     4201 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     4261 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     4321 gctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     4381 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt
     4441 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     4501 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     4561 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     4621 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     4681 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     4741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     4801 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     4861 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     4921 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     4981 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     5041 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     5101 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     5161 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     5221 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     5281 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     5341 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     5401 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     5461 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     5521 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     5581 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     5641 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     5701 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     5761 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     5821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     5881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     6001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6481 gctggccttt tgctcacatg t
//
