LOCUS Exported 6461 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina. ACCESSION . VERSION . KEYWORDS 45_pAAV-ProC10-CatCh-GFP-WPRE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6461) AUTHORS Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B TITLE Targeting neuronal and glial cell types with synthetic promoter AAVs in mice, non-human primates and humans. JOURNAL Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 10.1038/s41593-019-0431-2. PUBMED 31285614 REFERENCE 2 (bases 1 to 6461) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6461 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..130 /label=AAV2 ITR (alternate) /note="Functional equivalent of wild-type AAV2 ITR" promoter 702..898 /label=SV40 promoter /note="SV40 early promoter" rep_origin 749..884 /label=SV40 ori /note="SV40 origin of replication" primer_bind 811..830 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" regulatory 905..914 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 911..1834 /codon_start=1 /product="N-terminal fragment of channelrhodopsin-2 from the alga Chlamydomonas reinhardtii (Nagel et al., 2003)" /label=ChR2 /note="blue light-activated cation channel" /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI RKTTKLNIGGTEIEVETLVEDEAEAGA" CDS 1841..2557 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(1886..1907) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(2147..2166) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 2494..2515 /label=EGFP-C /note="EGFP, forward primer" misc_feature 2588..3176 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2641..2661) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(3059..3070) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 3208..3684 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(3407..3426) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" repeat_region 3724..3864 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 3724..3853 /label=AAV2 ITR rep_origin 3939..4394 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4026..4045) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4236..4257 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(4411..4430) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 4530..4552 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(4590..4608) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 4676..4780 /gene="bla" /label=AmpR promoter CDS 4781..5641 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4999..5018) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5812..6400 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6301..6320 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggccgcac gcgttgggtc tgtcagaaca taaatcaata atttgccgat 181 tttagtataa atcaataact accattagag tccataaatc aataaacacg tctcattttg 241 ataaatcaat aaataggagt caccgttata aatcaataat ataggctcgg agatataaat 301 caataagggg gtggtgaaat aataaatcaa taaacaacgg cttacataat aaatcaataa 361 tcgcgtcacg tcttgataaa tcaataacat ggtctgttct gtataaatca ataaaaccct 421 ctaccaagaa taaatcaata atggcccgat ctatatataa atcaataagg gacacgggcc 481 cgaataaatc aataataaaa tacgctagga ataaatcaat aaaccataat acaatcaata 541 aatcaataac cgcccctaca cgacataaat caataatgcc actagggacc tataaatcaa 601 taactagcaa tgcttggcat aaatcaataa tttctcacga agataataaa tcaataactg 661 agttgcgata gcataaatca ataagctcga gatctgcgat ctgcatctca attagtcagc 721 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 781 ttctccgccc catcgctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 841 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaagg 901 atccgccacc atggattatg gaggcgccct gagtgccgtt gggcgcgagc tgctatttgt 961 aacgaaccca gtagtcgtca atggctctgt acttgtgcct gaggaccagt gttactgcgc 1021 gggctggatt gagtcgcgtg gcacaaacgg tgcccaaacg gcgtcgaacg tgctgcaatg 1081 gcttgctgct ggcttctcca tcctactgct tatgttttac gcctaccaaa catggaagtc 1141 aacctgcggc tgggaggaga tctatgtgtg cgctatcgag atggtcaagg tgattctcga 1201 gttcttcttc gagtttaaga acccgtccat gctgtatcta gccacaggcc accgcgtcca 1261 gtggttgcgt tacgccgagt ggcttctcac ctgcccggtc atttgcattc acctgtcaaa 1321 cctgacgggc ttgtccaacg actacagcag gcgcaccatg ggtctgcttg tgtctgatat 1381 tggcacaatt gtgtggggcg ccacttccgc catggccacc ggatacgtca aggtcatctt 1441 cttctgcctg ggtctgtgtt atggtgctaa cacgttcttt cacgctgcca aggcctacat 1501 cgagggttac cacaccgtgc cgaagggccg gtgtcgccag gtggtgactg gcatggcttg 1561 gctcttcttc gtatcatggg gtatgttccc catcctgttc atcctcggcc ccgagggctt 1621 cggcgtcctg agcgtgtacg gctccaccgt cggccacacc atcattgacc tgatgtcgaa 1681 gaactgctgg ggtctgctcg gccactacct gcgcgtgctg atccacgagc atatcctcat 1741 ccacggcgac attcgcaaga ccaccaaatt gaacattggt ggcactgaga ttgaggtcga 1801 gacgctggtg gaggacgagg ccgaggctgg cgcggtaccc atggtgagca agggcgagga 1861 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa 1921 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt 1981 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta 2041 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc 2101 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta 2161 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa 2221 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa 2281 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa 2341 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac 2401 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc 2461 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc 2521 cgccgggatc actctcggca tggacgagct gtacaagtga taagaattcg atatcaagct 2581 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta 2641 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc 2701 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga 2761 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac 2821 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc 2881 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc 2941 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg 3001 gctgctcgcc tatgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc 3061 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc 3121 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg 3181 ataccgagcg ctgctcgaga gatctacggg tggcatccct gtgacccctc cccagtgcct 3241 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt 3301 tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta 3361 tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca 3421 agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg 3481 attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc 3541 taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac 3601 tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga 3661 accactgctc ccttccctgt ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc 3721 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg 3781 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg 3841 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt 3901 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt 3961 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 4021 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 4081 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc 4141 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt 4201 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac 4261 aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc 4321 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt 4381 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag 4441 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc 4501 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc 4561 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa 4621 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg 4681 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata 4741 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg 4801 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac 4861 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact 4921 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat 4981 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga 5041 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac 5101 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat 5161 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac 5221 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct 5281 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac 5341 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga 5401 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg 5461 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact 5521 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac 5581 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta 5641 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt 5701 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga 5761 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc 5821 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt 5881 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc 5941 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc 6001 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg 6061 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg 6121 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga 6181 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc 6241 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg 6301 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg 6361 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt 6421 tttacggttc ctggcctttt gctggccttt tgctcacatg t //