LOCUS Exported 3347 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION BglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae. ACCESSION . VERSION . KEYWORDS pPEPX-Plac SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3347) AUTHORS Keller LE, Rueff AS, Kurushima J, Veening JW TITLE Three New Integration Vectors and Fluorescent Proteins for Use in the Opportunistic Human Pathogen Streptococcus pneumoniae. JOURNAL Genes (Basel). 2019 May 22;10(5). pii: genes10050394. doi: 10.3390/genes10050394. PUBMED 31121970 REFERENCE 2 (bases 1 to 3347) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3347 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 203..274 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 290..317 /label=T7Te terminator /note="phage T7 early transcription terminator" protein_bind 414..433 /label=lac operator (symmetric) /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG). The symmetric lac operator was optimized for tight binding of lac repressor." rep_origin 1120..1708 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1609..1628 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 gcatgcggca aagcactcaa aagtttacct tatgggtgct tttttcgtgc ttttttgaaa 61 agacaaaaaa aagaaccttg ccaagcaaga ttcttttgat agggtggtct ccgatcgtgt 121 actgcagaaa actagtcgca aaaaaccccg cttcggcggg gttttttcgc tctagacggt 181 gatcaacacg ctagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 241 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc 301 gggtgggcct ttctgcgttt atagaattca ctagtaattg tgaggcggat aacaattctc 361 actcattcta cagtttattc ttgacattgc actgtccccc tggtataata actaattgtg 421 agcgctcaca attaagatct aaataaataa ggaggaaaat taatgccgga tccctccagt 481 aactcgagaa aaaaaaaccg cgcccctgac agggcgcggt ttttttttaa gtggacacct 541 acgtatggta actgtacagg tcgctgaaac ttctatcgtc aagaagaatc accaaatccc 601 tcgtatcatc aaccaaaaga ttgctcaaaa gttaattgaa aagatttcta tgactgatat 661 tgcccatcag ctttccatct caacttcaac tgttattcgt aagctcaatg attttcactt 721 taaacatgat ttttcgcgtc ttcctgagat tatgtcttgg gatgagtatg cttttacaaa 781 gggaaagatg agcttcattg cgcaagattt taacaatctc aatatcatca ctgttcttga 841 gggaagaaca caagctatca ttcgaaatca ctttcttcgc tacgatagag ttgtccgatg 901 tcgcgtcaaa attattacta tggatatgtt tagcccttac tatgacttgg ctaaacagct 961 tcgctttcaa atttctaggc tcaggctgaa acagtctccc agactgtttc actcccgaat 1021 gctaaaatcg ttcttgatcg ctttcacatt ggacgtcctg accatggagt caagtgtaag 1081 gcactggtac caccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1141 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1201 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1261 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1321 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1381 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1441 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1501 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1561 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 1621 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 1681 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 1741 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccgcg gccgcagtta 1801 aagaacttgg actgcgctct aaaaccaacg tggtcagtct ggacaagatt attattgata 1861 aaaaatcctc actgataacc ggtttcccag agtttcggat ggtttggaag gtggtccgcc 1921 agcgtgtggt ggatgatctg gtatccgttc tggatacgga ctatctggat atggaactaa 1981 tcccaaatct cactcgccaa atcgctgagc agtctatcta ttcttatata gaaaatggcc 2041 tcaaactcct tattgattat gctcagaagg aaatcaccat tgaccactca agcgaccgag 2101 acaagattct catggacatt ggcaaagacc cttatgtcgt ttcgattaaa tcaaaagtct 2161 atctccaaga cggacgccaa tttcagttta ccgaaagtcg ccataagtta gaaaaattta 2221 gatttgtaga ttttgcaaaa cgcaagaaat aaaagactga gacaccagat catggctaga 2281 aagcttctag caaaaaactg gacggagtcc cgtccagaaa agtaagcact tgttattgca 2341 ataaaattag cctaattgag agaagtttct atagaatttt tcatatactt aacgagtgct 2401 ttcacctttg aatatagtcc ttcccactta tcatcacact ctccccgata gccttttcta 2461 gctatatcca gtaaagttac atgctcttta ggtaaaagag gtatagccca ttcagcagcg 2521 acatctttcg aggtaatttc accagtagtc actgtttgcc acattcgagc tagggttaaa 2581 attacattac gctcatcacc ttttatcccc tcaattagtt ctggcaaaga atccttaatt 2641 gctcttcgaa tatctgtcaa aggtacggag acaagtatac ttgaagaatc aggaccaaat 2701 agagaaatac tattctttct tgcttgtgct aaaacaatag ccaaatcagg atcatagctt 2761 ggttcctgaa tttgtccatt ctcaaattca cccctgagcc actcaccgta tataaattct 2821 ctttttggag gatattgcca agggacaact tcactcctat ttataaccgt aacttcaagt 2881 ggtctaacag aatccgtatt tccaatcctt cctgatatag tcattagtct ttctgttagt 2941 ttttttcgag ttaattgagg taaactatga ttcacgacga ctagaacatc tacatcgctg 3001 ttaatgcgta aaccaccatt tactgctgaa ccaaatagat atactccaac tattgaactt 3061 ccaaataaat cttttacgat ttttaatgtt tgaatcgctt gatttggtat ttttccgtta 3121 atcaaattgc tcatgatttc acctcgttga ttatgttcat ataaagttta tattgatact 3181 caatttactt accctagatt ggacatatac ttaaattact gttcaataaa gctgaccgtt 3241 agcgtttaag tacatccttt cacaatttgt ctacagaaca gtactggcct acctagtata 3301 gcattttgtg aagttttttt ctagtccaag ctcacaaaaa tccaaag //