LOCUS       Exported               14177 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  gateway cloning destination vector for expressing enhancer driven 
            CD4-tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    pDEST-HemmarR2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14177)
  AUTHORS   Poe AR, Wang B, Sapar ML, Ji H, Li K, Onabajo T, Fazliyeva R, Gibbs 
            M, Qiu Y, Hu Y, Han C
  TITLE     Robust CRISPR/Cas9-Mediated Tissue Specific Mutagenesis Reveals Gene
            Redundancy and Perdurance in Drosophila.
  JOURNAL   Genetics. 2018 Nov 30. pii: genetics.118.301736. doi: 
            10.1534/genetics.118.301736.
  PUBMED    30504366
REFERENCE   2  (bases 1 to 14177)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14177
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1..306
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     185..204
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(347..471)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1607..3034
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     protein_bind    3747..4132
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, and
                     Mod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     primer_bind     complement(4142..4160)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(4612..4683,5085..5358,5433..6087,6149..6464,6668..6799,
                     6870..7484)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    8263..8848
                     /label=P element 5' end
     rep_origin      complement(9083..9671)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9163..9182)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9842..10702)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10465..10484
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10703..10807)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10875..10893
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10931..10953)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11053..11072
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    11281..11350
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    11894..12126
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    12524..12909
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, and
                     Mod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    12944..13068
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        13093..13123
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             13177..13836
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(13243..13262)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta
       61 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt
      121 ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc
      181 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg
      241 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
      301 atataaatgt caggctccct tatacacagc cagtctgcag gtcgaccata gtgactggat
      361 atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt
      421 gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg tgatttgtca
      481 aggtaccgag cgccggagta taaatagagg cgcttcgtct acggagctca gttgtgaatg
      541 aatgttcgag ccgagcagac gtgccgcgcg aaagctaagc aaataaacaa gcgcagctga
      601 gcaagctaaa caatcgtaaa gtgcaagtta aagtgaatca attaaaagta accagcaacc
      661 aagtaaatca actgcaacta ctgaaatctg ccggtgagat tgtacaccct tcttaaagat
      721 ccccttcaaa gtctaaattc ttccttttcg tttagccact cgagagaaaa ccggtacgcg
      781 tgaattcaac ttaaaaaaaa aaatcaaaat gaatcccaag agcgaagtcc tcattgcagc
      841 cgtgctgttc atgctgctgg cctgcgtcca gtgtcaagct agcggtggcg gcggaagtgg
      901 aggtggaggc tcgttccaga aggcctccag catagtctat aagaaagagg gggaacaggt
      961 ggagttctcc ttcccactcg cctttacagt tgaaaagctg acgggcagtg gcgagctgtg
     1021 gtggcaggcg gagagggctt cctcctccaa gtcttggatc acctttgacc tgaagaacaa
     1081 ggaagtgtct gtaaaacggg ttacccagga ccctaagctc cagatgggca agaagctccc
     1141 gctccacctc accctgcccc aggccttgcc tcagtatgct ggctctggaa acctcaccct
     1201 ggcccttgaa gcgaaaacag gaaagttgca tcaggaagtg aacctggtgg tgatgagagc
     1261 cactcagctc cagaaaaatt tgacctgtga ggtgtgggga cccacctccc ctaagctgat
     1321 gctgagcttg aaactggaga acaaggaggc aaaggtctcg aagcgggaga aggcggtgtg
     1381 ggtgctgaac cctgaggcgg ggatgtggca gtgtctgctg agtgactcgg gacaggtcct
     1441 gctggaatcc aacatcaagg ttctgcccac atggtccacc ccggtgcagc caatggccct
     1501 gattgtgctg gggggcgtcg ccggcctcct gcttttcatt gggctaggca tcttcttctg
     1561 tgtcaggtgc cggcaccgaa ggcgcggcgg aagcggaggc ggatccatgg tgagcaaggg
     1621 cgaggaggtc atcaaagagt tcatgcgctt caaggtgcgc atggagggct ccatgaacgg
     1681 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     1741 caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca
     1801 gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg attacaagaa
     1861 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggtct
     1921 ggtgaccgtg acccaggact cctccctgca ggacggcacg ctgatctaca aggtgaagat
     1981 gcgcggcacc aacttccccc ccgacggccc cgtaatgcag aagaagacca tgggctggga
     2041 ggcctccacc gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccaccaggc
     2101 cctgaagctg aaggacggcg gccactacct ggtggagttc aagaccatct acatggccaa
     2161 gaagcccgtg caactgcccg gctactacta cgtggacacc aagctggaca tcacctccca
     2221 caacgaggac tacaccatcg tggaacagta cgagcgctcc gagggccgcc accacctgtt
     2281 cctggggcat ggcaccggca gcaccggcag cggcagctcc ggcaccgcct cctccgagga
     2341 caacaacatg gccgtcatca aagagttcat gcgcttcaag gtgcgcatgg agggctccat
     2401 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     2461 gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc
     2521 cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca tccccgatta
     2581 caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     2641 cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga tctacaaggt
     2701 gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg
     2761 ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca
     2821 ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga ccatctacat
     2881 ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc tggacatcac
     2941 ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg gccgccacca
     3001 cctgttcctg tacggcatgg acgagctgta caaggagaac gcaaacagct tttgctatga
     3061 aaatgaagtt gccctctaat ctagaattaa ttaactaagc cagtcggcaa tcggacgcct
     3121 tcgaaacatg caacactaat gtttaattca gatttcagca gagacaagct aaaacaccga
     3181 cgagttgtaa tcatttctgt gcgccagcat atatttctta tatacaacgt aatacataat
     3241 tatgtaattc tagcatctcc ccaacactca catacaaaca aacaaaaaat acaaacacac
     3301 aaaacgtatt tacccgcacg catccttggc gaggttgagt atgaaacaaa aacaaaactt
     3361 aatttagagc aaagtaatta cacgaataaa tttaataaaa aaaaactata ataaaaacgc
     3421 caatcatgtt atttccaaaa aaaaaaaaaa aaaaaaaact agacgagatg tttgcgcttg
     3481 tgtcgatgaa tgtaaagaat tgcaactaca tagatggtaa ttaatttcag ctgtaagaag
     3541 tacaagtttt tcataattgt gagaaaagtg cagtagtatc gcttaaagtt cagttttaat
     3601 cagggcattt agcagctcga acccaatgta tttgtaagcc caataaacgt gtaaatgcct
     3661 agagcaaaat atcacaactg tagcttttca tccgaaagat aataatgaat agactgacct
     3721 gacgtttttc aaaatgcgta actagtcacg taataagtgt gcgttgaatt tattcgcaaa
     3781 aacattgcat attttcggca aagtaaaatt ttgttgcata ccttatcaaa aaataagtgc
     3841 tgcatacttt ttagagaaac caaataattt tttattgcat acccgttttt aataaaatac
     3901 attgcatacc ctcttttaat aaaaaatatt gcatactttg acgaaacaaa ttttcgttgc
     3961 atacccaata aaagattatt atattgcata cccgttttta ataaaataca ttgcataccc
     4021 tcttttaata aaaaatattg catacgttga cgaaacaaat tttcgttgca tacccaataa
     4081 aagattatta tattgcatac cttttcttgc cataccattt agccgatcaa ttctagtatg
     4141 tatgtaagtt aataaaaccc atttttgcgg aaagtagata aaaaaaacat tttttttttt
     4201 tactgcactg gatatcattg aacttatctg atcagtttta aatttacttc gatccaaggg
     4261 tatttgatgt accaggttct ttcgattacc tctcactcaa aatgacattc cactcaaagt
     4321 cagcgctgtt tgcctccttc tctgtccaca gaaatatcgc cgtctctttc gccgctgcgt
     4381 ccgctatctc tttcgccacc gtttgtagcg ttacgtagcg tcaatgtccg ccttcagttg
     4441 cattttgtca gcggtttcgt gacgaagctc caagcggttt acgccatcaa ttaaacacaa
     4501 agtgctgtgc caaaactcct ctcgcttctt atttttgttt gttttttgag tgattggggt
     4561 ggtgattggt tttgggtggg taagcagggg aaagtgtgaa aaatcccggc aatgggccaa
     4621 gaggatcagg agctattaat tcgcggaggc agcaaacacc catctgccga gcatctgaac
     4681 aatgtgagta gtacatgtgc atacatctta agttcacttg atctatagga actgcgattg
     4741 caacatcaaa ttgtctgcgg cgtgagaact gcgacccaca aaaatcccaa accgcaattg
     4801 cacaaacaaa tagtgacacg aaacagatta ttctggtagc tgttctcgct atataagaca
     4861 atttttgaga tcatatcatg atcaagacat ctaaaggcat tcattttcga ctatattctt
     4921 ttttacaaaa aatataacaa ccagatattt taagctgatc ctagatgcac aaaaaataaa
     4981 taaaagtata aacctacttc gtaggatact tcggggtact ttttgttcgg ggttagatga
     5041 gcataacgct tgtagttgat atttgagatc ccctatcatt gcagggtgac agcggagcgg
     5101 cttcgcagag ctgcattaac cagggcttcg ggcaggccaa aaactacggc acgctccggc
     5161 cacccagtcc gccggaggac tccggttcag ggagcggcca actagccgag aacctcacct
     5221 atgcctggca caatatggac atctttgggg cggtcaatca gccgggctcc ggatggcggc
     5281 agctggtcaa ccggacacgc ggactattct gcaacgagcg acacataccg gcgcccagga
     5341 aacatttgct caagaacggt gagtttctat tcgcagtcgg ctgatctgtg tgaaatctta
     5401 ataaagggtc caattaccaa tttgaaactc agtttgcggc gtggcctatc cgggcgaact
     5461 tttggccgtg atgggcagtt ccggtgccgg aaagacgacc ctgctgaatg cccttgcctt
     5521 tcgatcgccg cagggcatcc aagtatcgcc atccgggatg cgactgctca atggccaacc
     5581 tgtggacgcc aaggagatgc aggccaggtg cgcctatgtc cagcaggatg acctctttat
     5641 cggctcccta acggccaggg aacacctgat tttccaagcc atggtgcgga tgccacgaca
     5701 tctgacctat cggcagcgag tggcccgcgt ggatcaggtg atccaggagc tttcgctcag
     5761 caaatgtcag cacacgatca tcggtgtgcc cggcagggtg aaaggtctgt ccggcggaga
     5821 aaggaagcgt ctggcattcg cctccgaggc tctaaccgat ccgccgcttc tgatctgcga
     5881 tgagcccacc tccggactgg actcctttac cgcccacagc gtcgtccagg tgctgaagaa
     5941 gctgtcgcag aagggcaaga ccgtcatcct gaccattcat cagccgtctt ccgagctgtt
     6001 tgagctcttt gacaagatcc ttctgatggc cgagggcagg gtagctttct tgggcactcc
     6061 cagcgaagcc gtcgacttct tttcctagtg agttcgatgt gtttattaag ggtatctagt
     6121 attacataac atctcaactc ctatccagcg tgggtgccca gtgtcctacc aactacaatc
     6181 cggcggactt ttacgtacag gtgttggccg ttgtgcccgg acgggagatc gagtcccgtg
     6241 atcggatcgc caagatatgc gacaattttg ccattagcaa agtagcccgg gatatggagc
     6301 agttgttggc caccaaaaat ctggagaagc cactggagca gccggagaat gggtacacct
     6361 acaaggccac ctggttcatg cagttccggg cggtcctgtg gcgatcctgg ctgtcggtgc
     6421 tcaaggaacc actcctcgta aaagtgcgac ttattcagac aacggtgagt ggttccagtg
     6481 gaaacaaatg atataacgct tacaattctt ggaaacaaat tcgctagatt ttagatagaa
     6541 ttgcctgatt ccacaccctt cttagttttt ttcaatgaga tgtatagttt atagttttgc
     6601 agaagataaa taaatttcat ttaactcgcg aatattaatg agatgcgagt aacattttaa
     6661 tttgcagatg gttgccatct tgattggcct catctttttg ggccaacaac tcacgcaagt
     6721 gggtgtgatg aatatcaacg gagccatctt cctcttcctg accaacatga cctttcaaaa
     6781 cgtctttgcc acgataaatg taagtcatgt ttagaataca tttgcatttc aataatttac
     6841 taactttcta atgaatcgat tcgatttagg tgttcacctc agagctgcca gtttttatga
     6901 gggaggcccg aagtcgactt tatcgctgtg acacatactt tctgggcaaa acgattgccg
     6961 aattgccgct ttttctcaca gtgccactgg tcttcacggc gattgcctat ccgatgatcg
     7021 gactgcgggc cggagtgctg cacttcttca actgcctggc gctggtcact ctggtggcca
     7081 atgtgtcaac gtccttcgga tatctaatat cctgcgccag ctcctcgacc tcgatggcgc
     7141 tgtctgtggg tccgccggtt atcataccat tcctgctctt tggcggcttc ttcttgaact
     7201 cgggctcggt gccagtatac ctcaaatggt tgtcgtacct ctcatggttc cgttacgcca
     7261 acgagggtct gctgattaac caatgggcgg acgtggagcc gggcgaaatt agctgcacat
     7321 cgtcgaacac cacgtgcccc agttcgggca aggtcatcct ggagacgctt aacttctccg
     7381 ccgccgatct gccgctggac tacgtgggtc tggccattct catcgtgagc ttccgggtgc
     7441 tcgcatatct ggctctaaga cttcgggccc gacgcaagga gtagccgaca tatatccgaa
     7501 ataactgctt gttttttttt tttaccatta ttaccatcgt gtttactgtt tattgccccc
     7561 tcaaaaagct aatgtaatta tatttgtgcc aataaaaaca agatatgacc tatagaatac
     7621 aagtatttcc ccttcgaaca tccccacaag tagactttgg atttgtcttc taaccaaaag
     7681 acttacacac ctgcatacct tacatcaaaa actcgtttat cgctacataa aacaccggga
     7741 tatatttttt atatacatac ttttcaaatc gcgcgccctc ttcataattc acctccacca
     7801 caccacgttt cgtagttgct ctttcgctgt ctcccacccg ctctccgcaa cacattcacc
     7861 ttttgttcga cgaccttgga gcgactgtcg ttagttccgc gcgattcggt tcgctcaaat
     7921 ggttccgagt ggttcatttc gtctcaatag aaattagtaa taaatatttg tatgtacaat
     7981 ttatttgctc caatatattt gtatatattt ccctcacagc tatatttatt ctaatttaat
     8041 attatgactt tttaaggtaa ttttttgtga cctgttcgga gtgattagcg ttacaatttg
     8101 aactgaaagt gacatccagt gtttgttcct tgtgtagatg catctcaaaa aaatggtggg
     8161 cataatagtg ttgtttatat atatcaaaaa taacaactat aataataaga atacatttaa
     8221 tttagaaaat gcttggattt cactggaact agaattaatt cggctgctgc tctaaacgac
     8281 gcatttcgta ctccaaagta cgaatttttt ccctcaagct cttattttca ttaaacaatg
     8341 aacaggacct aacgcacagt cacgttattg tttacataaa tgattttttt tactattcaa
     8401 acttactctg tttgtgtact cccactggta tagccttctt ttatcttttc tggttcaggc
     8461 tctatcactt tactaggtac ggcatctgcg ttgagtcgcc tccttttaaa tgtctgacct
     8521 tttgcaggtg cagccttcca ctgcgaatca ttaaagtggg tatcacaaat ttgggagttt
     8581 tcaccaaggc tgcacccaag gctctgctcc cacaattttc tcttaatagc acacttcggc
     8641 acgtgaatta attttactcc agtcacagct ttgcagcaaa atttgcaata tttcattttt
     8701 ttttattcca cgtaagggtt aatgttttca aaaaaaaatt cgtccgcaca caacctttcc
     8761 tctcaacaag caaacgtgca ctgaatttaa gtgtatactt cggtaagctt cggctatcga
     8821 cgggaccacc ttatgttatt tcatcatggg ccagacccac gtagtccagc ggcagatcgg
     8881 cggcggagaa gttaagcgtc tccaggatga ccttgcccga actggggcac gtggtgttcg
     8941 acgatgtgca gctaatttcg cccggctcca cgtccgccca ttggttaatc agcagaccct
     9001 cgttggcgta acggaaccat gagaggtacg acaaccattt gaggtatact ggcaccgagc
     9061 ccgagttcaa gaagaaggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     9121 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     9181 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     9241 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     9301 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     9361 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     9421 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     9481 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     9541 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     9601 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     9661 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     9721 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     9781 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     9841 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     9901 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     9961 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
    10021 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
    10081 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
    10141 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
    10201 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
    10261 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    10321 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    10381 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    10441 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    10501 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    10561 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    10621 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    10681 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    10741 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    10801 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
    10861 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
    10921 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
    10981 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
    11041 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccattcg acatgcccgc
    11101 cgtgaccgtc gagaacccgc tgacgctgcc ccgcgtatcc gcacccgccg acgccgtcgc
    11161 acgtcccgtg ctcaccgtga ccaccgcgcc cagcggtttc gagggcgagg gcttcccggt
    11221 gcgccgcgcg ttcgccggga tcaactaccg ccacctcgac ccgttcatca tgatggacca
    11281 gatgggtgag gtggagtacg cgcccgggga gcccaagggc acgccctggc acccgcaccg
    11341 cggcttcgag accgtgacct acatcgtcga tatgcggtgt gaaataccgc accgaatcgc
    11401 gcggaactaa cgacagtcgc tccaaggtcg tcgaacaaaa ggtgaatgtg ttgcggagag
    11461 cgggtgggag acagcgaaag agcaactacg aaacgtggtg tggtggaggt gaattatgaa
    11521 gagggcgcgc gatttgaaaa gtatgtatat aaaaaatata tcccggtgtt ttatgtagcg
    11581 ataaacgagt ttttgatgta aggtatgcag gtgtgtaagt cttttggtta gaagacaaat
    11641 ccaaagtcta cttgtgggga tgttcgaagg ggaaatactt gtattctata ggtcatatct
    11701 tgtttttatt ggcacaaata taattacatt agctttttga gggggcaata aacagtaaac
    11761 acgatggtaa taatggtaaa aaaaaaaaac aagcagttat ttcggatata tgtcggctac
    11821 tccttgcgtc gggcccgaag tcttagagcc agatatgcga gcacccggaa gctcacgatg
    11881 agaatggcca gaccatgatg aaataacata aggtggtccc gtcggcaaga gacatccact
    11941 taacgtatgc ttgcaataag tgcgagtgaa aggaatagta ttctgagtgt cgtattgagt
    12001 ctgagtgaga cagcgatatg attgttgatt aacccttagc atgtccgtgg ggtttgaatt
    12061 aactcataat attaattaga cgaaattatt tttaaagttt tatttttaat aatttgcgag
    12121 tacgcaaagc ttcctaggtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg
    12181 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
    12241 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
    12301 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
    12361 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
    12421 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
    12481 aggggctcgc gccagccgaa ctgttcgcca agcttggctg catcacgtaa taagtgtgcg
    12541 ttgaatttat tcgcaaaaac attgcatatt ttcggcaaag taaaattttg ttgcatacct
    12601 tatcaaaaaa taagtgctgc atacttttta gagaaaccaa ataatttttt attgcatacc
    12661 cgtttttaat aaaatacatt gcataccctc ttttaataaa aaatattgca tactttgacg
    12721 aaacaaattt tcgttgcata cccaataaaa gattattata ttgcataccc gtttttaata
    12781 aaatacattg cataccctct tttaataaaa aatattgcat acgttgacga aacaaatttt
    12841 cgttgcatac ccaataaaag attattatat tgcatacctt ttcttgccat accatttagc
    12901 cgatcaattg tgctcggcaa cagcatgctg cagaagactt atcacaagtt tgtacaaaaa
    12961 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
    13021 aaaacagact acataatact gtaaaacaca acatatccag tcactatggc ggccgcatta
    13081 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag
    13141 gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
    13201 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
    13261 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
    13321 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
    13381 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
    13441 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
    13501 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
    13561 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
    13621 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
    13681 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
    13741 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta
    13801 caacagtact gcgatgagtg gcagggcggg gcgtaaacgc gtggatccgg cttactaaaa
    13861 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat
    13921 atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag
    13981 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt
    14041 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa
    14101 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga
    14161 gaacaggggc tggtgaa
//
