LOCUS Exported 6441 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina. ACCESSION . VERSION . KEYWORDS 108_pAAV-ProC18-CatCh-GFP-WPRE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6441) AUTHORS Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B TITLE Targeting neuronal and glial cell types with synthetic promoter AAVs in mice, non-human primates and humans. JOURNAL Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 10.1038/s41593-019-0431-2. PUBMED 31285614 REFERENCE 2 (bases 1 to 6441) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6441 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..130 /label=AAV2 ITR (alternate) /note="Functional equivalent of wild-type AAV2 ITR" promoter 682..878 /label=SV40 promoter /note="SV40 early promoter" rep_origin 729..864 /label=SV40 ori /note="SV40 origin of replication" primer_bind 791..810 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" regulatory 885..894 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 891..1814 /codon_start=1 /product="N-terminal fragment of channelrhodopsin-2 from the alga Chlamydomonas reinhardtii (Nagel et al., 2003)" /label=ChR2 /note="blue light-activated cation channel" /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI RKTTKLNIGGTEIEVETLVEDEAEAGA" CDS 1821..2537 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(1866..1887) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(2127..2146) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 2474..2495 /label=EGFP-C /note="EGFP, forward primer" misc_feature 2568..3156 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2621..2641) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(3039..3050) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 3188..3664 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(3387..3406) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" repeat_region 3704..3844 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 3704..3833 /label=AAV2 ITR rep_origin 3919..4374 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4006..4025) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4216..4237 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(4391..4410) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 4510..4532 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(4570..4588) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 4656..4760 /gene="bla" /label=AmpR promoter CDS 4761..5621 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4979..4998) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5792..6380 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6281..6300 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggccgcac gcgttggccg gctctcgatg gtaattatct aacaaaatcg 181 acgacggtaa ttatctctaa aagtgtgttt cggtaattat cttgatgctt accgaacggt 241 aattatctgg gatctacagt tgcggtaatt atctgtagag gagtggggcg gtaattatct 301 tcaaccgaac agcatggtaa ttatctcatt cttcggattt gggtaattat ctcggaattt 361 ataggtgggt aattatcttt agaatttgct cacggtaatt atctaatgat cagcaagccg 421 gtaattatct tgctcctcat gaggtggtaa ttatctagca ggatttacaa tggtaattat 481 ctgctgtgtg gacatctggt aattatctct gtcataagga cctggtaatt atcttgaccg 541 tacggcgggg gtaattatct acagtattat gaaacggtaa ttatcttcgt ggcattcggg 601 gggtaattat ctgccgtggg actcagaggt aattatctat atgtgttgaa actggtaatt 661 atctgctcga gatctgcgat ctgcatctca attagtcagc aaccatagtc ccgcccctaa 721 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catcgctgac 781 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt 841 agtgaggagg cttttttgga ggcctaggct tttgcaaagg atccgccacc atggattatg 901 gaggcgccct gagtgccgtt gggcgcgagc tgctatttgt aacgaaccca gtagtcgtca 961 atggctctgt acttgtgcct gaggaccagt gttactgcgc gggctggatt gagtcgcgtg 1021 gcacaaacgg tgcccaaacg gcgtcgaacg tgctgcaatg gcttgctgct ggcttctcca 1081 tcctactgct tatgttttac gcctaccaaa catggaagtc aacctgcggc tgggaggaga 1141 tctatgtgtg cgctatcgag atggtcaagg tgattctcga gttcttcttc gagtttaaga 1201 acccgtccat gctgtatcta gccacaggcc accgcgtcca gtggttgcgt tacgccgagt 1261 ggcttctcac ctgcccggtc atttgcattc acctgtcaaa cctgacgggc ttgtccaacg 1321 actacagcag gcgcaccatg ggtctgcttg tgtctgatat tggcacaatt gtgtggggcg 1381 ccacttccgc catggccacc ggatacgtca aggtcatctt cttctgcctg ggtctgtgtt 1441 atggtgctaa cacgttcttt cacgctgcca aggcctacat cgagggttac cacaccgtgc 1501 cgaagggccg gtgtcgccag gtggtgactg gcatggcttg gctcttcttc gtatcatggg 1561 gtatgttccc catcctgttc atcctcggcc ccgagggctt cggcgtcctg agcgtgtacg 1621 gctccaccgt cggccacacc atcattgacc tgatgtcgaa gaactgctgg ggtctgctcg 1681 gccactacct gcgcgtgctg atccacgagc atatcctcat ccacggcgac attcgcaaga 1741 ccaccaaatt gaacattggt ggcactgaga ttgaggtcga gacgctggtg gaggacgagg 1801 ccgaggctgg cgcggtaccc atggtgagca agggcgagga gctgttcacc ggggtggtgc 1861 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg 1921 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc 1981 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc 2041 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg 2101 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga 2161 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg 2221 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca 2281 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg 2341 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg 2401 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg 2461 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca 2521 tggacgagct gtacaagtga taagaattcg atatcaagct tatcgataat caacctctgg 2581 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat 2641 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt 2701 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca 2761 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg 2821 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg 2881 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca 2941 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tatgttgcca 3001 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc 3061 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc 3121 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgagcg ctgctcgaga 3181 gatctacggg tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc 3241 cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta 3301 ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg 3361 gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca 3421 atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc 3481 cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta 3541 gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta 3601 cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt 3661 ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg 3721 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc 3781 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg 3841 caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata 3901 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg 3961 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct 4021 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc 4081 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg 4141 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt 4201 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg 4261 gctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc 4321 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt 4381 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa 4441 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg 4501 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga 4561 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 4621 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 4681 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 4741 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 4801 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 4861 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 4921 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 4981 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 5041 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 5101 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 5161 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 5221 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 5281 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 5341 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 5401 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 5461 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 5521 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 5581 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 5641 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 5701 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 5761 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 5821 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 5881 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc 5941 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 6001 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 6061 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 6121 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 6181 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 6241 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 6301 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 6361 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 6421 gctggccttt tgctcacatg t //