LOCUS Exported 6541 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina. ACCESSION . VERSION . KEYWORDS 115_pAAV-ProC23-CatCh-GFP-WPRE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6541) AUTHORS Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B TITLE Targeting neuronal and glial cell types with synthetic promoter AAVs in mice, non-human primates and humans. JOURNAL Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 10.1038/s41593-019-0431-2. PUBMED 31285614 REFERENCE 2 (bases 1 to 6541) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6541 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..130 /label=AAV2 ITR (alternate) /note="Functional equivalent of wild-type AAV2 ITR" promoter 782..978 /label=SV40 promoter /note="SV40 early promoter" rep_origin 829..964 /label=SV40 ori /note="SV40 origin of replication" primer_bind 891..910 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" regulatory 985..994 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 991..1914 /codon_start=1 /product="N-terminal fragment of channelrhodopsin-2 from the alga Chlamydomonas reinhardtii (Nagel et al., 2003)" /label=ChR2 /note="blue light-activated cation channel" /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI RKTTKLNIGGTEIEVETLVEDEAEAGA" CDS 1921..2637 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(1966..1987) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(2227..2246) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 2574..2595 /label=EGFP-C /note="EGFP, forward primer" misc_feature 2668..3256 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2721..2741) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(3139..3150) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 3288..3764 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(3487..3506) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" repeat_region 3804..3944 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 3804..3933 /label=AAV2 ITR rep_origin 4019..4474 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4106..4125) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4316..4337 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(4491..4510) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 4610..4632 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(4670..4688) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 4756..4860 /gene="bla" /label=AmpR promoter CDS 4861..5721 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5079..5098) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5892..6480 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6381..6400 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggccgcac gcgtcgcggt tattccccta tggggtatca ataattttcg 181 ggccaactgc atggggtatc aataattcaa ttttgtagta tatggggtat caataattgg 241 tctcagtatt aaatggggta tcaataattc gaaccaatac ggcatggggt atcaataatc 301 aatcccaggc cgtaatgggg tatcaataat tgggattagc gctctatggg gtatcaataa 361 tccggcgtac cgatcaatgg ggtatcaata ataaagggat tattcacatg gggtatcaat 421 aataccagca aatgagacat ggggtatcaa taatcaagta gatgtcagta tggggtatca 481 ataattcaac acactgatga atggggtatc aataatgctt agatgtatcc tatggggtat 541 caataattga cgcaacaact ctatggggta tcaataatgg gaccgtagga ttcatggggt 601 atcaataata tctaaatctt ggcgatgggg tatcaataat ggtttctatc agacaatggg 661 gtatcaataa taaaaggtac tgggcgatgg ggtatcaata atgtccctag gcaccatatg 721 gggtatcaat aatgatcgca gccggttcat ggggtatcaa taatgctcga gatctgcgat 781 ctgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta 841 actccgccca gttccgccca ttctccgccc catcgctgac taattttttt tatttatgca 901 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 961 ggcctaggct tttgcaaagg atccgccacc atggattatg gaggcgccct gagtgccgtt 1021 gggcgcgagc tgctatttgt aacgaaccca gtagtcgtca atggctctgt acttgtgcct 1081 gaggaccagt gttactgcgc gggctggatt gagtcgcgtg gcacaaacgg tgcccaaacg 1141 gcgtcgaacg tgctgcaatg gcttgctgct ggcttctcca tcctactgct tatgttttac 1201 gcctaccaaa catggaagtc aacctgcggc tgggaggaga tctatgtgtg cgctatcgag 1261 atggtcaagg tgattctcga gttcttcttc gagtttaaga acccgtccat gctgtatcta 1321 gccacaggcc accgcgtcca gtggttgcgt tacgccgagt ggcttctcac ctgcccggtc 1381 atttgcattc acctgtcaaa cctgacgggc ttgtccaacg actacagcag gcgcaccatg 1441 ggtctgcttg tgtctgatat tggcacaatt gtgtggggcg ccacttccgc catggccacc 1501 ggatacgtca aggtcatctt cttctgcctg ggtctgtgtt atggtgctaa cacgttcttt 1561 cacgctgcca aggcctacat cgagggttac cacaccgtgc cgaagggccg gtgtcgccag 1621 gtggtgactg gcatggcttg gctcttcttc gtatcatggg gtatgttccc catcctgttc 1681 atcctcggcc ccgagggctt cggcgtcctg agcgtgtacg gctccaccgt cggccacacc 1741 atcattgacc tgatgtcgaa gaactgctgg ggtctgctcg gccactacct gcgcgtgctg 1801 atccacgagc atatcctcat ccacggcgac attcgcaaga ccaccaaatt gaacattggt 1861 ggcactgaga ttgaggtcga gacgctggtg gaggacgagg ccgaggctgg cgcggtaccc 1921 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 1981 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac 2041 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc 2101 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag 2161 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 2221 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 2281 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 2341 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac 2401 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc 2461 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac 2521 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc 2581 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtga 2641 taagaattcg atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga 2701 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 2761 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 2821 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 2881 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 2941 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 3001 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 3061 aaatcatcgt cctttccttg gctgctcgcc tatgttgcca cctggattct gcgcgggacg 3121 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 3181 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt 3241 tgggccgcct ccccgcatcg ataccgagcg ctgctcgaga gatctacggg tggcatccct 3301 gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc 3361 ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta 3421 tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct 3481 gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct 3541 ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg 3601 catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat 3661 tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat 3721 tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt ttgtaggtaa 3781 ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct 3841 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc 3901 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta 3961 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac 4021 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 4081 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 4141 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 4201 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca 4261 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 4321 ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa 4381 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 4441 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc 4501 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga 4561 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc 4621 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata 4681 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact 4741 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg 4801 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt 4861 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct 4921 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca 4981 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc 5041 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc 5101 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg 5161 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta 5221 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc 5281 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt 5341 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg 5401 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct 5461 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc 5521 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct 5581 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac 5641 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc 5701 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat 5761 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg 5821 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc 5881 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 5941 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 6001 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta 6061 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 6121 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 6181 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 6241 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 6301 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 6361 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 6421 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 6481 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 6541 t //