LOCUS Exported 6361 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina. ACCESSION . VERSION . KEYWORDS 116_pAAV-ProC24-CatCh-GFP-WPRE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6361) AUTHORS Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B TITLE Targeting neuronal and glial cell types with synthetic promoter AAVs in mice, non-human primates and humans. JOURNAL Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 10.1038/s41593-019-0431-2. PUBMED 31285614 REFERENCE 2 (bases 1 to 6361) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6361 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..130 /label=AAV2 ITR (alternate) /note="Functional equivalent of wild-type AAV2 ITR" promoter 602..798 /label=SV40 promoter /note="SV40 early promoter" rep_origin 649..784 /label=SV40 ori /note="SV40 origin of replication" primer_bind 711..730 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" regulatory 805..814 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 811..1734 /codon_start=1 /product="N-terminal fragment of channelrhodopsin-2 from the alga Chlamydomonas reinhardtii (Nagel et al., 2003)" /label=ChR2 /note="blue light-activated cation channel" /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI RKTTKLNIGGTEIEVETLVEDEAEAGA" CDS 1741..2457 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(1786..1807) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(2047..2066) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 2394..2415 /label=EGFP-C /note="EGFP, forward primer" misc_feature 2488..3076 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2541..2561) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(2959..2970) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 3108..3584 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(3307..3326) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" repeat_region 3624..3764 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 3624..3753 /label=AAV2 ITR rep_origin 3839..4294 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3926..3945) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4136..4157 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(4311..4330) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 4430..4452 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(4490..4508) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 4576..4680 /gene="bla" /label=AmpR promoter CDS 4681..5541 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4899..4918) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5712..6300 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6201..6220 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggccgcac gcgtaatcgg gaacacctaa aaacaaattt gaagggcaca 181 caaaacaaat gccagacccc tcaaaaacaa gtcacgtaac gggctaaaac aaccatgtag 241 atgtgttaaa acaacggtgc tgtaggccca aaacaaactc aactcacgat gaaaacaaat 301 ccaacggctg gtcaaaacaa tcacgggatc actcaaaaac aaaagaggtc ggggggtaaa 361 acaaagtgaa tgatttcgca aaacaaggtt cgtgtctgcg caaaacaatt tgagacataa 421 ttcaaaacaa gacacgggtg aatgaaaaac aaaaattcca acctgggaaa acaacccatg 481 caatatttaa aaacaagcca attgctggtt taaaacaagg ggtgggtagc ccgaaaacaa 541 gcaatggacg ttttaaaaac aatgcctgcc gctgacaaaa acaagctcga gatctgcgat 601 ctgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta 661 actccgccca gttccgccca ttctccgccc catcgctgac taattttttt tatttatgca 721 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 781 ggcctaggct tttgcaaagg atccgccacc atggattatg gaggcgccct gagtgccgtt 841 gggcgcgagc tgctatttgt aacgaaccca gtagtcgtca atggctctgt acttgtgcct 901 gaggaccagt gttactgcgc gggctggatt gagtcgcgtg gcacaaacgg tgcccaaacg 961 gcgtcgaacg tgctgcaatg gcttgctgct ggcttctcca tcctactgct tatgttttac 1021 gcctaccaaa catggaagtc aacctgcggc tgggaggaga tctatgtgtg cgctatcgag 1081 atggtcaagg tgattctcga gttcttcttc gagtttaaga acccgtccat gctgtatcta 1141 gccacaggcc accgcgtcca gtggttgcgt tacgccgagt ggcttctcac ctgcccggtc 1201 atttgcattc acctgtcaaa cctgacgggc ttgtccaacg actacagcag gcgcaccatg 1261 ggtctgcttg tgtctgatat tggcacaatt gtgtggggcg ccacttccgc catggccacc 1321 ggatacgtca aggtcatctt cttctgcctg ggtctgtgtt atggtgctaa cacgttcttt 1381 cacgctgcca aggcctacat cgagggttac cacaccgtgc cgaagggccg gtgtcgccag 1441 gtggtgactg gcatggcttg gctcttcttc gtatcatggg gtatgttccc catcctgttc 1501 atcctcggcc ccgagggctt cggcgtcctg agcgtgtacg gctccaccgt cggccacacc 1561 atcattgacc tgatgtcgaa gaactgctgg ggtctgctcg gccactacct gcgcgtgctg 1621 atccacgagc atatcctcat ccacggcgac attcgcaaga ccaccaaatt gaacattggt 1681 ggcactgaga ttgaggtcga gacgctggtg gaggacgagg ccgaggctgg cgcggtaccc 1741 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 1801 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac 1861 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc 1921 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag 1981 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 2041 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 2101 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 2161 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac 2221 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc 2281 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac 2341 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc 2401 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtga 2461 taagaattcg atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga 2521 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 2581 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 2641 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 2701 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 2761 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 2821 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 2881 aaatcatcgt cctttccttg gctgctcgcc tatgttgcca cctggattct gcgcgggacg 2941 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 3001 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt 3061 tgggccgcct ccccgcatcg ataccgagcg ctgctcgaga gatctacggg tggcatccct 3121 gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc 3181 ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta 3241 tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct 3301 gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct 3361 ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg 3421 catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat 3481 tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat 3541 tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt ttgtaggtaa 3601 ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct 3661 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc 3721 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta 3781 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac 3841 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 3901 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 3961 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 4021 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca 4081 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 4141 ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa 4201 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 4261 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc 4321 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga 4381 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc 4441 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata 4501 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact 4561 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg 4621 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt 4681 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct 4741 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca 4801 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc 4861 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc 4921 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg 4981 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta 5041 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc 5101 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt 5161 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg 5221 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct 5281 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc 5341 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct 5401 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac 5461 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc 5521 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat 5581 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg 5641 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc 5701 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 5761 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 5821 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta 5881 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 5941 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 6001 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 6061 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 6121 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 6181 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 6241 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 6301 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 6361 t //