LOCUS Exported 6401 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina. ACCESSION . VERSION . KEYWORDS 124_pAAV-ProC3-CatCh-GFP-WPRE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6401) AUTHORS Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B TITLE Targeting neuronal and glial cell types with synthetic promoter AAVs in mice, non-human primates and humans. JOURNAL Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 10.1038/s41593-019-0431-2. PUBMED 31285614 REFERENCE 2 (bases 1 to 6401) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6401 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..130 /label=AAV2 ITR (alternate) /note="Functional equivalent of wild-type AAV2 ITR" promoter 642..838 /label=SV40 promoter /note="SV40 early promoter" rep_origin 689..824 /label=SV40 ori /note="SV40 origin of replication" primer_bind 751..770 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" regulatory 845..854 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 851..1774 /codon_start=1 /product="N-terminal fragment of channelrhodopsin-2 from the alga Chlamydomonas reinhardtii (Nagel et al., 2003)" /label=ChR2 /note="blue light-activated cation channel" /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI RKTTKLNIGGTEIEVETLVEDEAEAGA" CDS 1781..2497 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(1826..1847) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(2087..2106) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 2434..2455 /label=EGFP-C /note="EGFP, forward primer" misc_feature 2528..3116 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2581..2601) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(2999..3010) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 3148..3624 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(3347..3366) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" repeat_region 3664..3804 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 3664..3793 /label=AAV2 ITR rep_origin 3879..4334 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3966..3985) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4176..4197 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(4351..4370) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 4470..4492 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(4530..4548) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 4616..4720 /gene="bla" /label=AmpR promoter CDS 4721..5581 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4939..4958) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5752..6340 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6241..6260 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggccgcac gcgtaaagct ggaactgggg ccggaaacag ctgagggtgc 181 ccagccggaa actcgaaatc aacgtaggcc ggaaactatt cgatgaattc cgccggaaac 241 taggtccgga ggactgccgg aaacacctgt aatcaagccg ccggaaacct gttgtggccg 301 tatgccggaa acgtcttaat tggacgtgcc ggaaactctt ttaatgagtt cgccggaaac 361 cagaccagcc gagctgccgg aaaccggtta tatagaacgg ccggaaacgg tccacaggaa 421 aaagccggaa acacccaaac ggttagcgcc ggaaacgact ggggaggacg tgccggaaac 481 gtactatctg aagatgccgg aaacacttga aagctccaag ccggaaacgg gcccgtgcgg 541 atagccggaa actgacggta cacggccgcc ggaaacacta cttgtatggt agccggaaac 601 ttgggcgtgg ctggggccgg aaacgctcga gatctgcgat ctgcatctca attagtcagc 661 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 721 ttctccgccc catcgctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 781 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaagg 841 atccgccacc atggattatg gaggcgccct gagtgccgtt gggcgcgagc tgctatttgt 901 aacgaaccca gtagtcgtca atggctctgt acttgtgcct gaggaccagt gttactgcgc 961 gggctggatt gagtcgcgtg gcacaaacgg tgcccaaacg gcgtcgaacg tgctgcaatg 1021 gcttgctgct ggcttctcca tcctactgct tatgttttac gcctaccaaa catggaagtc 1081 aacctgcggc tgggaggaga tctatgtgtg cgctatcgag atggtcaagg tgattctcga 1141 gttcttcttc gagtttaaga acccgtccat gctgtatcta gccacaggcc accgcgtcca 1201 gtggttgcgt tacgccgagt ggcttctcac ctgcccggtc atttgcattc acctgtcaaa 1261 cctgacgggc ttgtccaacg actacagcag gcgcaccatg ggtctgcttg tgtctgatat 1321 tggcacaatt gtgtggggcg ccacttccgc catggccacc ggatacgtca aggtcatctt 1381 cttctgcctg ggtctgtgtt atggtgctaa cacgttcttt cacgctgcca aggcctacat 1441 cgagggttac cacaccgtgc cgaagggccg gtgtcgccag gtggtgactg gcatggcttg 1501 gctcttcttc gtatcatggg gtatgttccc catcctgttc atcctcggcc ccgagggctt 1561 cggcgtcctg agcgtgtacg gctccaccgt cggccacacc atcattgacc tgatgtcgaa 1621 gaactgctgg ggtctgctcg gccactacct gcgcgtgctg atccacgagc atatcctcat 1681 ccacggcgac attcgcaaga ccaccaaatt gaacattggt ggcactgaga ttgaggtcga 1741 gacgctggtg gaggacgagg ccgaggctgg cgcggtaccc atggtgagca agggcgagga 1801 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa 1861 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt 1921 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta 1981 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc 2041 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta 2101 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa 2161 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa 2221 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa 2281 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac 2341 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc 2401 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc 2461 cgccgggatc actctcggca tggacgagct gtacaagtga taagaattcg atatcaagct 2521 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta 2581 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc 2641 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga 2701 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac 2761 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc 2821 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc 2881 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg 2941 gctgctcgcc tatgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc 3001 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc 3061 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg 3121 ataccgagcg ctgctcgaga gatctacggg tggcatccct gtgacccctc cccagtgcct 3181 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt 3241 tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta 3301 tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca 3361 agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg 3421 attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc 3481 taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac 3541 tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga 3601 accactgctc ccttccctgt ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc 3661 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg 3721 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg 3781 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt 3841 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt 3901 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 3961 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 4021 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc 4081 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt 4141 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac 4201 aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc 4261 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt 4321 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag 4381 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc 4441 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc 4501 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa 4561 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg 4621 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata 4681 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg 4741 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac 4801 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact 4861 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat 4921 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga 4981 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac 5041 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat 5101 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac 5161 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct 5221 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac 5281 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga 5341 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg 5401 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact 5461 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac 5521 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta 5581 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt 5641 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga 5701 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc 5761 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt 5821 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc 5881 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc 5941 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg 6001 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg 6061 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga 6121 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc 6181 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg 6241 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg 6301 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt 6361 tttacggttc ctggcctttt gctggccttt tgctcacatg t //