LOCUS Exported 11767 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of XRN1__PRKCE fusion. ACCESSION . VERSION . KEYWORDS pHAGE-XRN1__PRKCE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11767) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 11767) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11767 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2058..2175 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2223..3401 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2454..3392 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3349..3369 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 3421..3445 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(6649..6673) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(6827..6847) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 6901..6942 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 6963..7530 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 6980..7532 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(7124..7141) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 7351..7370 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 7544..8143 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(7544..7563) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 8040..8060 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 8157..8745 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(8210..8230) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(8628..8639) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 8820..9053 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(9172..9190) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 9258..9362 /gene="bla" /label=AmpR promoter CDS 9363..10223 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(9581..9600) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 10394..10982 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 10883..10902 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 11136..11153 /label=L4440 /note="L4440 vector, forward primer" primer_bind 11245..11264 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 11393..11414 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 11429..11459 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 11467..11483 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 11472..11494 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 11491..11507 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 11491..11507 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 11557..11576 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt 2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca 3421 acaagtttgt acaaaaaagt tggcatggga gtccccaagt tttacagatg gatctcagag 3481 cggtatccct gtctcagcga agtggtgaaa gagcatcaga ttcctgaatt tgacaacttg 3541 tacctggata tgaatggaat tatacatcag tgctcccatc ctaatgatga tgatgttcac 3601 tttagaattt cagatgataa aatctttact gatatttttc actacctgga ggtgttgttt 3661 cgcattatta aacccaggaa agtgttcttt atggctgtag atggtgtggc tcctcgagca 3721 aaaatgaacc agcagcgtgg gaggcgtttt aggtcagcaa aggaggcaga agacaaaatt 3781 aaaaaggcaa tagagaaggg agaaactctt cctacagagg ccagatttga ttccaactgt 3841 atcacaccag gaactgaatt tatggccagg ttacatgaac atctgaagta ttttgtaaat 3901 atgaaaattt ccacagacaa gtcatggcaa ggagttacca tctacttctc aggccatgag 3961 actcctggag aaggagagca taaaatcatg gaatttatca gatccgagaa agcaaagcca 4021 gatcatgatc caaacaccag acactgtctt tatggtttag atgctgactt gattatgctt 4081 ggattaacaa gtcatgaggc acatttttct ctcttaagag aagaagttcg atttggtggc 4141 aaaaaaacac aacgggtatg tgctccagaa gaaactacat ttcaccttct acacttgtct 4201 ttaatgagag agtatattga ctatgagttt tcagtattaa aagaaaagat cacatttaaa 4261 tatgatattg aaaggataat agatgattgg attttgatgg ggtttcttgt tggtaatgat 4321 tttatccctc atctacctca tttacatatt aatcatgatg cactgcctct tctttatgga 4381 acatatgtta ccatcctgcc agaacttggg ggttatatta atgaaagtgg gcacctcaac 4441 ttacctcgat ttgagaaata ccttgtgaaa ctatcagatt ttgatcggga gcacttcagt 4501 gaagtttttg tggacctaaa atggtttgaa agcaaagttg gtaacaagta cctcaatgaa 4561 gcagcaggtg tcgcagcaga agaagccagg aactacaagg aaaagaaaaa gttaaagggc 4621 caggaaaatt ctctgtgttg gactgcttta gacaaaaatg aaggcgaaat gataacttct 4681 aaggataatt tagaagatga gactgaagat gatgacctat ttgaaactga gtttagacaa 4741 tataaaagaa catattacat gacgaagatg ggggttgacg tagtatctga tgactttctg 4801 gctgatcaag ctgcatgtta tgttcaggca atacagtgga ttttgcacta ttactatcat 4861 ggagttcagt cctggagctg gtattatcct tatcattatg cacctttcct gtctgatata 4921 cacaacatca gtacactcaa aatccatttt gaactaggaa aaccttttaa gccatttgaa 4981 cagcttcttg ctgtacttcc agcagccagc aaaaatttac ttcctgcatg ctaccagcat 5041 ttgatgacca atgaagactc accaattata gaatattacc cacctgattt taaaactgac 5101 ctaaatggga aacaacagga atgggaagct gtggtgttaa tcccttttat tgatgagaag 5161 cgattattgg aagccatgga gacatgtaac cactccctca aaaaggaaga gaggaaaaga 5221 aaccaacata gtgagtgcct aatgtgctgg tatgatagag acacagagtt tatctatcct 5281 tctccatggc cagaaaagtt ccctgccata gaacgatgtt gtacaaggta taaaataata 5341 tccttagatg cttggcgtgt agacataaac aaaaacaaaa taaccagaat tgaccagaaa 5401 gcattatatt tctgtggatt tcctactctg aaacacatca gacacaaatt ttttttgaag 5461 aaaagtggtg ttcaagtatt ccagcaaagc agtcgtggag aaaacatgat gttggaaatc 5521 ttagtggatg cagaatcaga tgaacttacc gtagaaaatg tagcttcatc agtgcttgga 5581 aaatctgtct ttgttaattg gcctcacctt gaggaagcta gagtcgtggc tgtatcagat 5641 ggagaaacta agttttactt ggaagaacct ccaggaacac agaagcttta ttcaggaaga 5701 actgccccac catctaaagt ggttcatctt ggagataaag aacaatctaa ctgggcaaaa 5761 gaagtacaag gaatttcaga acactacctg agaagaaaag gaataataat aaatgaaaca 5821 tctgcagttg tgtatgctca gttactcaca ggtcgtaaat atcaaataaa tcaaaatggt 5881 gaagttcgtc tagagaaaca gtggtcaaaa caagttgttc cttttgttta tcaaactatt 5941 gtcaaggaca tccgagcttt cgactcccgt ttctccaata tcaaaacatt ggatgatttg 6001 tttcctctga gaagtatggt ctttatgctg ggaactccct attatggctg cactggagaa 6061 gttcaggatt caggtgatgt gattacagaa ggtaggattc gtgtgatttt cagcattcca 6121 tgtgaaccca atcttgatgc tttaatacag aaccagcata aatattctat aaagtacaac 6181 ccaggatatg tgttggccag tcgccttgga gtgagtggat accttgtttc aaggtttaca 6241 ggaagtattt ttattggaag aggatctagg agaaaccctc atggagacca taaagcaaat 6301 gtgggtttaa atctcaaatt caacaagaaa aatgaggagg tacctggata tactaagaaa 6361 gttggaagtg aatggatgta ttcatctgca gcagaacaac ttctggcaga gtacttagag 6421 agagctccag aactatttag ttatatagcc aaaaatagcc aagaggatgt gttctatgaa 6481 gatgacattt ggcctggaga aaatgagaat ggtgctgaaa aagttcaaga aattattact 6541 tggctaaaag gacatcctgt cagtacttta tctcgttctt cttgtgattt acaaattctg 6601 gatgcagcta ttgttgagaa aattgaggaa gaagtcgaaa agtgcaagtc ccagctttct 6661 tgtacaaagt ggttcaaaac caaaagagac gtcaataatt ttgaccaaga ctttacccgg 6721 gaagagccgg tactcaccct tgtggacgaa gcaattgtaa agcagatcaa ccaggaggaa 6781 ttcaaaggtt tctcctactt tggtgaagac ctgatgccct gacacccaac ttttctatac 6841 aaagtggttg atatccagca cagtggcggc cgctcgagtc tagagggccc gcggttcgaa 6901 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg ttagtaatga 6961 gatccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg 7021 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa 7081 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg 7141 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca 7201 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc 7261 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt 7321 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg 7381 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca 7441 tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac 7501 gtggttttcc tttgaaaaac acgatgataa tatggccaca catatgaccg agtacaagcc 7561 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc 7621 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt 7681 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt 7741 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc 7801 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca 7861 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc 7921 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc 7981 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg 8041 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga 8101 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc taaatcgata gatcctaatc 8161 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt 8221 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg 8281 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc 8341 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt 8401 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg 8461 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg 8521 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct 8581 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc 8641 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc 8701 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgag atcctttaag 8761 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact 8821 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct 8881 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 8941 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 9001 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag 9061 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 9121 gaggcccggg ttaattaagg aaagggctag atcattcttg aagacgaaag ggcctcgtga 9181 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca 9241 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata 9301 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga 9361 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc 9421 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg 9481 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc 9541 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat 9601 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact 9661 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat 9721 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga 9781 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc 9841 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga 9901 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag 9961 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc 10021 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt 10081 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct 10141 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg 10201 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg 10261 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca 10321 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga 10381 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 10441 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 10501 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt 10561 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 10621 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 10681 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 10741 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 10801 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 10861 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 10921 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 10981 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 11041 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag 11101 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg 11161 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagca 11221 agctcatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 11281 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc 11341 cgtggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga 11401 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt 11461 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg attacgaatt 11521 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 11581 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc 11641 ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg 11701 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact 11761 tagtagt //