LOCUS       Exported                6179 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    157_pAAV-ProD2-CatCh-GFP-WPRE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6179)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6179
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        532..563
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      623..632
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             629..1552
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGA"
     CDS             1559..2275
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1604..1625)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1865..1884)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2212..2233
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2306..2894
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2359..2379)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2777..2788)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2926..3402
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3125..3144)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3442..3582
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3442..3571
                     /label=AAV2 ITR
     rep_origin      3657..4112
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3744..3763)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3954..3975
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4129..4148)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4248..4270
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4308..4326)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4394..4498
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4499..5359
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4717..4736)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5530..6118
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6019..6038
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtacgcgt atcctggggt ttaaacagga ctaggtccac
      181 tgggagaaga ggaagtcaaa caggactagt catcgatagg ctagccagct taattaatca
      241 gatcccttcc agcatgacaa taccaggttt tcaagggccc tagaactatg agatggtaaa
      301 gtgatgcccc cagatgtcct actcacagca catgcagctg cttctctctc ccggctctcc
      361 cctcaagagg cagctatgca caggaatagt ctaataatcc caaagtcacc taaatactaa
      421 tacaattaag actgtaacag ttaatcccag acagcactct gggcggggct agtctcgtac
      481 atggaaaggt cagtgaaagc ctctatcctc acatggtcct gctggagtta gtagagggta
      541 tataatggaa gctcgacttc cagctatcac atccactgtg ttgttgtgaa ctggaatcca
      601 ctataggcca ggatccggat ccgccaccat ggattatgga ggcgccctga gtgccgttgg
      661 gcgcgagctg ctatttgtaa cgaacccagt agtcgtcaat ggctctgtac ttgtgcctga
      721 ggaccagtgt tactgcgcgg gctggattga gtcgcgtggc acaaacggtg cccaaacggc
      781 gtcgaacgtg ctgcaatggc ttgctgctgg cttctccatc ctactgctta tgttttacgc
      841 ctaccaaaca tggaagtcaa cctgcggctg ggaggagatc tatgtgtgcg ctatcgagat
      901 ggtcaaggtg attctcgagt tcttcttcga gtttaagaac ccgtccatgc tgtatctagc
      961 cacaggccac cgcgtccagt ggttgcgtta cgccgagtgg cttctcacct gcccggtcat
     1021 ttgcattcac ctgtcaaacc tgacgggctt gtccaacgac tacagcaggc gcaccatggg
     1081 tctgcttgtg tctgatattg gcacaattgt gtggggcgcc acttccgcca tggccaccgg
     1141 atacgtcaag gtcatcttct tctgcctggg tctgtgttat ggtgctaaca cgttctttca
     1201 cgctgccaag gcctacatcg agggttacca caccgtgccg aagggccggt gtcgccaggt
     1261 ggtgactggc atggcttggc tcttcttcgt atcatggggt atgttcccca tcctgttcat
     1321 cctcggcccc gagggcttcg gcgtcctgag cgtgtacggc tccaccgtcg gccacaccat
     1381 cattgacctg atgtcgaaga actgctgggg tctgctcggc cactacctgc gcgtgctgat
     1441 ccacgagcat atcctcatcc acggcgacat tcgcaagacc accaaattga acattggtgg
     1501 cactgagatt gaggtcgaga cgctggtgga ggacgaggcc gaggctggcg cggtacccat
     1561 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1621 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     1681 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1741 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     1801 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     1861 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     1921 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     1981 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     2041 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     2101 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     2161 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     2221 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtgata
     2281 agaattcgat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
     2341 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     2401 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     2461 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     2521 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     2581 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     2641 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     2701 atcatcgtcc tttccttggc tgctcgccta tgttgccacc tggattctgc gcgggacgtc
     2761 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     2821 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     2881 ggccgcctcc ccgcatcgat accgagcgct gctcgagaga tctacgggtg gcatccctgt
     2941 gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt
     3001 gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta taatattatg
     3061 gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc
     3121 ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc
     3181 gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca
     3241 tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg
     3301 gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg
     3361 ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgatttt gtaggtaacc
     3421 acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc
     3481 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc
     3541 gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt
     3601 ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc
     3661 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     3721 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     3781 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     3841 tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc
     3901 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     3961 cttgttccaa actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg
     4021 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     4081 gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc
     4141 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     4201 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     4261 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     4321 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     4381 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     4441 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     4501 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     4561 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     4621 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     4681 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     4741 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     4801 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     4861 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     4921 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     4981 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     5041 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     5101 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     5161 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     5221 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     5281 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     5341 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     5401 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     5461 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     5521 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     5581 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     5641 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     5701 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     5761 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     5821 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     5881 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     5941 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6001 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6061 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6121 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt
//
