LOCUS       Exported                6285 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    172_pAAV-ProC37-CatCh-GFP-WPRE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6285)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6285
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        526..722
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      573..708
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     635..654
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     regulatory      729..738
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             735..1658
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGA"
     CDS             1665..2381
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1710..1731)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1971..1990)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2318..2339
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2412..3000
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2465..2485)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2883..2894)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3032..3508
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3231..3250)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3548..3688
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3548..3677
                     /label=AAV2 ITR
     rep_origin      3763..4218
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3850..3869)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4060..4081
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4235..4254)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4354..4376
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4414..4432)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4500..4604
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4605..5465
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4823..4842)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5636..6224
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6125..6144
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtccgtgg cccatacgct gccctaaggc agaggatcgc
      181 gggccataca cctgtcggat catgatttcc ttgccctaag gcacgctgta atacctttta
      241 cacctgtcct tccgtctacg agatgcccta aggcaatggt ggtcacccag tacacctgtc
      301 ttggagcgag cgcagtgccc taaggcacgg tgcactagcc gttacacctg tcatcgtgcg
      361 ctgcatttgc cctaaggcag tcaatggagg attgtacacc tgtcccctta ctcaagaaat
      421 gccctaaggc aggcgcgtgc atgacgtaca cctgtctctc tcaccaccaa atgccctaag
      481 gcatcacttg cgtctgcgta cacctgtcgc tcgagatctg cgatctgcat ctcaattagt
      541 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
      601 cccattctcc gccccatcgc tgactaattt tttttattta tgcagaggcc gaggccgcct
      661 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
      721 aaggatccgc caccatggat tatggaggcg ccctgagtgc cgttgggcgc gagctgctat
      781 ttgtaacgaa cccagtagtc gtcaatggct ctgtacttgt gcctgaggac cagtgttact
      841 gcgcgggctg gattgagtcg cgtggcacaa acggtgccca aacggcgtcg aacgtgctgc
      901 aatggcttgc tgctggcttc tccatcctac tgcttatgtt ttacgcctac caaacatgga
      961 agtcaacctg cggctgggag gagatctatg tgtgcgctat cgagatggtc aaggtgattc
     1021 tcgagttctt cttcgagttt aagaacccgt ccatgctgta tctagccaca ggccaccgcg
     1081 tccagtggtt gcgttacgcc gagtggcttc tcacctgccc ggtcatttgc attcacctgt
     1141 caaacctgac gggcttgtcc aacgactaca gcaggcgcac catgggtctg cttgtgtctg
     1201 atattggcac aattgtgtgg ggcgccactt ccgccatggc caccggatac gtcaaggtca
     1261 tcttcttctg cctgggtctg tgttatggtg ctaacacgtt ctttcacgct gccaaggcct
     1321 acatcgaggg ttaccacacc gtgccgaagg gccggtgtcg ccaggtggtg actggcatgg
     1381 cttggctctt cttcgtatca tggggtatgt tccccatcct gttcatcctc ggccccgagg
     1441 gcttcggcgt cctgagcgtg tacggctcca ccgtcggcca caccatcatt gacctgatgt
     1501 cgaagaactg ctggggtctg ctcggccact acctgcgcgt gctgatccac gagcatatcc
     1561 tcatccacgg cgacattcgc aagaccacca aattgaacat tggtggcact gagattgagg
     1621 tcgagacgct ggtggaggac gaggccgagg ctggcgcggt acccatggtg agcaagggcg
     1681 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     1741 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     1801 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     1861 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     1921 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     1981 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     2041 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     2101 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     2161 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     2221 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     2281 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     2341 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtgataagaa ttcgatatca
     2401 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     2461 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     2521 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     2581 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     2641 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     2701 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     2761 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     2821 cttggctgct cgcctatgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     2881 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     2941 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     3001 atcgataccg agcgctgctc gagagatcta cgggtggcat ccctgtgacc cctccccagt
     3061 gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt
     3121 aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt
     3181 ggtatggagc aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga
     3241 accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca
     3301 agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct
     3361 cagctaattt ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc
     3421 caactcctaa tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc
     3481 gtgaaccact gctcccttcc ctgtccttct gattttgtag gtaaccacgt gcggaccgag
     3541 cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
     3601 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
     3661 agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat
     3721 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
     3781 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     3841 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     3901 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     3961 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     4021 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     4081 gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
     4141 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     4201 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     4261 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     4321 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     4381 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     4441 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     4501 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     4561 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     4621 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     4681 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     4741 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     4801 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     4861 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     4921 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     4981 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     5041 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     5101 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     5161 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     5221 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     5281 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     5341 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     5401 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     5461 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     5521 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     5581 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     5641 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     5701 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5761 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     5821 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     5881 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     5941 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6001 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6061 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6121 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6181 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6241 cctttttacg gttcctggcc ttttgctggc cttttgctca catgt
//
