LOCUS Exported 6408 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina. ACCESSION . VERSION . KEYWORDS 180_pAAV-ProD11-CatCh-GFP-WPRE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6408) AUTHORS Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B TITLE Targeting neuronal and glial cell types with synthetic promoter AAVs in mice, non-human primates and humans. JOURNAL Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 10.1038/s41593-019-0431-2. PUBMED 31285614 REFERENCE 2 (bases 1 to 6408) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6408 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..130 /label=AAV2 ITR (alternate) /note="Functional equivalent of wild-type AAV2 ITR" promoter 767..798 /label=minP /note="minimal TATA-box promoter with low basal activity" regulatory 852..861 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 858..1781 /codon_start=1 /product="N-terminal fragment of channelrhodopsin-2 from the alga Chlamydomonas reinhardtii (Nagel et al., 2003)" /label=ChR2 /note="blue light-activated cation channel" /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI RKTTKLNIGGTEIEVETLVEDEAEAGA" CDS 1788..2504 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(1833..1854) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(2094..2113) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 2441..2462 /label=EGFP-C /note="EGFP, forward primer" misc_feature 2535..3123 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2588..2608) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(3006..3017) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 3155..3631 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(3354..3373) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" repeat_region 3671..3811 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 3671..3800 /label=AAV2 ITR rep_origin 3886..4341 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3973..3992) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4183..4204 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(4358..4377) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 4477..4499 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(4537..4555) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 4623..4727 /gene="bla" /label=AmpR promoter CDS 4728..5588 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4946..4965) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5759..6347 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6248..6267 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggccgcac gcgtatcctg gggtttaaac aggactaggt ccactgggag 181 aagaggaagt caaacaggac tagtcatcga taggctagcc agcttaatta atcagatgca 241 ggagtttgag ggatgcggat gaataggttg agggttgagg accactgcgc ctctgatggt 301 tgtctagagg cagacaatac gcagtctgga gctggagcag gtgcgtgcag tgtgcattgg 361 ggcttctgcc agtgatccaa gataggaaga catagtgagt gcttagaaga gcaccagcat 421 gtgaagggct gagaagtggt cggcgaggaa aggccctcaa aggagcctca gagggagtgg 481 acacagagag gcttaaagtt aagagcatcg agaagacagc tcttcgtgga aggggtgtgg 541 cctccagtta ggttgttggg gaattgtaga gataaatgaa agtatccgtt agacttgcaa 601 aggcgatgac aaaaataaac ctggttaatg ctgtcagtgg tttgaaagtg agcaagaaga 661 ttgcaccaga ttaaagagca agtgagcagt ctgttaaatg tacatgtctg ttggtaggag 721 catggccacc cttgaagcaa tcctcacatg gtcctgctgg agttagtaga gggtatataa 781 tggaagctcg acttccagct atcacatcca ctgtgttgtt gtgaactgga atccactata 841 ggccaggatc cgccaccatg gattatggag gcgccctgag tgccgttggg cgcgagctgc 901 tatttgtaac gaacccagta gtcgtcaatg gctctgtact tgtgcctgag gaccagtgtt 961 actgcgcggg ctggattgag tcgcgtggca caaacggtgc ccaaacggcg tcgaacgtgc 1021 tgcaatggct tgctgctggc ttctccatcc tactgcttat gttttacgcc taccaaacat 1081 ggaagtcaac ctgcggctgg gaggagatct atgtgtgcgc tatcgagatg gtcaaggtga 1141 ttctcgagtt cttcttcgag tttaagaacc cgtccatgct gtatctagcc acaggccacc 1201 gcgtccagtg gttgcgttac gccgagtggc ttctcacctg cccggtcatt tgcattcacc 1261 tgtcaaacct gacgggcttg tccaacgact acagcaggcg caccatgggt ctgcttgtgt 1321 ctgatattgg cacaattgtg tggggcgcca cttccgccat ggccaccgga tacgtcaagg 1381 tcatcttctt ctgcctgggt ctgtgttatg gtgctaacac gttctttcac gctgccaagg 1441 cctacatcga gggttaccac accgtgccga agggccggtg tcgccaggtg gtgactggca 1501 tggcttggct cttcttcgta tcatggggta tgttccccat cctgttcatc ctcggccccg 1561 agggcttcgg cgtcctgagc gtgtacggct ccaccgtcgg ccacaccatc attgacctga 1621 tgtcgaagaa ctgctggggt ctgctcggcc actacctgcg cgtgctgatc cacgagcata 1681 tcctcatcca cggcgacatt cgcaagacca ccaaattgaa cattggtggc actgagattg 1741 aggtcgagac gctggtggag gacgaggccg aggctggcgc ggtacccatg gtgagcaagg 1801 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg 1861 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc 1921 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc 1981 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct 2041 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg 2101 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg 2161 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca 2221 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga 2281 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc 2341 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc 2401 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg 2461 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtgataa gaattcgata 2521 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc 2581 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg 2641 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc 2701 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg 2761 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg 2821 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga 2881 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct 2941 ttccttggct gctcgcctat gttgccacct ggattctgcg cgggacgtcc ttctgctacg 3001 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc 3061 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc 3121 cgcatcgata ccgagcgctg ctcgagagat ctacgggtgg catccctgtg acccctcccc 3181 agtgcctctc ctggccctgg aagttgccac tccagtgccc accagccttg tcctaataaa 3241 attaagttgc atcattttgt ctgactaggt gtccttctat aatattatgg ggtggagggg 3301 ggtggtatgg agcaaggggc aagttgggaa gacaacctgt agggcctgcg gggtctattg 3361 ggaaccaagc tggagtgcag tggcacaatc ttggctcact gcaatctccg cctcctgggt 3421 tcaagcgatt ctcctgcctc agcctcccga gttgttggga ttccaggcat gcatgaccag 3481 gctcagctaa tttttgtttt tttggtagag acggggtttc accatattgg ccaggctggt 3541 ctccaactcc taatctcagg tgatctaccc accttggcct cccaaattgc tgggattaca 3601 ggcgtgaacc actgctccct tccctgtcct tctgattttg taggtaacca cgtgcggacc 3661 gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg 3721 ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca 3781 gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg 3841 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg 3901 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 3961 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 4021 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc 4081 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga 4141 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 4201 ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg attttgccga 4261 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca 4321 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat 4381 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc 4441 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt 4501 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat 4561 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg 4621 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 4681 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 4741 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 4801 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 4861 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 4921 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg 4981 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 5041 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 5101 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 5161 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 5221 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg 5281 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 5341 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 5401 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 5461 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 5521 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 5581 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 5641 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 5701 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 5761 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 5821 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 5881 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca 5941 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 6001 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 6061 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 6121 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 6181 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 6241 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 6301 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 6361 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt //