LOCUS       Exported                6392 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    181_pAAV-ProD12-CatCh-GFP-WPRE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6392)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6392)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6392
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        751..782
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      836..845
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             842..1765
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGA"
     CDS             1772..2488
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1817..1838)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2078..2097)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2425..2446
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2519..3107
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2572..2592)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2990..3001)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3139..3615
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3338..3357)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3655..3795
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3655..3784
                     /label=AAV2 ITR
     rep_origin      3870..4325
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3957..3976)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4167..4188
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4342..4361)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4461..4483
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4521..4539)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4607..4711
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4712..5572
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4930..4949)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5743..6331
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6232..6251
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtatcctg gggtttaaac aggactaggt ccactgggag
      181 aagaggaagt caaacaggac tagtcatcga taggctagcc agcttaatta atcagattct
      241 caacttacat ccttccacct cccaactaaa agcttgcgtg tgaagcacaa gagagtaagc
      301 ctcatgcccc gctctcaccc ttgccctgga atcagcagct atctgtgggc aatccgatct
      361 ggaggcctgc cctcatagcc accagggatc ttgttccttc ctcaccgtct gccctccact
      421 gggggttaca cgtgtgaccc tgagtgttgt ctgcccggta ctcatgggtc taggtcagag
      481 aaagaaacct ctgagtaaag cagtctgtgc accagagttt tctagaaaca aaagctccca
      541 ggaagttttt ctcttcagaa tgagtatctc cagcccatct ctagcctgac cagatgcctg
      601 caaataaaga gagctgtgaa agaaagaacc aaataaaaga aacacaaaag aatttgggcc
      661 cttgctgact tctgctcccc tggaccaccc gttgcaacag cagagccagg caactacagt
      721 accatcctca catggtcctg ctggagttag tagagggtat ataatggaag ctcgacttcc
      781 agctatcaca tccactgtgt tgttgtgaac tggaatccac tataggccag gatccgccac
      841 catggattat ggaggcgccc tgagtgccgt tgggcgcgag ctgctatttg taacgaaccc
      901 agtagtcgtc aatggctctg tacttgtgcc tgaggaccag tgttactgcg cgggctggat
      961 tgagtcgcgt ggcacaaacg gtgcccaaac ggcgtcgaac gtgctgcaat ggcttgctgc
     1021 tggcttctcc atcctactgc ttatgtttta cgcctaccaa acatggaagt caacctgcgg
     1081 ctgggaggag atctatgtgt gcgctatcga gatggtcaag gtgattctcg agttcttctt
     1141 cgagtttaag aacccgtcca tgctgtatct agccacaggc caccgcgtcc agtggttgcg
     1201 ttacgccgag tggcttctca cctgcccggt catttgcatt cacctgtcaa acctgacggg
     1261 cttgtccaac gactacagca ggcgcaccat gggtctgctt gtgtctgata ttggcacaat
     1321 tgtgtggggc gccacttccg ccatggccac cggatacgtc aaggtcatct tcttctgcct
     1381 gggtctgtgt tatggtgcta acacgttctt tcacgctgcc aaggcctaca tcgagggtta
     1441 ccacaccgtg ccgaagggcc ggtgtcgcca ggtggtgact ggcatggctt ggctcttctt
     1501 cgtatcatgg ggtatgttcc ccatcctgtt catcctcggc cccgagggct tcggcgtcct
     1561 gagcgtgtac ggctccaccg tcggccacac catcattgac ctgatgtcga agaactgctg
     1621 gggtctgctc ggccactacc tgcgcgtgct gatccacgag catatcctca tccacggcga
     1681 cattcgcaag accaccaaat tgaacattgg tggcactgag attgaggtcg agacgctggt
     1741 ggaggacgag gccgaggctg gcgcggtacc catggtgagc aagggcgagg agctgttcac
     1801 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     1861 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     1921 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     1981 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     2041 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     2101 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     2161 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     2221 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     2281 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     2341 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     2401 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     2461 cactctcggc atggacgagc tgtacaagtg ataagaattc gatatcaagc ttatcgataa
     2521 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     2581 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     2641 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     2701 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     2761 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     2821 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     2881 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     2941 ctatgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     3001 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     3061 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgagc
     3121 gctgctcgag agatctacgg gtggcatccc tgtgacccct ccccagtgcc tctcctggcc
     3181 ctggaagttg ccactccagt gcccaccagc cttgtcctaa taaaattaag ttgcatcatt
     3241 ttgtctgact aggtgtcctt ctataatatt atggggtgga ggggggtggt atggagcaag
     3301 gggcaagttg ggaagacaac ctgtagggcc tgcggggtct attgggaacc aagctggagt
     3361 gcagtggcac aatcttggct cactgcaatc tccgcctcct gggttcaagc gattctcctg
     3421 cctcagcctc ccgagttgtt gggattccag gcatgcatga ccaggctcag ctaatttttg
     3481 tttttttggt agagacgggg tttcaccata ttggccaggc tggtctccaa ctcctaatct
     3541 caggtgatct acccaccttg gcctcccaaa ttgctgggat tacaggcgtg aaccactgct
     3601 cccttccctg tccttctgat tttgtaggta accacgtgcg gaccgagcgg ccgcaggaac
     3661 ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc
     3721 gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc
     3781 gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     3841 cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc
     3901 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     3961 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     4021 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     4081 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     4141 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     4201 ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     4261 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
     4321 aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     4381 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     4441 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     4501 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     4561 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     4621 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     4681 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     4741 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     4801 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     4861 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     4921 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     4981 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     5041 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     5101 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     5161 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     5221 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     5281 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     5341 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     5401 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     5461 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     5521 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     5581 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     5641 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     5701 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     5761 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5821 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5881 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5941 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     6001 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     6061 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     6121 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6181 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     6241 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6301 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6361 cctggccttt tgctggcctt ttgctcacat gt
//
