LOCUS       Exported                6226 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    212_pAAV-ProD21-CatCh-GFP-WPRE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6226)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6226
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   12..141
                     /label=AAV2 ITR
     promoter        585..616
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      670..679
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             676..1599
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGA"
     CDS             1606..2322
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1651..1672)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1912..1931)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2259..2280
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2353..2941
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2406..2426)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2824..2835)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2973..3449
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3172..3191)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3489..3629
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3489..3618
                     /label=AAV2 ITR
     rep_origin      3704..4159
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3791..3810)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4001..4022
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4176..4195)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4295..4317
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4355..4373)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4441..4545
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4546..5406
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4764..4783)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5577..6165
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6066..6085
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtatcct ggggtttaaa caggactagg
      181 tccactggga gaagaggaag tcaaacagga ctagtcatcg ataggctagc cagcttaatt
      241 aatcagattt gtgagtgtgc aggcctggtg cagggcgggt tccggataat tgcacgtctc
      301 aggtgcgctg ggaggcaggg ggccagaggt cgatggggaa agcagccagg agggtagtta
      361 tctcctaatt attgcttgca ctggaatgtt ttattccatt tagaaaatgg tctgattgtt
      421 ttgacttttt tttttttttt catgctgtag gaaaacagcc aggtggggta gatccaagct
      481 gtgtgggtgg tgttctgtaa ttgctgggct ggactttctt ttgccaatta gatggcttcc
      541 tgagacctgc ccttttcatc ctcacatggt cctgctggag ttagtagagg gtatataatg
      601 gaagctcgac ttccagctat cacatccact gtgttgttgt gaactggaat ccactatagg
      661 ccaggatccg ccaccatgga ttatggaggc gccctgagtg ccgttgggcg cgagctgcta
      721 tttgtaacga acccagtagt cgtcaatggc tctgtacttg tgcctgagga ccagtgttac
      781 tgcgcgggct ggattgagtc gcgtggcaca aacggtgccc aaacggcgtc gaacgtgctg
      841 caatggcttg ctgctggctt ctccatccta ctgcttatgt tttacgccta ccaaacatgg
      901 aagtcaacct gcggctggga ggagatctat gtgtgcgcta tcgagatggt caaggtgatt
      961 ctcgagttct tcttcgagtt taagaacccg tccatgctgt atctagccac aggccaccgc
     1021 gtccagtggt tgcgttacgc cgagtggctt ctcacctgcc cggtcatttg cattcacctg
     1081 tcaaacctga cgggcttgtc caacgactac agcaggcgca ccatgggtct gcttgtgtct
     1141 gatattggca caattgtgtg gggcgccact tccgccatgg ccaccggata cgtcaaggtc
     1201 atcttcttct gcctgggtct gtgttatggt gctaacacgt tctttcacgc tgccaaggcc
     1261 tacatcgagg gttaccacac cgtgccgaag ggccggtgtc gccaggtggt gactggcatg
     1321 gcttggctct tcttcgtatc atggggtatg ttccccatcc tgttcatcct cggccccgag
     1381 ggcttcggcg tcctgagcgt gtacggctcc accgtcggcc acaccatcat tgacctgatg
     1441 tcgaagaact gctggggtct gctcggccac tacctgcgcg tgctgatcca cgagcatatc
     1501 ctcatccacg gcgacattcg caagaccacc aaattgaaca ttggtggcac tgagattgag
     1561 gtcgagacgc tggtggagga cgaggccgag gctggcgcgg tacccatggt gagcaagggc
     1621 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     1681 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     1741 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     1801 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     1861 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     1921 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     1981 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     2041 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     2101 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     2161 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     2221 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     2281 accgccgccg ggatcactct cggcatggac gagctgtaca agtgataaga attcgatatc
     2341 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     2401 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     2461 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     2521 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     2581 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     2641 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     2701 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     2761 ccttggctgc tcgcctatgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     2821 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     2881 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     2941 catcgatacc gagcgctgct cgagagatct acgggtggca tccctgtgac ccctccccag
     3001 tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc ctaataaaat
     3061 taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg tggagggggg
     3121 tggtatggag caaggggcaa gttgggaaga caacctgtag ggcctgcggg gtctattggg
     3181 aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc tcctgggttc
     3241 aagcgattct cctgcctcag cctcccgagt tgttgggatt ccaggcatgc atgaccaggc
     3301 tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc aggctggtct
     3361 ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg ggattacagg
     3421 cgtgaaccac tgctcccttc cctgtccttc tgattttgta ggtaaccacg tgcggaccga
     3481 gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct
     3541 cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt
     3601 gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca
     3661 tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc
     3721 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     3781 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     3841 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     3901 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     3961 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     4021 ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt
     4081 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     4141 atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag
     4201 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     4261 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     4321 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     4381 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     4441 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     4501 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     4561 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     4621 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     4681 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     4741 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     4801 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     4861 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     4921 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     4981 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     5041 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     5101 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     5161 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     5221 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     5281 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     5341 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     5401 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     5461 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     5521 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     5581 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     5641 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     5701 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     5761 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     5821 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     5881 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     5941 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     6001 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6061 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6121 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6181 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgt
//
