LOCUS       Exported                6733 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    216_pAAV-ProD24-CatCh-GFP-WPRE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6733)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6733
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   12..141
                     /label=AAV2 ITR
     promoter        467..498
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     promoter        1092..1123
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      1177..1186
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1183..2106
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGA"
     CDS             2113..2829
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2158..2179)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2419..2438)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2766..2787
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2860..3448
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2913..2933)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3331..3342)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3480..3956
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3679..3698)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3996..4136
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3996..4125
                     /label=AAV2 ITR
     rep_origin      4211..4666
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4298..4317)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4508..4529
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4683..4702)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4802..4824
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4862..4880)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4948..5052
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5053..5913
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5271..5290)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6084..6672
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6573..6592
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtatcct ggggtttaaa caggactagg
      181 tccactggga gaagaggaag tcaaacagga ctagtcatcg ataggctagc cagcttaatt
      241 aatcagatat tccggtcaca cggccaagat tattccacct gcgctttgag caatagggag
      301 agggctctgg tgcctcttcc tggaatttga ttaattcgct tgagtcagtc acagaatttg
      361 aggaagcatt gatatttgaa gatgtgttct tctaaaggat acaaatgaat atatgcatag
      421 tgagagttta ggagatagca tcctcacatg gtcctgctgg agttagtaga gggtatataa
      481 tggaagctcg acttccagct atcacatcca ctgtgttgtt gtgaactgga atccactata
      541 ggccaatcct ggggtttaaa caggactagg tccactggga gaagaggaag tcaaacagga
      601 ctagtcatcg ataggctagc cagcttaatt aatcagatgg ttcattgtaa gccactgtag
      661 tcgtgggtga ctcacatcaa accacccctt cgggaacacg atgccgacac tgaaactaca
      721 taggggagag caaataaaac tgtcttctct agtttgggga aattggagcc tgccttaggg
      781 aaacagtgac taatttacag ctagtgtttg gaatgagatc atctgtcaaa ataaatgtat
      841 ctttacttcc ttcttgagag gaagcaagct gttttatgat gttccttgga atccttaaaa
      901 atacagagca acatttacat tattaatgat acgctttatt gctgcaggct aactaggtcc
      961 aaaattgtcc ttccatagat ggagctggag agttacacag aagtttgcat atcgagctct
     1021 taggtctgca tgtacagcta atgtacttgt ggaccctgtc acatatcctc acatggtcct
     1081 gctggagtta gtagagggta tataatggaa gctcgacttc cagctatcac atccactgtg
     1141 ttgttgtgaa ctggaatcca ctataggcca ggatccgcca ccatggatta tggaggcgcc
     1201 ctgagtgccg ttgggcgcga gctgctattt gtaacgaacc cagtagtcgt caatggctct
     1261 gtacttgtgc ctgaggacca gtgttactgc gcgggctgga ttgagtcgcg tggcacaaac
     1321 ggtgcccaaa cggcgtcgaa cgtgctgcaa tggcttgctg ctggcttctc catcctactg
     1381 cttatgtttt acgcctacca aacatggaag tcaacctgcg gctgggagga gatctatgtg
     1441 tgcgctatcg agatggtcaa ggtgattctc gagttcttct tcgagtttaa gaacccgtcc
     1501 atgctgtatc tagccacagg ccaccgcgtc cagtggttgc gttacgccga gtggcttctc
     1561 acctgcccgg tcatttgcat tcacctgtca aacctgacgg gcttgtccaa cgactacagc
     1621 aggcgcacca tgggtctgct tgtgtctgat attggcacaa ttgtgtgggg cgccacttcc
     1681 gccatggcca ccggatacgt caaggtcatc ttcttctgcc tgggtctgtg ttatggtgct
     1741 aacacgttct ttcacgctgc caaggcctac atcgagggtt accacaccgt gccgaagggc
     1801 cggtgtcgcc aggtggtgac tggcatggct tggctcttct tcgtatcatg gggtatgttc
     1861 cccatcctgt tcatcctcgg ccccgagggc ttcggcgtcc tgagcgtgta cggctccacc
     1921 gtcggccaca ccatcattga cctgatgtcg aagaactgct ggggtctgct cggccactac
     1981 ctgcgcgtgc tgatccacga gcatatcctc atccacggcg acattcgcaa gaccaccaaa
     2041 ttgaacattg gtggcactga gattgaggtc gagacgctgg tggaggacga ggccgaggct
     2101 ggcgcggtac ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     2161 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     2221 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     2281 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     2341 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     2401 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     2461 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     2521 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     2581 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     2641 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     2701 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     2761 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     2821 ctgtacaagt gataagaatt cgatatcaag cttatcgata atcaacctct ggattacaaa
     2881 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     2941 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     3001 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     3061 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     3121 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     3181 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     3241 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctatgttgc cacctggatt
     3301 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     3361 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     3421 cggatctccc tttgggccgc ctccccgcat cgataccgag cgctgctcga gagatctacg
     3481 ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag
     3541 tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct
     3601 tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa
     3661 cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc
     3721 tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt
     3781 tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg
     3841 gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt
     3901 ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctga
     3961 ttttgtaggt aaccacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc
     4021 cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg
     4081 cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg
     4141 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag
     4201 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     4261 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     4321 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     4381 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
     4441 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     4501 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc gggctattct
     4561 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     4621 caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc
     4681 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     4741 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     4801 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     4861 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     4921 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     4981 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     5041 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     5101 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     5161 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     5221 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     5281 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     5341 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     5401 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     5461 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     5521 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     5581 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     5641 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     5701 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     5761 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     5821 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     5881 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     5941 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     6001 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     6061 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6121 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     6181 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     6241 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     6301 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     6361 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     6421 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     6481 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     6541 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     6601 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     6661 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     6721 tttgctcaca tgt
//
