LOCUS Exported 7908 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina. ACCESSION . VERSION . KEYWORDS 217_pAAV-ProD25-CatCh-GFP-WPRE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7908) AUTHORS Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B TITLE Targeting neuronal and glial cell types with synthetic promoter AAVs in mice, non-human primates and humans. JOURNAL Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 10.1038/s41593-019-0431-2. PUBMED 31285614 REFERENCE 2 (bases 1 to 7908) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7908 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..141 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 12..141 /label=AAV2 ITR regulatory 2352..2361 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 2358..3281 /codon_start=1 /product="N-terminal fragment of channelrhodopsin-2 from the alga Chlamydomonas reinhardtii (Nagel et al., 2003)" /label=ChR2 /note="blue light-activated cation channel" /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI RKTTKLNIGGTEIEVETLVEDEAEAGA" CDS 3288..4004 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(3333..3354) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(3594..3613) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 3941..3962 /label=EGFP-C /note="EGFP, forward primer" misc_feature 4035..4623 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(4088..4108) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(4506..4517) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 4655..5131 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(4854..4873) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" repeat_region 5171..5311 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 5171..5300 /label=AAV2 ITR rep_origin 5386..5841 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5473..5492) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5683..5704 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(5858..5877) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 5977..5999 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(6037..6055) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 6123..6227 /gene="bla" /label=AmpR promoter CDS 6228..7088 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(6446..6465) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 7259..7847 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7748..7767 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtattcc ggtcacacgg ccaagattat 181 tccacctgcg ctttgagcaa tagggagagg gctctggtgc ctcttcctgg aatttgatta 241 attcgcttga gtcagtcaca gaatttgagg aagcattgat atttgaagat gtgttcttct 301 aaaggataca aatgaatata tgcatagtga gagtttagga gatagagagg caggccgagt 361 ttgaggccag cctggtctac acaggcgttc taggagaacc tgtctcatat gcacatgggc 421 ctgggattac acatacaact gcaatggcag cacttggaaa gcggaagcag gagaatcgga 481 atttcacact catccttcat tatagaagtc caaacctgtg ctaagctact tggacatcgc 541 taaaaaacag caaaaatctt tctaggaatt gccaatgtat acacaccaag ttgtattttt 601 gtagggcaga cttttccaac ttgccagatg ataataatca tttatattag ctacatttca 661 ggctccagaa ttaacactgt tactgaaatg tataggttct aaaacatacc atttccaaat 721 attttaaagg attaacattt ttgaaaagca tgtgatcttc caaccatatt ttagggaaca 781 gacataagaa gtaggcaagt ttgggaagaa acagttcctg agggcatttc ttcccaacct 841 tgaatgccct gtgggttaac tgaggtcttg gtaaaatgta gattatttac tggacttttt 901 gaactgagag tgcatttcta acaaaactcc agggtatgtg gtccttgcat tacatatggg 961 gtagcaacat tctaaagcag tgtttttcaa cctgtggatc aagacccaca gaagtcacat 1021 agcagatatc ctgcctatta gatatttaca ttaagattca gagcagtagc agaataggag 1081 tcatcccaac ctgaggaact gcatcagagt cccagcatca ggaagggtga gagccactga 1141 gctaaagtcc tctaggtgag ggggctgccc cggaaagact catttaaatg aaaccactga 1201 cacagagagc tgacagatga ggtgggttcc gtgtctgtga ggctctgctg gtcgtctcct 1261 cactcccatg gaagaaccac cgagatgagg gcgaggggca gagctaaccc agcctctaag 1321 taggcagagt ctagtgtcca gctgcccaag gaagaagttt gctgtgtgag gtggccctga 1381 tgtccgcaca cacaaaatgc cagtgaagtc tacttgacca agtgaagctg gtgtggaatg 1441 ggaagaagca cacacagtca gtctctctgc acacactctg tccttcactt cttcacttac 1501 cagagatttg atgagaacct actagcagat cagatttgat ccctgagtgg aaaaaacgta 1561 cagtgggaga taaaagagga aaacaacgga tctgagtttg aggttagcct agtctgagca 1621 agccggatac acagtaagac cctgtctcac atacatccac tcgcacgcgc gcgcacacaa 1681 acacacacac acacacacac acacacacac acacacacac acatcgtgct aaggacaaga 1741 taggcatcct gagagatgag ccaggacaaa gaaccagtaa tagctcctgg agcagcacat 1801 ctgttttgcc aggattatcc cttggatctc ttaaaaccga gaccttgtaa tctgaagact 1861 caacttgggc tgtaccctta accttcagct ctatgatgca agtgagtcca caggaccgga 1921 ggctttgaga tgagcttttc agaagggagg agttggccgc ttgctcccag agctccagca 1981 cctgcattct tctggctatg tcagaagcca gatcatttcc ctcgttaaaa acaaaaacaa 2041 aaaaacaaac aaacaaaatg ttagtctttg ccctttatct gcctggcaaa gcttttaatt 2101 ggcttgatct gtcattccgc tagacataaa ggggacaatc cccggattag gaaggagctc 2161 tccagctcgg gtaaggagtc tcaaggcaag gtaggcaagc accaccggtc cgcactctcg 2221 cccagctttt acgggaagaa gagaatgtta ctctatccta acatattttt ccttttcctc 2281 tatctcacag ataggaaaaa tttaagagcc agagggaacg tcccttctca gaggagacag 2341 cagaaggatc cgccaccatg gattatggag gcgccctgag tgccgttggg cgcgagctgc 2401 tatttgtaac gaacccagta gtcgtcaatg gctctgtact tgtgcctgag gaccagtgtt 2461 actgcgcggg ctggattgag tcgcgtggca caaacggtgc ccaaacggcg tcgaacgtgc 2521 tgcaatggct tgctgctggc ttctccatcc tactgcttat gttttacgcc taccaaacat 2581 ggaagtcaac ctgcggctgg gaggagatct atgtgtgcgc tatcgagatg gtcaaggtga 2641 ttctcgagtt cttcttcgag tttaagaacc cgtccatgct gtatctagcc acaggccacc 2701 gcgtccagtg gttgcgttac gccgagtggc ttctcacctg cccggtcatt tgcattcacc 2761 tgtcaaacct gacgggcttg tccaacgact acagcaggcg caccatgggt ctgcttgtgt 2821 ctgatattgg cacaattgtg tggggcgcca cttccgccat ggccaccgga tacgtcaagg 2881 tcatcttctt ctgcctgggt ctgtgttatg gtgctaacac gttctttcac gctgccaagg 2941 cctacatcga gggttaccac accgtgccga agggccggtg tcgccaggtg gtgactggca 3001 tggcttggct cttcttcgta tcatggggta tgttccccat cctgttcatc ctcggccccg 3061 agggcttcgg cgtcctgagc gtgtacggct ccaccgtcgg ccacaccatc attgacctga 3121 tgtcgaagaa ctgctggggt ctgctcggcc actacctgcg cgtgctgatc cacgagcata 3181 tcctcatcca cggcgacatt cgcaagacca ccaaattgaa cattggtggc actgagattg 3241 aggtcgagac gctggtggag gacgaggccg aggctggcgc ggtacccatg gtgagcaagg 3301 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg 3361 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc 3421 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc 3481 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct 3541 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg 3601 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg 3661 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca 3721 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga 3781 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc 3841 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc 3901 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg 3961 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtgataa gaattcgata 4021 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc 4081 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg 4141 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc 4201 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg 4261 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg 4321 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga 4381 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct 4441 ttccttggct gctcgcctat gttgccacct ggattctgcg cgggacgtcc ttctgctacg 4501 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc 4561 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc 4621 cgcatcgata ccgagcgctg ctcgagagat ctacgggtgg catccctgtg acccctcccc 4681 agtgcctctc ctggccctgg aagttgccac tccagtgccc accagccttg tcctaataaa 4741 attaagttgc atcattttgt ctgactaggt gtccttctat aatattatgg ggtggagggg 4801 ggtggtatgg agcaaggggc aagttgggaa gacaacctgt agggcctgcg gggtctattg 4861 ggaaccaagc tggagtgcag tggcacaatc ttggctcact gcaatctccg cctcctgggt 4921 tcaagcgatt ctcctgcctc agcctcccga gttgttggga ttccaggcat gcatgaccag 4981 gctcagctaa tttttgtttt tttggtagag acggggtttc accatattgg ccaggctggt 5041 ctccaactcc taatctcagg tgatctaccc accttggcct cccaaattgc tgggattaca 5101 ggcgtgaacc actgctccct tccctgtcct tctgattttg taggtaacca cgtgcggacc 5161 gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg 5221 ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca 5281 gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg 5341 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg 5401 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 5461 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 5521 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc 5581 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga 5641 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 5701 ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg attttgccga 5761 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca 5821 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat 5881 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc 5941 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt 6001 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat 6061 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg 6121 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 6181 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 6241 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 6301 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 6361 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 6421 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg 6481 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 6541 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 6601 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 6661 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 6721 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg 6781 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 6841 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 6901 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 6961 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 7021 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 7081 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 7141 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 7201 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 7261 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 7321 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 7381 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca 7441 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 7501 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 7561 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 7621 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 7681 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 7741 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 7801 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 7861 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt //