LOCUS       Exported               11057 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Lentiviral expression of DDR2.
ACCESSION   .
VERSION     .
KEYWORDS    pHAGE-DDR2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11057)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11057
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             4344..4352
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    complement(6017..6041)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6049..6067
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6092..6111)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6093..6111)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(6117..6137)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             6191..6232
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6253..6820
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6270..6822
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6414..6431)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6641..6660
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6834..7433
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6834..6853)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7330..7350
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7447..8035
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7500..7520)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7918..7929)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8110..8343
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8462..8480)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8548..8652
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8653..9513
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8871..8890)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9684..10272
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10173..10192
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10426..10443
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     10535..10554
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     protein_bind    10683..10704
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10719..10749
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10757..10773
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10762..10784
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10781..10797
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10781..10797
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10847..10866
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg atcctgattc ccagaatgct cttggtgctg
     3481 ttcctgctgc tgcctatctt gagttctgca aaagctcagg ttaatccagc tatatgccgc
     3541 tatcctctgg gcatgtcagg aggccagatt ccagatgagg acatcacagc ttccagtcag
     3601 tggtcagagt ccacagctgc caaatatgga aggctggact cagaagaagg ggatggagcc
     3661 tggtgccctg agattccagt ggaacctgat gacctgaagg agtttctgca gattgacttg
     3721 cacaccctcc attttatcac tctggtgggg acccaggggc gccatgcagg aggtcatggc
     3781 atcgagtttg cccccatgta caagatcaat tacagtcggg atggcactcg ctggatctct
     3841 tggcggaacc gtcatgggaa acaggtgctg gatggaaata gtaaccccta tgacattttc
     3901 ctaaaggact tggagccgcc cattgtagcc agatttgtcc ggttcattcc agtcaccgac
     3961 cactccatga atgtgtgtat gagagtggag ctttacggct gtgtctggct agatggcttg
     4021 gtgtcttaca atgctccagc tgggcagcag tttgtactcc ctggaggttc catcatttat
     4081 ctgaatgatt ctgtctatga tggagctgtt ggatacagca tgacagaagg gctaggccaa
     4141 ttgaccgatg gtgtgtctgg cctggacgat ttcacccaga cccacgaata ccacgtgtgg
     4201 cccggctatg actatgtggg ctggcggaac gagagtgcca ccaatggcta cattgagatc
     4261 atgtttgaat ttgaccgcat caggaatttc actaccatga aggtccactg caacaacatg
     4321 tttgctaaag gtgtgaagat ctttaaggag gtacagtgct acttccgctc tgaagccagt
     4381 gagtgggaac ctaatgccat ttccttcccc cttgtcctgg atgacgtcaa ccccagtgct
     4441 cggtttgtca cggtgcctct ccaccaccga atggccagtg ccatcaagtg tcaataccat
     4501 tttgcagata cctggatgat gttcagtgag atcaccttcc aatcagatgc tgcaatgtac
     4561 aacaactctg aagccctgcc cacctctcct atggcaccca caacctatga tccaatgctt
     4621 aaagttgatg acagcaacac tcggatcctg attggctgct tggtggccat catctttatc
     4681 ctcctggcca tcattgtcat catcctctgg aggcagttct ggcagaaaat gctggagaag
     4741 gcttctcgga ggatgctgga tgatgaaatg acagtcagcc tttccctgcc aagtgattct
     4801 agcatgttca acaataaccg ctcctcatca cctagtgaac aagggtccaa ctcgacttac
     4861 gatcgcatct ttccccttcg ccctgactac caggagccat ccaggctgat acgaaaactc
     4921 ccagaatttg ctccagggga ggaggagtca ggctgcagcg gtgttgtgaa gccagtccag
     4981 cccagtggcc ctgagggggt gccccactat gcagaggctg acatagtgaa cctccaagga
     5041 gtgacaggag gcaacacata ctcagtgcct gccgtcacca tggacctgct ctcaggaaaa
     5101 gatgtggctg tggaggagtt ccccaggaaa ctcctaactt tcaaagagaa gctgggagaa
     5161 ggacagtttg gggaggttca tctctgtgaa gtggagggaa tggaaaaatt caaagacaaa
     5221 gattttgccc tagatgtcag tgccaaccag cctgtcctgg tggctgtgaa aatgctccga
     5281 gcagatgcca acaagaatgc caggaatgat tttcttaagg agataaagat catgtctcgg
     5341 ctcaaggacc caaacatcat ccatctatta gctgtgtgta tcactgatga ccctctctgt
     5401 atgatcactg aatacatgga gaatggagat ctcaatcagt ttctttcccg ccacgagccc
     5461 cctaattctt cctccagcga tgtacgcact gtcagttaca ccaatctgaa gtttatggct
     5521 acccaaattg cctctggcat gaagtacctt tcctctctta attttgttca ccgagatctg
     5581 gccacacgaa actgtttagt gggtaagaac tacacaatca agatagctga ctttggaatg
     5641 agcaggaacc tgtacagtgg tgactattac cggatccagg gccgggcagt gctccctatc
     5701 cgctggatgt cttgggagag tatcttgctg ggcaagttca ctacagcaag tgatgtgtgg
     5761 gcctttgggg ttactttgtg ggagactttc accttttgtc aagaacagcc ctattcccag
     5821 ctgtcagatg aacaggttat tgagaatact ggagagttct tccgagacca agggaggcag
     5881 acttacctcc ctcaaccagc catttgtcct gactctgtgt ataagctgat gctcagctgc
     5941 tggagaagag atacgaagaa ccgtccctca ttccaagaaa tccaccttct gctccttcaa
     6001 caaggcgacg agtagaaccc agctttcttg tacaaagtgg tgatatccaa ttaaccctca
     6061 ctaaagggaa agtttaagtg atgtatattg tccctatagt gagtcgtatt accacccaac
     6121 ttttctatac aaagtggttg atatccagca cagtggcggc cgctcgagtc tagagggccc
     6181 gcggttcgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg
     6241 ttagtaatga gatccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     6301 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     6361 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     6421 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     6481 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     6541 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     6601 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     6661 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     6721 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
     6781 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca catatgaccg
     6841 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     6901 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
     6961 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     7021 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     7081 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     7141 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     7201 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     7261 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     7321 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     7381 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc taaatcgata
     7441 gatcctaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     7501 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     7561 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     7621 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     7681 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     7741 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     7801 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     7861 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     7921 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     7981 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgag
     8041 atcctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     8101 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     8161 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     8221 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     8281 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     8341 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     8401 cagagagtga gaggcccggg ttaattaagg aaagggctag atcattcttg aagacgaaag
     8461 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     8521 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     8581 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     8641 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     8701 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     8761 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     8821 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     8881 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     8941 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     9001 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     9061 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     9121 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     9181 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     9241 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     9301 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     9361 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     9421 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     9481 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     9541 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     9601 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     9661 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     9721 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     9781 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     9841 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     9901 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     9961 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    10021 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    10081 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    10141 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    10201 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    10261 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    10321 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    10381 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    10441 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
    10501 taatgcagca agctcatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
    10561 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
    10621 aaaagctccc cgtggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
    10681 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
    10741 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg
    10801 attacgaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    10861 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
    10921 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
    10981 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
    11041 actacaaact tagtagt
//
