LOCUS Exported 12770 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION CRISPRi plasmid for use in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail). ACCESSION . VERSION . KEYWORDS pRS159 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12770) AUTHORS Wensing L, Sharma J, Uthayakumar D, Proteau Y, Chavez A, Shapiro RS TITLE A CRISPR Interference Platform for Efficient Genetic Repression in Candida albicans. JOURNAL mSphere. 2019 Feb 13;4(1). pii: 4/1/e00002-19. doi: 10.1128/mSphere.00002-19. PUBMED 30760609 REFERENCE 2 (bases 1 to 12770) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12770 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..17 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 6..28 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 25..41 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 25..41 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" promoter 62..80 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" CDS 1114..5217 /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=dCas9 /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the N863A mutation in the HNH catalytic domain" /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 5230..5250 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" terminator 6544..6741 /label=TEF terminator /note="Ashbya gossypii TEF terminator" promoter complement(7342..7685) /label=TEF promoter /note="Ashbya gossypii TEF promoter" primer_bind complement(8648..8667) /label=T7 /note="T7 promoter, forward primer" promoter complement(8649..8667) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(8674..8691) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(8674..8690) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8683..8705) /label=M13/pUC Forward /note="In lacZ gene" primer_bind complement(8919..8938) /label=F1ori-R /note="F1 origin, reverse primer" misc_RNA complement(8975..9049) /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" primer_bind 10501..10522 /label=F1ori-F /note="F1 origin, forward primer" promoter 10685..10789 /gene="bla" /label=AmpR promoter CDS 10790..11650 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(11008..11027) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 11821..12409 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 12310..12329 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 12563..12580 /label=L4440 /note="L4440 vector, forward primer" protein_bind 12697..12718 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 12733..12763 /label=lac promoter /note="promoter for the E. coli lac operon" ORIGIN 1 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg 61 aaattaaccc tcactaaagg gaacaaaagc tggtaccaga gctattaaga tcaccagcct 121 cgtttataat aaacttagtc taatatttct tttggaccaa aatagaaact gttctcaggt 181 ggcaaaaaac agcttaaagt ctgtgttaaa aaaagatctc ggctatgcca atcaaaaagg 241 agtgacatta ttatgtaaaa gaagaaatgt tgtaattccg cgaaacaaca attttaatgc 301 ttactgtgcc gccaccaata tcctatggcc aaggggcctt taaacatgcc acggaaatat 361 acagtcaaag taaacctact atgtctttag agccttcagg atcaattgtt tttaaatgaa 421 tgactgcaaa actagaaaag tagcctctta cataagcaag tcatttgtac accgagagtt 481 tagtaaccag tgaagcaagt atatctggca aacctgaaac ttatctgtga attataaagc 541 tcataagtaa aaagtgtaga aaagaagata aggcagacca aatacaaatt tgcaacagta 601 caacgaatat ttcagcgtca aaactagaga ataataaaga aaacgatctt ttcaaaaaga 661 aaaaaccttt tagttttcct ttgttgttgt tgtgggtgtg tgctatttat attatatagt 721 ttactcataa taccataaaa tattcggttt gattaggtta ttttaataag ctaatttgtt 781 tctaatcgtg taatttatgc tgtgtatatt aagtagtgtg tgcactgccc aaaaatgttt 841 gttgtttata gtcggttaaa gagaaaaaag aaaaaaagat ccatacacac acgttaatta 901 gttgttcaac gtaatacact catattttgt tcttatttgc tttcggtcgc tgttctcacc 961 aagatttatt gccaacgaaa caattttttt ttatatattt tcagattttt ctttttttcc 1021 tttcctttcc ttttctaatt ttcactcctg gttttctttc tttcttagaa acattatctc 1081 gatattaata ttaaaaaaat ataatcattc aaaatggata aaaaatactc cataggtttg 1141 gccattggaa ccaattcagt tggatgggct gtaatcactg atgaatataa ggttccttct 1201 aaaaagttca aagttttagg aaatactgat agacactcca ttaaaaagaa tttaattggt 1261 gccttgttgt ttgacagtgg tgaaacagct gaagcaactc gtttgaaaag aacagctaga 1321 agaagatata ccagaagaaa gaacagaatt tgttacttgc aagaaatctt ttctaatgaa 1381 atggcaaagg ttgatgattc tttttttcat agattggaag aaagtttctt agttgaagaa 1441 gataagaaac atgaaagaca tccaatcttt ggtaatattg tagatgaagt ggcttaccac 1501 gaaaaatatc ctacaattta tcatttgaga aaaaaattag ttgactccac tgataaagct 1561 gacttaagac ttatatattt ggcattggct cacatgatca agttcagagg tcacttctta 1621 attgaaggtg atttgaatcc agataattcc gatgtcgaca agttgtttat tcaattggtt 1681 caaacttaca accaactttt cgaggaaaat ccaatcaatg cctcaggtgt ggatgctaaa 1741 gctattttat ctgctagatt gtcaaaaagt agacgtttgg aaaatcttat tgcccaattg 1801 ccaggtgaaa aaaaaaatgg attgtttgga aacttaattg cattatcttt gggtttgacc 1861 ccaaatttca aatcaaactt tgatttggcc gaggatgcta aacttcaatt gtctaaggat 1921 acatacgatg acgatttaga taatcttctt gcccaaattg gtgatcaata tgcagattta 1981 ttcttggcag ctaagaactt gagtgatgca attttgttaa gtgacatttt gagagtcaac 2041 acagaaatta caaaagctcc tttatccgcc tctatgatta aaagatatga tgaacaccat 2101 caagatttaa ccttgttgaa agctcttgta cgtcaacaat taccagaaaa gtataaagaa 2161 atattctttg atcaatctaa aaatggttac gctggttata ttgatggtgg tgctagtcaa 2221 gaagaatttt ataagtttat caagccaatt cttgaaaaaa tggatggtac tgaggaattg 2281 ttggttaagt tgaatcgaga ggatttactt cgtaagcaaa gaacatttga taacggttct 2341 atccctcatc aaatccactt aggtgagtta cacgctatct tgagacgtca ggaagatttt 2401 tacccttttt tgaaagacaa tcgtgaaaaa attgaaaaaa ttttgacttt cagaattcct 2461 tattacgtcg ggccattggc tagaggtaat tctagatttg catggatgac tcgtaaatct 2521 gaagagacta tcaccccatg gaattttgag gaagttgttg ataagggagc ctccgctcaa 2581 tcattcatcg agagaatgac caatttcgac aaaaacttgc caaatgagaa ggtgttacct 2641 aaacattctt tgttatatga atatttcact gtttataatg aattaacaaa agtcaaatac 2701 gtcactgaag gtatgagaaa accagcattc ttaagtggtg aacaaaagaa agctatcgtt 2761 gacttgttat tcaaaactaa tcgtaaagtc actgtcaaac aacttaaaga ggactatttt 2821 aagaaaatag agtgtttcga ttccgttgaa atcagtgggg ttgaggatag atttaatgct 2881 agtttaggaa cttatcacga tttgttgaaa attatcaaag acaaagattt tttggataat 2941 gaagaaaacg aagatatctt ggaggacatt gtattgactt tgactttgtt tgaggataga 3001 gaaatgatcg aagaacgttt aaaaacttac gctcacttat tcgacgataa agtcatgaaa 3061 caattaaaaa gaagaagata taccggttgg ggaagattat ctagaaagtt gattaatggt 3121 attagagata agcaaagtgg taaaaccatc ttagatttcc ttaaaagtga tggttttgca 3181 aacagaaact ttatgcagtt gattcatgat gatagtttga cctttaagga agatattcaa 3241 aaagctcagg tttctggaca gggtgattca ttgcatgaac atattgccaa cttagcaggg 3301 tctccagcta tcaaaaaggg aattttacaa actgttaaag ttgttgatga gttggtgaaa 3361 gttatgggac gtcataagcc tgaaaatata gttatagaaa tggctagaga aaatcaaaca 3421 actcagaaag gacaaaagaa ttctcgtgaa agaatgaaaa gaatagaaga aggaattaaa 3481 gagttgggtt ctcaaatctt aaaagaacac ccagtcgaaa acacccagtt acagaacgag 3541 aagctttact tgtactattt gcaaaacggg cgtgacatgt atgtagatca agagttggat 3601 atcaatagac tttcagatta cgatgtcgat cacatagttc ctcagtcatt tttaaaagat 3661 gattccattg acaacaaagt tttaacaaga tctgataagg ctagaggtaa atcagacaat 3721 gtgccatcag aagaagtggt taagaaaatg aagaactatt ggagacaatt gttaaatgca 3781 aagttgatta ctcagagaaa atttgacaac ttaactaaag ctgaaagagg aggattatcc 3841 gaattagata aagctggatt cataaaacgt cagttggttg aaacaagaca aattactaaa 3901 catgtagccc aaattttaga ttcccgaatg aatactaaat atgatgaaaa tgacaaatta 3961 atcagagagg tcaaagtgat cacattaaag tcaaagttgg tgtcagattt ccgaaaggat 4021 tttcaattct acaaggtgag agagatcaac aattatcatc atgctcacga cgcatatttg 4081 aatgcagtgg taggaactgc cttaataaag aagtatccta agttagaatc cgaattcgtg 4141 tatggtgatt ataaagtcta cgatgtcaga aagatgatag caaaatcaga acaagaaatt 4201 gggaaagcca ccgcaaagta tttcttttac tccaacatca tgaatttctt taagaccgaa 4261 atcaccttgg ctaacgggga aatcagaaaa cgtcctttga ttgaaacaaa tggagaaacc 4321 ggtgaaattg tgtgggataa ggggagagat tttgctactg ttagaaaagt tttatcaatg 4381 cctcaagtta atattgtaaa gaagactgaa gttcaaactg gtggtttctc taaggaatcc 4441 attcttccaa aacgaaactc agacaagttg atcgcccgaa aaaaagattg ggatcctaag 4501 aaatacggag gttttgattc tcctactgtc gcatattctg tcttggtcgt ggctaaagtt 4561 gaaaagggta aatccaaaaa attgaaatct gtgaaagaat tgttaggtat taccatcatg 4621 gagcgttcat cctttgagaa aaacccaatt gactttctcg aagcgaaggg ctataaagaa 4681 gtgaagaaag atttgattat taagttgcca aagtattctc tctttgaact tgaaaatggg 4741 agaaagagaa tgttggcttc agctggagaa ttgcaaaaag gaaacgagtt agctttgcct 4801 tcaaagtacg ttaatttctt atacttagca tctcattatg aaaagttgaa ggggtcccct 4861 gaagacaatg agcaaaagca attattcgta gaacaacata aacactattt ggatgagata 4921 attgaacaaa tctccgagtt ttctaaaaga gttattttgg ctgatgcaaa tttggataaa 4981 gttttatctg cttataataa gcatcgtgac aaaccaattc gagaacaagc tgaaaatatt 5041 atccacttgt tcacattgac taacttggga gctccagccg cgtttaagta ctttgacacc 5101 accattgacc gtaagagata tacttcaaca aaagaagtgt tagacgccac attgattcat 5161 cagtccataa ctggtttata tgaaactaga atcgatttgt cacaattggg gggtgatagc 5221 cgagcggatc ctaagaaaaa acgaaaagtc agtggtggtg gtagtgggtc cgggtcagga 5281 ggttcatcca agttaggagg ttcaggaggg agtatggaac gagttagaat gatcaacgta 5341 cagcgtttgt tagaggctgc agagtttttg gaaagacgag aacgagaatg cgagcatggt 5401 tacgcatctt ccttcccatc tatgccatcc ccacgaggga gtggaggggg gtctgggtcc 5461 ggttctggat ctgggagttc aaaattaggg ggatccgggg gatctatgga acgtgtccgt 5521 atgattaatg tccaaagatt attagaagcc gcagaatttt tagaaagacg agaaagagaa 5581 tgtgaacatg gttatgcatc atcatttcca agtatgcctt caccaagagg ttaacccggg 5641 ggatccgagt gaaattctgg aaatctggaa atctggtttt gtattcttgt tattcttctt 5701 tttgttatta catatataac ttgttacttt tttaaaaaaa tctttgttta ttttataaat 5761 atataaaact aaatttaaga agaagagaaa aatgttttat ttgagagatt gaaattttac 5821 ttgaatttag cttagctttt ataaagtatt attatgtaaa aaaacaaaac aaatatacat 5881 taaaaagtta agactataaa atagccaccc aaggcatttc tatatcttgt tgttgttgtt 5941 ttcatcttct gtatcagagg aacttatttt attattttcg tcacgggtat tttctcttgt 6001 ttgatgattc atcccattca ttccatcata aaattctaga aggaccacct ttgattgtaa 6061 atagtaataa ttaccaccct tatttaatta tttatttaac ttatttattt atttattata 6121 catatataca aatctaataa agtgaaaatc tcccccttca catttataat tggccagttt 6181 ttttcaaata agcattccaa ccagcatctc tatacctttt accttcaata tctggatctc 6241 ttcctttacc aaacaatcct ctaccaacaa tgataatatc agttccagtg ctaacaactt 6301 catcaacagt tctatattgt tgtcctaatc catcaccttt atcatctaat ccaactccag 6361 gtgtcataat aagccaatca aatccttctt cttgtccacc catatcacgt tgggcaataa 6421 atccaataac aaattcctta tcggatttag caatttcaac agttttttga gaatattctc 6481 catacgaatt cgagctcgtt taaacgtcga cactggatgg cggcgttagt atcgaatcga 6541 cagcagtata gcgaccagca ttcacatacg attgacgcat gatattactt tctgcgcact 6601 taacttcgca tctgggcaga tgatgtcgag gcgaaaaaaa atataaatca cgctaacatt 6661 tgattaaaat agaacaacta caatataaaa aaactataca aatgacaagt tcttgaaaac 6721 aagaatcttt ttattgtcag tactgactcg agttattatg gacatggcat agacatatac 6781 aaagcttgtt caccatcgga agcagtacca tcgtataaag cagtatccaa accacacaaa 6841 gtgaaaccca ttcttctata agcatgaata gctggagcat taacattggt aacttccaac 6901 cacaaatgac cagcacctct ttctctggcg aattcagtag ccaaacccat caaagctcta 6961 ccaacaccat gacctctatg ttctggagca acttcaatat cttcaacagt caatcttctg 7021 ttccaaccag aataagaaac aacaacgaaa ccagccaaat caccatcatc accataagca 7081 acgaaagttc tagaatctgg atcaccatct tcaccagcat cggattcatc atcggattca 7141 tcatctggga aaaccttagt caatggtgga tcaactggaa cttctctcaa agtgaaacca 7201 tcaccagtag cagtaactct aaaaacagta tcggtagtga aagaaccatc caaagcttca 7261 atagcttcag catcacctgg aacagaagtt ctgtatctat aagcagtatc atccaaagta 7321 gtagacataa ttgtaggatc cggttgttta tgttcggatg tgatgtgaga actgtatcct 7381 agcaagattt taaaaggaag tatatgaaag aagaacctca gtggcaaatc ctaacctttt 7441 atatttctct acaggggcgc ggcgtgggga caattcaacg cgtctgtgag gggagcgttt 7501 ccctgctcgc aggtctgcag cgaggagccg taatttttgc ttcgcgccgt gcggccatca 7561 aaatgtatgg atgcaaatga ttatacatgg ggatgtatgg gctaaatgta cgggcgacag 7621 tcacatcatg cccctgagct gcgcacgtca agactgtcaa ggagggtatt ctgggcctcc 7681 atgtcgctgg ccgggtgacc cggcggggac gaggcaagct tgatatgcta atgatcccac 7741 tgatgataat tcagctaaca tcaataaccc tcttggctct tggttggtgg tggtttcttt 7801 agctccctgt tttaatcctt caactactcc attcccagtg acaccatgag cattagtaat 7861 atctgcccaa ctactaattt tataaactcc accaatatat tgtttcttca cggtattacc 7921 aatatcagca aattttctat cttcaaaaat cataaattga tgtttacgtg aaagttctaa 7981 taatggttca atagtggatt cataggaaaa atcattgatt atatcaatat gagtcttgat 8041 taagcataca taaggaccca atttatcaat taattcaagg aattccttag tggtatcaac 8101 atcaattgat gcacataaat tggttttctt cagttccatt aatcgaaata atcgttgtgc 8161 tactggtgag gcatgagttt ctgctctctc actataggtc ttagtgttga ctgtcatatc 8221 ttttgtttgc tatttctatt tctatttctt tcttcaactg taaaaggggc gatatataaa 8281 ccagtgtgta tggggttgtt gctcaggaat tgatcaatgt ttaaaagaat ggttgaaatc 8341 atcattgaag aacaaaaaaa aagatttttt ctttcattat gttatctgta taatatgcag 8401 gaaataagat tgcaaaaaaa tagccgtttt tttggatact atcaaacaag aggaatgact 8461 aatacagaat tagtgttacg aatcaatggc actacagcaa ctttcaacaa aataatctac 8521 gttcaaaata taatatatac tattaaataa aatcaaataa taaaataatt ttaaagaaag 8581 tagtaccagc tctttttttt gtttccgttt ataccatcca aatcaattcc tattagtctc 8641 caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga 8701 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag 8761 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa 8821 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 8881 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 8941 ctttctcgcc acgttcgccg gcaaaaaaaa gcaccaccga ctcggtgcca ctttttcaag 9001 ttgataacgg actagcctta ttttaacttg ctatttctag ctctaaaact gaagagccat 9061 taatgctctt cctcgaaatt aaaaatagtt tacgcaagtc tcgaacttcc accacaattt 9121 aattgtaaga acgctgcgaa aacatattct ccaatcctat tgggccgaag tttcagctat 9181 gccaaaggca tcaaattttt ttttataaaa ttttccactt ttccatacca gtgaaattat 9241 cacgtgatgg agtgagtatc tataatattt gattgtttct ttgatgaggc aatactcgag 9301 tatagttatt gaccaatgac ttttaccggc taaacaagtg gtattcaagc acaattcagt 9361 tttctggatc tctaataatt gcaattgcaa ttgcttcaca tactctgttt ataattcagt 9421 ttctttctct tgatgttact gatatattaa gttaataaat accaataact ttttttcaaa 9481 aatcttctat tttcctttca attgattatt taaacacgaa tcgttaataa gctgtgattg 9541 ctttttctct ttctttttct tttctttgtt caagtttgaa aaaaaaaaaa aaaggtttct 9601 cttgttcgat gtagaattca gtgggttata gttgtaatga tatatggaat aaaaagaaag 9661 aaagagaaga gaaagagaag aagagaagaa ttaagtaaac gtgtattaga atttaaaaat 9721 taaaattcat gataatcttt aataataatt aactaagaat cacaaaattg aaaatttgaa 9781 agaaaaaaga tcactccaag gtcgtgtcaa gtgactagaa attcttaatt aaaatagtta 9841 taacaaaaaa agaaatttgt atataacaat taagtaaaag aaaaaaaagc aattcgcttt 9901 gtataataat acccagaaaa atatattcat atattcataa atcaaacatg aaatgaagaa 9961 tgctgaatca cttgatagga tttagttcca ttatggtact aagaagtaat attacttcta 10021 tgaaaaggtt gaatccgaga tcaaaattca tcttttcata tactctggag aaaaaaaaaa 10081 aagtaattgt agtaagaatg acaagtatca gttgagaaaa aaagtaacaa agagaaagct 10141 cggaggaggc tccccaaagg ttttatcacc aatggtggta ccactaaccc aagaacagaa 10201 aaagcattga tattttgaaa aatacaatac tcatctttta acccaaacct tttcttgtcc 10261 tttaaaactt ttatctcatg tctgtccaaa gtcatattgt cttactactt tctctccttc 10321 aacgtccttc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 10381 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 10441 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 10501 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 10561 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 10621 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa 10681 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat 10741 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca 10801 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca 10861 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta 10921 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt 10981 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc 11041 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc 11101 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc 11161 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa 11221 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga 11281 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat 11341 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca 11401 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc 11461 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat 11521 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag 11581 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa 11641 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca 11701 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc 11761 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 11821 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 11881 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 11941 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 12001 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 12061 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 12121 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 12181 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 12241 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 12301 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 12361 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 12421 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 12481 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg 12541 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcgtaag agcgcccaat 12601 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 12661 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta 12721 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa //